Nanostructured Metal-Enhanced Raman Spectroscopy for DNA Base Detection

Zhu, Zhiqian; Zhan, Li; Hou, Cailing; Wang, Zhimin

doi:10.1109/jphot.2012.2208945

Cited by 8 publications

(3 citation statements)

References 23 publications

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“…Nanophotonic sensing methods based on the SERS detection of DNA, RNA, and protein sequences on solid-state and biological platforms [1,2] have reached single nucleotide or amino acid sensitivity [1][2][3][4][5][6][7][8][9][10][11][12][13]; however, a number of challenges still remain ahead of technological implementation. High sensitivity and selectivity in small volumes have been achieved by the manipulation of plasmonic enhancers used to create hot spots for the measured molecules by using different shapes, assays, and surfaces of gold or silver nanostructures [12,13].…”

Section: Introductionmentioning

confidence: 99%

MD Average of Vibrational Spectra of Nucleotides in a SERS Sensor Simulation with Varying Number of Au Nanoparticles

Zolotoukhina

Maruyama

2023

Iecb 2023

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Section: Introductionmentioning

confidence: 99%

MD Average of Vibrational Spectra of Nucleotides in a SERS Sensor Simulation with Varying Number of Au Nanoparticles

Zolotoukhina

Maruyama

2023

Iecb 2023

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“…Chemical luminescence studies made molecular recognition and DNA detection simple, sensitive and easy [10]. While Raman spectroscopy aided nucleotide identification and DNA sequencing [11]. Gas chromatography, spectrophotometric methods and high-performance liquid chromatography detected DNA bases in several different sample matrices [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Hybrid Nanomaterial of Graphene Oxide Quantum Dots with Multi-Walled Carbon Nanotubes for Simultaneous Voltammetric Determination of Four DNA Bases

et al. 2023

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In this proof-of-concept study, a novel hybrid nanomaterial-based electrochemical sensor was developed for the simultaneous detection of four DNA bases. For the modification of the working electrode surface, graphene oxide quantum dots (GOQDs) were synthesized using a solvothermal method. GOQDs were then used for the preparation of a hybrid nanomaterial with multi-walled carbon nanotubes (GOQD-MWCNT) using a solvothermal technique for the first time. Transmission electron microscopy (TEM) was used to characterize the GOQDs-MWCNTs. A glassy carbon electrode (GCE) was modified with the GOQDs-MWCNTs using Nafion™ to prepare a GOQD-MWCNT/GCE for the simultaneous determination of four DNA bases in phosphate buffer solution (PBS, pH 7.0) using differential pulse voltammetry (DPV). The calibration plots were linear up to 50, 50, 500, and 500 µM with a limit of detection at 0.44, 0.2, 1.6, and 5.6 µM for guanine (G), adenine (A), thymine (T) and cytosine (C), respectively. The hybrid-modified sensor was used for the determination of G, A, T, and C spiked in the artificial saliva samples with the recovery values ranging from 95.9 to 106.8%. This novel hybrid-modified electrochemical sensor provides a promising platform for the future development of a device for cost-effective and efficient simultaneous detection of DNA bases in real biological and environmental samples.

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“…2,3 Therefore, detection and quantification of nucleobases are essential from the biological and clinical perspective. There are many reports and protocols in the literature that employ strategies such as liquid chromatography, 4 mass spectroscopy, 5 flow-injection chemiluminescence, 6 fluorescence spectroscopy, [7][8][9] high-performance liquid chromatography, 10 capillary electrophoresis, 11 Raman spectroscopy 12 and surfaceenhanced Raman spectroscopy 13 for DNA nucleobases detection. However, these strategies suffer from issues such as cumbersome operating procedures, labor intensive, expensive and not rapid.…”

mentioning

confidence: 99%

Sulfur Doped Graphitic Carbon Nitride Nanosheets for the Electrochemical Detection of DNA Bases

Krishnan,

Sakthivel,

Alwarappan

2023

J. Electrochem. Soc.

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Sulfur doped graphitic carbon nitrate (S-g-C3N4) was prepared by a thermal polymerization method using thiourea as a precursor. The as-prepared S-g-C3N4 was characterized by various surface probing techniques and employed as a platform for the electrochemical detection of DNA bases guanine (G), adenine (A), thymine (T), and cytosine (C). Effect of various buffers and pH essential for the selective and sensitive detection of all the DNA bases were also investigated. From the results, it was evident that PBS (pH 9.0) buffer is the best suitable medium for the DNA detection. A limit of detection of 13.22, 13.1, 37.7, and 14.61 µM (S/N = 3) and sensitivity of 1.76, 2.41, 0.68 and 2.38 µA cm-2 µM-1corresponding to G, A, T and C respectively were noticed. Further, the proposed sensor was found to be linear in the range of 5–35 μM (G), 5–35 μM (A), 25-175 μM (T) and 25–175 μM (C). The proposed S-g-C3N4 modified electrode also exhibited a stable, selective and sensitive performance towards the detection of individual DNA base in the presence of other DNA bases.

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Nanostructured Metal-Enhanced Raman Spectroscopy for DNA Base Detection

Cited by 8 publications

References 23 publications

MD Average of Vibrational Spectra of Nucleotides in a SERS Sensor Simulation with Varying Number of Au Nanoparticles

MD Average of Vibrational Spectra of Nucleotides in a SERS Sensor Simulation with Varying Number of Au Nanoparticles

Hybrid Nanomaterial of Graphene Oxide Quantum Dots with Multi-Walled Carbon Nanotubes for Simultaneous Voltammetric Determination of Four DNA Bases

Sulfur Doped Graphitic Carbon Nitride Nanosheets for the Electrochemical Detection of DNA Bases

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