Nanostructured phospholipid membranes

Badia, Antonella; Moraille, Patricia; Tang, Nathalie; Randlett, Marie Eve

doi:10.1504/ijnt.2008.019842

Cited by 14 publications

(24 citation statements)

References 98 publications

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“…Both BAM (Figures a–a) and AFM (Figures a and a) imaging show that upon compression on ultrapure water the lipid-only mixtures and Infasurf laterally phase separate into thicker DPPC-rich condensed domains and a thinner LE phase enriched in the low T m saturated and/or unsaturated lipid(s) and surfactant proteins in the case of Infasurf. AFM analyses reveal apparent height differences ranging from 0.5 to 1 nm (depending on the force applied to the sample) between the condensed and LE monolayer phases that are a convolution of the true monolayer thickness and the phase compliance . The AFM tip–sample adhesion is larger over the LE versus condensed phase (Figures S2 and S3), reflecting a difference in the mechanical properties and possibly the charge (electrostatic forces) in the case of DPPC/POPG and Infasurf …”

Section: Resultsmentioning

confidence: 99%

“…AFM analyses reveal apparent height differences ranging from 0.5 to 1 nm (depending on the force applied to the sample) between the condensed and LE monolayer phases that are a convolution of the true monolayer thickness and the phase compliance. 48 The AFM tip−sample adhesion is larger over the LE versus condensed phase (Figures S2 and S3), reflecting a difference in the mechanical properties and possibly the charge (electrostatic forces) in the case of DPPC/POPG and Infasurf. 49 Binary Lipid Mixtures.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Silica Nanoparticle-Induced Structural Reorganizations in Pulmonary Surfactant Films: What Monolayer Compression Isotherms Do Not Say

Borozenko

Faral²,

Behyan

et al. 2018

ACS Appl. Nano Mater.

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The interaction of nanoparticles (NPs) with pulmonary surfactant is important for understanding the potential adverse effects of inhaled engineered and incidental nanomaterials. The effects of a low concentration (0.001 wt %) of charged, hydrophilic silica NPs of hydrodynamic diameter of ∼20 nm on the phase behavior and lateral structure of lipid-only and naturally derived surfactant monolayers were investigated at the air/water interface using surface pressure–area isotherms and Brewster angle microscopy, respectively. Atomic force microscopy was used to image the morphology of films transferred onto mica substrate with nanometer resolution. We show that the silica NPs can significantly alter the condensed domain size and shape even in the absence of apparent differences in the monolayer compression isotherms. The cationic particles notably induce structural and morphological progressions in a binary model containing anionic phosphoglycerol that are similar to those observed for the natural surfactant film that contains cationic proteins. These findings specifically highlight the impact of the NP charge on the phase transformations in pulmonary surfactant, with implications for the engineering of nanomaterials for commercial use and bioapplications.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Silica Nanoparticle-Induced Structural Reorganizations in Pulmonary Surfactant Films: What Monolayer Compression Isotherms Do Not Say

Borozenko

Faral²,

Behyan

et al. 2018

ACS Appl. Nano Mater.

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“…Vie et al 34 found a similar feature of the DPPC-DOPC mixed monolayer on a mica surface deposited at 37 mN m À1 . Moraille and Badia 35,36 reported the formation of stripe pattern structures of DPPC and 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) mixed monolayers and bilayers on a mica surface under different conditions. The height difference between the two domains in Fig.…”

Section: Monolayer Stability In Different Environmentsmentioning

confidence: 99%

Structure and stability studies of mixed monolayers of saturated and unsaturated phospholipids under low-level ozone

Qiao

Osawa

et al. 2013

Phys. Chem. Chem. Phys.

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In the present study, stability and structure of single and binary mixed monolayers of an unsaturated phospholipid, DOPC, and a saturated phospholipid, DPPC-d75, on the water surface, were explored using the π-A isotherm, atomic force microscopy (AFM) and sum frequency generation (SFG) vibrational spectroscopy in various environments. Our results demonstrated that DOPC in the monolayers becomes unstable after the exposure to a low concentration of ozone (20 ± 10 ppb) or even to ambient laboratory air, which has a similar ozone level, but is stable in nitrogen or oxygen. DOPC can be selectively oxidized by a trace amount of ozone in the ambient environment but can be partially inhibited by the presence of DPPC in the monolayer. The present study provides useful information for understanding the physicochemical properties of the cell membranes.

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“…Two traditional methods for the deposition of single or multiple lipid bilayers are Langmuir-Blodgett (Fig. 1c-d), 46,98,99 and Langmuir-Schaefer deposition. 100 Literature describing the use of these methods is abundant, and both methods are well-established.…”

Section: 21mentioning

confidence: 99%

Molecular phospholipid films on solid supports

2011

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Phospholipid membranes are versatile structures for mimicking biological surfaces. Bilayer and monolayer membranes can be formed on solid supports, leading to enhanced stability and accessibility of the biomimetic molecular film. This has facilitated functional studies of membrane proteins and aided the development of membrane-based applications in, for example, biosensing, self-assembled reaction kinetics and catalysis. Assembly and preparation of lipid films on supporting surfaces is a challenging engineering task with the goal of fabricating mechanically, chemically and thermodynamically stable lipid membranes. In this review, the current state of the art of molecularly thin lipid layer fabrication is presented with an emphasis on support materials, film formation mechanisms, characterisation methods, and applications.

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Nanostructured phospholipid membranes

Cited by 14 publications

References 98 publications

Silica Nanoparticle-Induced Structural Reorganizations in Pulmonary Surfactant Films: What Monolayer Compression Isotherms Do Not Say

Silica Nanoparticle-Induced Structural Reorganizations in Pulmonary Surfactant Films: What Monolayer Compression Isotherms Do Not Say

Structure and stability studies of mixed monolayers of saturated and unsaturated phospholipids under low-level ozone

Molecular phospholipid films on solid supports

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