Nanotechniques in proteomics: Current status, promises and challenges

S, Ray; Chandra, Harini; Srivastava, Sanjeeva

doi:10.1016/j.bios.2010.04.010

Cited by 52 publications

(35 citation statements)

References 125 publications

(127 reference statements)

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“…This powder is the raw material for producing the electrode. CP (Carbon Paste) is produced by adding 100 (mg) of Carbon graphite powder to 36 (µl) of paraffin oil, according to [221][222][223][224][225][226][227][228][229][230][231]. The modified CP with Californium colloidal nanoparticles is produced by adding 300 (µl) of Californium colloidal nanoparticles to 100 (mg) of Carbon graphite powder and after evaporation of water in desiccator for 3 hours, 36 (µl) of paraffin oil is added to it.…”

Section: Other Characterization Methods Experimental Techniques and mentioning

confidence: 99%

Improving the Performance of Nano–Endofullerenes in Polyaniline Nanostructure–Based Biosensors by Covering Californium Colloidal Nanoparticles with Multi–Walled Carbon Nanotubes

Heidari¹

2018

JAN

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In the current paper, Glucose (Dextrose), Fructose and Galactose-oxidase enzymes are used as stabilization medium due to its more efficiency, ability for more accurate controlling the enzyme reaction, protecting against wasting of enzyme as well as simple and easy use and exchange of enzyme medium after performing some levels of surface modification and developing Nano Endohedral Fullerenes (Endofullerenes) on Californium plate. For better connecting and stabilizing the enzyme on the medium, the prepared medium is washed by high concentration Sulfuric Acid and Nitric Acid and a large volume of deionized water and for protecting enzyme from devastating effect of Californium and prohibiting them to become inactive, surface is covered with Cystamine before stabilization. Regarding the large size of Glucose (Dextrose), Fructose and Galactose-oxidase enzymes compared to surface of medium, a connective material with amid at one end and Pyrine at the other end is used as transfer agent and for stabilizing this connection, the prepared medium is placed into dimethylformamide (DMF) solution for a couple of hours. Activity of stabilized enzyme at 460 (nm) wavelength recorded by spectroscope was depicted against time to evaluate its stability in various times. The prepared medium, which has a large amount of Glucose (Dextrose), Fructose and Galactose-oxidase enzymes, can be used as electrode in sensors. Furthermore, qualitative and quantitative measurement of food components is of great importance due to high cost of traditional methods, in addition to tendency for more accurate and sensitive detecting of these components. Glucose (Dextrose), Fructose, Galactose and Cholesterol and Cholesterol are such compounds that they frequently measure. Various methods are used to detect these food elements. However, the necessity for accurate measurement of these two compounds with high sensitivity, especially for food health issue, leads to developing biological methods, especially biosensors. Among them, biosensors based on conductive polymer nanostructures, especially Polyaniline (PANI), have been recently interested due to their unique characteristics. The current paper aims to introduce and investigate the previously performed studies about Polyaniline (PANI)-based biosensors for detecting Glucose (Dextrose), Fructose, Galactose and Cholesterol and Cholesterol. In addition, Glucose (Dextrose), Fructose and Galactose-oxidase electrochemical sensor is one of the best methods for detecting low amount of Glucose (Dextrose), Fructose and Galactose and applying Californium colloidal nanoparticles as a supplementary material in the structure of biosensor can be effective for improving its efficiency and optimum performance.

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Section: Other Characterization Methods Experimental Techniques and mentioning

confidence: 99%

Improving the Performance of Nano–Endofullerenes in Polyaniline Nanostructure–Based Biosensors by Covering Californium Colloidal Nanoparticles with Multi–Walled Carbon Nanotubes

Heidari¹

2018

JAN

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“…17,18 These unique properties make QDs ideal probes compared with traditional fluorescent dyes and they have been widely used for DNA sequencing, protein molecular detection, and cell and tissue imaging. [19][20][21][22][23] QDs have also demonstrated great power in multiplexed analysis because they can be simultaneously excited without overlapping emission peaks. Previous studies demonstrated that these characteristics can offer convenience in the multiplex detection of biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay

Zhang

Fan

et al. 2017

IJN

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Background Accurate and reliable blood grouping is essential for safe blood transfusion. However, conventional methods are qualitative and use only single-antigen detection. We overcame these limitations by developing a simple, quantitative, and multiplexed detection method for blood grouping using quantum dots (QDs) and magnetic beads. Methods In the QD fluorescence assay (QFA), blood group A and B antigens were quantified using QD labeling and magnetic beads, and the blood groups were identified according to the R value (the value was calculated with the fluorescence intensity from dual QD labeling) of A and B antigens. The optimized performance of QFA was established by blood typing 791 clinical samples. Results Quantitative and multiplexed detection for blood group antigens can be completed within 35 min with more than 10 5 red blood cells. When conditions are optimized, the assay performance is satisfactory for weak samples. The coefficients of variation between and within days were less than 10% and the reproducibility was good. The ABO blood groups of 791 clinical samples were identified by QFA, and the accuracy obtained was 100% compared with the tube test. Receiver-operating characteristic curves revealed that the QFA has high sensitivity and specificity toward clinical samples, and the cutoff points of the R value of A and B antigens were 1.483 and 1.576, respectively. Conclusion In this study, we reported a novel quantitative and multiplexed method for the identification of ABO blood groups and presented an effective alternative for quantitative blood typing. This method can be used as an effective tool to improve blood typing and further guarantee clinical transfusion safety.

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“…In the last years, several procedures and applications have been developed in order to obtain simple devices for protein biomarker detection [1][2][3][4][5][6]. In this context, electrochemical immunosensors provide advanced platforms for biomarker analysis with the advantages of being easy to use, inexpensive, rapid and robust as well as offering multianalyte testing capability.…”

Section: Introductionmentioning

confidence: 99%

A New Electrochemical Multiplexed Assay for PSA Cancer Marker Detection

et al. 2010

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In this paper, two simple and sensitive approaches for prostate specific antigen (PSA) detection are presented, by using antibody modified paramagnetic microparticles coupled to multiplexed electrochemical platforms. The first proposed approach is based on 8-sensors screen-printed arrays as candidate for electrochemical transducers and a simple target capturing step by means of antibody-functionalised magnetic beads. In order to improve the performances of the immunosensing, a second approach is developed by using GRAVI-Cell. This is microfluidic-based affinity assay device for running bead-protocols. This innovative system combines a special chip containing eight polymer microchannels, with a portable, computer-controlled instrument. Both immunosensing strategies developed promise to be a sensitive, multiplexed tool for fast and easy PSA analysis.

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Nanotechniques in proteomics: Current status, promises and challenges

Cited by 52 publications

References 125 publications

Improving the Performance of Nano–Endofullerenes in Polyaniline Nanostructure–Based Biosensors by Covering Californium Colloidal Nanoparticles with Multi–Walled Carbon Nanotubes

Improving the Performance of Nano–Endofullerenes in Polyaniline Nanostructure–Based Biosensors by Covering Californium Colloidal Nanoparticles with Multi–Walled Carbon Nanotubes

Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay

A New Electrochemical Multiplexed Assay for PSA Cancer Marker Detection

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Nanotechniques in proteomics: Current status, promises and challenges

Cited by 52 publications

References 125 publications

Improving the Performance of Nano–Endofullerenes in Polyaniline Nanostructure–Based Biosensors by Covering Californium Colloidal Nanoparticles with Multi–Walled Carbon Nanotubes

Improving the Performance of Nano–Endofullerenes in Polyaniline Nanostructure–Based Biosensors by Covering Californium Colloidal Nanoparticles with Multi–Walled Carbon Nanotubes

Quantitative and multiplexed detection for blood typing based on quantum dot&ndash;magnetic bead assay

A New Electrochemical Multiplexed Assay for PSA Cancer Marker Detection

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Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay