Nanostructured Systems Containing Rutin: In Vitro Antioxidant Activity and Photostability Studies

Abstract

The improvement of the rutin photostability and its prolonged in vitro antioxidant activity were studied by means of its association with nanostructured aqueous dispersions. Rutin-loaded nanocapsules and rutin-loaded nanoemulsion showed mean particle size of 124.30 ± 2.06 and 124.17 ± 1.79, respectively, polydispersity index below 0.20, negative zeta potential, and encapsulation efficiency close to 100%. The in vitro antioxidant activity was evaluated by the formation of free radical ·OH after the exposure of hydrogen peroxide to a UV irradiation system. Rutin-loaded nanostructures showed lower rutin decay rates [(6.1 ± 0.6) 10−3 and (5.1 ± 0.4) 10−3 for nanocapsules and nanoemulsion, respectively] compared to the ethanolic solution [(35.0 ± 3.7) 10−3 min−1] and exposed solution [(40.1 ± 1.7) 10−3 min−1] as well as compared to exposed nanostructured dispersions [(19.5 ± 0.5) 10−3 and (26.6 ± 2.6) 10−3, for nanocapsules and nanoemulsion, respectively]. The presence of the polymeric layer in nanocapsules was fundamental to obtain a prolonged antioxidant activity, even if the mathematical modeling of the in vitro release profiles showed high adsorption of rutin to the particle/droplet surface for both formulations. Rutin-loaded nanostructures represent alternatives to the development of innovative nanomedicines.

Controlled release of raloxifene by nanoencapsulation: effect on in vitro antiproliferative activity of human breast cancer cells

“…The zeta potential was analyzed by electrophoretic mobility using the same instrument, after dilution of the formulations in 10 mM NaCl aqueous solution (1:500 volume per volume). 24 Nanocapsules tracking analysis (NTA; NanoSight LM10 and NTA Analytical Software, Amesbury, UK) was used to estimate the particle size distribution, the number of particles per milliliter, and the presence of nanocrystals after appropriate dilution of the nanocapsule suspensions in purified water (10,000× and 5,000× for Eudragit ® RS100 and Eudragit ® S100 nanocapsule suspensions, respectively). Furthermore, this analysis allowed the observation of the Brownian motion of the individual nanoparticles.…”

“…24,27 The model that best describes the drug release profile was selected through the model selection criteria (MSC), graphic adjustment, and the correlation coefficient. In order to obtain an additional parameter to compare the drug release behavior between the formulations, half-lives were calculated according to equation 1:…”

“…On the other hand, the RH-NC-S released only 51.97%±1.10% of the drug after the same length of time, showing the best drug release control. 24,[27][28][29]33 For a deeper evaluation of the influence of the surface charge on the RH release profiles, whether positive (RH-NC-RS) or negative (RH-NC-S), the nanocapsule suspensions were modeled according to the mono and biexponential models.…”

Sistemas Nanoestruturados Contendo Óleo De Linhaça: Desenvolvimento Tecnológico E Caracterização Físico-Química De Nanoemulsões E Nanocápsulas Poliméricas

“…Na literatura são encontrados trabalhos que mostram a associação de diferentes óleos a nanoestruturas de PCL como polímero nos quais também foram avaliados o tamanho das partículas em suspensão 20,33,34 . Em outro trabalho no qual foram desenvolvidas suspensões de nanocápsulas também pelo método de deposição interfacial do polímero PCL, observou-se partículas com tamanhos menores que 270 nm, valores estes bastante semelhantes aos encontrados neste estudo para a formulação NC-PCL-B 30 .…”

Determination of Methotrexate in pH-Sensitive Chitosan Nanoparticles by Validated RP-LC and UV Spectrophotometric Methods.

“…The graph with the best fit was then considered to establish the kinetic order. Finally, halflife (t 1/2 ) and t 90 value (time for 10% decomposition) were determined from the k-value [24].…”