Nanostructuring of Patterned Microelectrodes To Enhance the Sensitivity of Electrochemical Nucleic Acids Detection

Soleymani, Leyla; Fang, Zhichao; Sun, Xuping; Yang, Hong; Taft, Bradford J.; Sargent, Edward H.; Kelley, Shana O.

doi:10.1002/anie.200902439

Cited by 95 publications

(105 citation statements)

References 26 publications

(36 reference statements)

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“…We carried out Au electrodeposition at locations determined by the opened apertures to grow three-dimensional (3D) microstructures for subsequent biosensing. The microstructured sensors protruded from the surface and reached into the solution 16,21,35,36 , with their size and morphology programmed by the deposition time, applied potential, Au concentration, supporting electrolyte and overcoating protocol. Nanostructures increase the sensitivity of the assay significantly 22,37-40 , so we coated the Au structures with a thin layer of Pd to form finely nanostructured microelectrodes (NMEs) (Fig.…”

Section: Resultsmentioning

confidence: 99%

An electrochemical clamp assay for direct, rapid analysis of circulating nucleic acids in serum

et al. 2015

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The analysis of cell-free nucleic acids (cfNAs), which are present at significant levels in the blood of cancer patients, can reveal the mutational spectrum of a tumour without the need for invasive sampling of the tissue. However, this requires differentiation between the nucleic acids that originate from healthy cells and the mutated sequences shed by tumour cells. Here we report an electrochemical clamp assay that directly detects mutated sequences in patient serum. This is the first successful detection of cfNAs without the need for enzymatic amplification, a step that normally requires extensive sample processing and is prone to interference. The new chip-based assay reads out the presence of mutations within 15 minutes using a collection of oligonucleotides that sequester closely related sequences in solution, and thus allow only the mutated sequence to bind to a chip-based sensor. We demonstrate excellent levels of sensitivity and specificity and show that the clamp assay accurately detects mutated sequences in a collection of samples taken from lung cancer and melanoma patients.

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Section: Resultsmentioning

confidence: 99%

An electrochemical clamp assay for direct, rapid analysis of circulating nucleic acids in serum

et al. 2015

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“…Previous work, including our own, indicates that nanostructured morphologies can be tuned and subsequently exploited to enhance electrocatalytic performance. 42,44,45 Specifically, "needle-like" or "urchin-like" structures display favorable electrocatalytic activity because of their large surface area and desirable geometry (corners, edges, etc. 35 and cellulose paper 7 as well as three-dimensional graphene.…”

Section: 41mentioning

confidence: 99%

High Aspect Ratio Carbon Nanotube Membranes Decorated with Pt Nanoparticle Urchins for Micro Underwater Vehicle PropulsionviaH₂O₂Decomposition

et al. 2015

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The utility of unmanned micro underwater vehicles (MUVs) is paramount for exploring confined spaces, but their spatial agility is often impaired when maneuvers require burst-propulsion. Herein we develop highaspect ratio (150:1), multiwalled carbon nanotube microarray membranes (CNT-MMs) for propulsive, MUV thrust generation by the decomposition of hydrogen peroxide (H 2 O 2 ). The CNT-MMs are grown via chemical vapor deposition with diamond shaped pores (nominal diagonal dimensions of 4.5 × 9.0 μm) and subsequently decorated with urchin-like, platinum (Pt) nanoparticles via a facile, electroless, chemical deposition process. The Pt-CNT-MMs display robust, high catalytic ability with an effective activation energy of 26.96 kJ mol -1 capable of producing a thrust of 0.209 ± 0.049 N from 50% [w/w] H 2 O 2 decomposition within a compact reaction chamber of eight Pt-CNT-MMs in series. A n upward trend in the research and use of unmanned underwater vehicles (UUVs), and in particular micro underwater vehicles (MUVs, small UUVS between 1 and 50 cm in length), for exploration of confined spaces such as ship wrecks, submerged oil pipelines, and various military purposes has been observed over recent years. 1À3 The locomotion of these vehicles is typically controlled by propellerbased systems, which are often used for long-endurance missions. 4À6 However, propeller-based systems are usually limited in their ability to perform tight radius turns, burst-driven docking maneuvers, and lowspeed course corrections. ABSTRACT The utility of unmanned micro underwater vehicles (MUVs) is paramount for exploring confined spaces, but their spatial

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“…Motivated by previous reports showing that nanostructuring can provide major improvements in the detection limits of biosensors, [23][24][25] we sought to incorporate such nanostructures on our microelectrodes. We found that elec- trodeposition of Au, Pd, and Pt resulted in nanostructured microelectrodes when a suitable plating potential and supporting electrolyte was chosen.…”

Section: Integrated Nanostructures For Direct Detection Of Dna At Attmentioning

confidence: 99%

Integrated nanostructures for direct detection of DNA at attomolar concentrations

Soleymani

Fang

Sargent

2009

Applied Physics Letters

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We report an integrated chip that senses nucleic acid biomarkers at exceptionally low concentrations. To achieve such sensitivities we exploit four concepts. (1) Nanostructured electrodes allow efficient display of probe sequences. (2) The use of uncharged probe sequences lowers the background signal in our read-out system. (3) Electrocatalysis provides built-in amplification of the electrical signal that reports hybridization events. (4) An optimal self-assembled monolayer of thiol-functionalized probe molecules is best achieved with the aid of a short spacer molecule to confer enhanced accessibility. We show herein that via joint optimization along these four axes we achieve attomolar sensitivity.

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Nanostructuring of Patterned Microelectrodes To Enhance the Sensitivity of Electrochemical Nucleic Acids Detection

Cited by 95 publications

References 26 publications

An electrochemical clamp assay for direct, rapid analysis of circulating nucleic acids in serum

An electrochemical clamp assay for direct, rapid analysis of circulating nucleic acids in serum

High Aspect Ratio Carbon Nanotube Membranes Decorated with Pt Nanoparticle Urchins for Micro Underwater Vehicle PropulsionviaH₂O₂Decomposition

Integrated nanostructures for direct detection of DNA at attomolar concentrations

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Nanostructuring of Patterned Microelectrodes To Enhance the Sensitivity of Electrochemical Nucleic Acids Detection

Cited by 95 publications

References 26 publications

An electrochemical clamp assay for direct, rapid analysis of circulating nucleic acids in serum

An electrochemical clamp assay for direct, rapid analysis of circulating nucleic acids in serum

High Aspect Ratio Carbon Nanotube Membranes Decorated with Pt Nanoparticle Urchins for Micro Underwater Vehicle PropulsionviaH2O2Decomposition

Integrated nanostructures for direct detection of DNA at attomolar concentrations

Contact Info

Product

Resources

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High Aspect Ratio Carbon Nanotube Membranes Decorated with Pt Nanoparticle Urchins for Micro Underwater Vehicle PropulsionviaH₂O₂Decomposition