Nanostructures and Nanoconstructions based on DNA

Yevdokimov, Yu. M.; Salyanov, V. I.; Skuridin, S. G.

doi:10.1201/b12154

Cited by 13 publications

(21 citation statements)

References 2 publications

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“…The stability of "rigid" DNA particles depends on the properties of the nanobridges, not on the osmotic pressure of solution [15]. The result of the AFM studies of the "rigid" DNA particles allows one to perform some important evaluations [3]. It was confirmed that one "rigid" particle contains 1.6 l0 4 ds DNA molecules, the molecular mass of one DNA "rigid" particle is equal to (1.0-1.2) 10 10 Da.…”

Section: Resultsmentioning

confidence: 99%

“…In this case, the twist angle between layers (~ 0.1 [4]) may vanish, which is equivalent to unwinding of the cholesteric structure of the dispersion [7]. Moreover, one can suppose that nano-Au with the size comparable to the distance [3,4] between DNA molecules in quasinematic layers can diffuse between these molecules (Fig. 2).…”

Section: Changes In CD Spectra Caused By the Doping Of Dna Clcd Partimentioning

confidence: 99%

“…Besides, these molecules maintain almost all of the essential diffusion freedom, they exist in a "soluble" state, i.e., they retain the liquid properties. Such combination of properties allows this structure to be defined as "liquid-crystalline" (see [3,4] and early references cited therein). (Every neighboring layer in this structure is called a "quasinematic layer" in the physics of liquid crystals [5]).…”

Section: Introductionmentioning

confidence: 99%

“…However, in physicochemical terms the transformation of "liquid" particles of the DNA liquid-crystalline dispersions (LCD) into a "rigid" (gel-like) state seems to be more perspective in comparison to the insolubilization of individual DNA molecules [1,3].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Physicochemical and Nanotechnological Approaches to Creation of “Rigid” DNA Nanoconstructions

Yevdokimov¹,

Salyanov²,

Штыкова³

et al. 2014

TONANOJ

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Abstract:Two different approaches to the creation of "rigid" (gel-like) DNA particles (or DNA nanoconstructions) are considered. The physicochemical approach is based on the formation of nanobridges between double-stranded DNA molecules ordered in quasinematic layers of cholesteric liquid-crystalline dispersion (CLCD) particles or their salting-out. This approach represents by itself a "chemical gelation" of DNA molecules realized inside the nanometric DNA particles. The formed "rigid" DNA particles have unique physicochemical properties. The nanotechnological approach is based on induction of "physical gelation" as a result of the formation of Au-clusters in the "free" space between double-stranded DNA molecules ordered in CLCD particles. This approach results in the formation of a new "rigid" DNA material. Its distinctive peculiarity, in contrast to the chemically bonded "rigid" DNA particles, consists in a very weak binding affinity to the nuclear membrane filter used for atomic force microscopy. This opens up a possibility for easy manipulations with CLCD particles doped with gold nanoparticles.Keywords: DNA liquid-crystalline dispersions, nanoconstructions of DNA, nanotechnology of nucleic acids, structure of DNA liquid crystals.

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Section: Resultsmentioning

confidence: 99%

Section: Changes In CD Spectra Caused By the Doping Of Dna Clcd Partimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Physicochemical and Nanotechnological Approaches to Creation of “Rigid” DNA Nanoconstructions

Yevdokimov¹,

Salyanov²,

Штыкова³

et al. 2014

TONANOJ

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show abstract

“…The mobile and inexpensive device is intended for use in a biosensor analytical system for rapid determination of biologically active compounds in liquids to solve practical problems of clinic medicine and pharmacology.We develop optical biosensor analytical systems, in which "liquid" particles of the cholesteric liquid-crystalline dispersion (CLCD) of DNA are used as a biosensing unit, and a portable dichrometer registers changes of the circular dichroism (CD) signal generated with the DNA biosensing unit during its interaction with a defined biologically active compound (BAC) [1]. DNA biosensing units exhibit anomalously large optical activity in the CD spectrum in an absorption band of DNA nitrogen bases (chromophores) at 270 nm, the magnitude of which is stable and acts as a convenient "quality" criterion of prepared DNA CLCD particles.…”

mentioning

confidence: 99%

A compact two-wave dichrometer of an optical biosensor analytical system for medicine

et al. 2016

Self Cite

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An experimental model has been developed of a compact twowave dichrometer on the base of LEDs that is well-suited to work with "liquid" DNA nanoconstructions as biosensing units. The mobile and inexpensive device is intended for use in a biosensor analytical system for rapid determination of biologically active compounds in liquids to solve practical problems of clinic medicine and pharmacology.We develop optical biosensor analytical systems, in which "liquid" particles of the cholesteric liquid-crystalline dispersion (CLCD) of DNA are used as a biosensing unit, and a portable dichrometer registers changes of the circular dichroism (CD) signal generated with the DNA biosensing unit during its interaction with a defined biologically active compound (BAC) [1]. DNA biosensing units exhibit anomalously large optical activity in the CD spectrum in an absorption band of DNA nitrogen bases (chromophores) at 270 nm, the magnitude of which is stable and acts as a convenient "quality" criterion of prepared DNA CLCD particles. Intercalation of coloured BACs into the DNA cholesteric liquidcrystalline structure leads to the appearance in the CD spectrum of an additional abnormal band in the visible spectrum, whose amplitude depends on the concentration of BAC -this calibration dependence is used then to establish the presence of such compounds in the assay medium and to determine their concentration.Among our developments there are dichrometers with tunable wavelength light sources [2, 3] which, due to their versatility, are primarily used for research purposes. Taking into account the specificity of the CD spectrum generated by biosensing units based on "liquid" DNA CLCD particles at intercalation therein of coloured BAC, the idea has occured to develop a compact biosensor analytical system for the rapid detection in clinical conditions of several important BAC, in particular, antibiotics (daunorubicin and analogues) used in oncological practice. Such the biosensor would work on only two discrete wavelengths, one of which corresponds to the absorption band of DNA chromophores at a wavelength of 270 nm, and the other -to the abnormal band in the visible region of the CD spectrum of the (DNA + BAC) complex. Such a choice predetermined steps to simplification of the dichrometer -a rejection of expensive broadband lamp (Xe) of the radiation source, a monochromator and a photomultiplier. The use of modern high power light-emitting diodes as light sources and a photodiode for registering the CD signal allows both DNA CLCD particles quality control at a wavelength of 270 nm and registration of the smallest changes

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New optical evidence of the cholesteric packing of DNA molecules in “re-entrant” phase

Yevdokimov

Skuridin

Salyanov

et al. 2019

Chemical Physics Letters

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Nanostructures and Nanoconstructions based on DNA

Cited by 13 publications

References 2 publications

The Physicochemical and Nanotechnological Approaches to Creation of “Rigid” DNA Nanoconstructions

The Physicochemical and Nanotechnological Approaches to Creation of “Rigid” DNA Nanoconstructions

A compact two-wave dichrometer of an optical biosensor analytical system for medicine

New optical evidence of the cholesteric packing of DNA molecules in “re-entrant” phase

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