Nanostructured lipid carriers loaded with CoQ10: Effect on human dermal fibroblasts under normal and UVA-mediated oxidative conditions

Brugè, Francesca; Damiani, Elisabetta; Puglia, Carmelo; Offerta, Alessia; Armeni, Tatiana; Littarru, Gian Paolo; Tiano, Luca

doi:10.1016/j.ijpharm.2013.06.075

Cited by 51 publications

(31 citation statements)

References 43 publications

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“…Increased oxidative stress in aging cells may be ameliorated with dietary supplementation of CoQ 10 [34]. Indeed, CoQ 10 has garnered great popularity as an antioxidant in moisturizers, anti-wrinkle and anti-aging skin care treatments [35–37]. With the growing demand for skin care cosmetics and public awareness of the importance of antioxidants, we will likely see an increase in the demand for CoQ 10 products on the market quite quickly [38].…”

Section: Introductionmentioning

confidence: 99%

Cellular factories for coenzyme Q10 production

et al. 2017

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Coenzyme Q10 (CoQ10), a benzoquinone present in most organisms, plays an important role in the electron-transport chain, and its deficiency is associated with various neuropathies and muscular disorders. CoQ10 is the only lipid-soluble antioxidant found in humans, and for this, it is gaining popularity in the cosmetic and healthcare industries. To meet the growing demand for CoQ10, there has been considerable interest in ways to enhance its production, the most effective of which remains microbial fermentation. Previous attempts to increase CoQ10 production to an industrial scale have thus far conformed to the strategies used in typical metabolic engineering endeavors. However, the emergence of new tools in the expanding field of synthetic biology has provided a suite of possibilities that extend beyond the traditional modes of metabolic engineering. In this review, we cover the various strategies currently undertaken to upscale CoQ10 production, and discuss some of the potential novel areas for future research.

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Section: Introductionmentioning

confidence: 99%

Cellular factories for coenzyme Q10 production

et al. 2017

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“…This proliferative effect of all formulations was found to be statistically significant. Comparing the two formulations of BMV and DFV, liposome formulations appeared to have higher proliferative effect then their nanoparticles (Brugè et al, 2013).…”

Section: Cell Culture Studiesmentioning

confidence: 90%

Effective topical delivery systems for corticosteroids: dermatological and histological evaluations

et al. 2014

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Atopic dermatitis (AD) is a chronic and relapsing skin disease with severe eczematous lesions. Long-term topical corticosteroid treatment can induce skin atrophy, hypopigmentation and transepidermal water loss (TEWL) increase. A new treatment approach was needed to reduce the risk by dermal targeting. For this purpose, Betamethasone valerate (BMV)/Diflucortolone valerate (DFV)-loaded liposomes (220-350 nm) were prepared and incorporated into chitosan gel to obtain adequate viscosity ($13 000 cps). Drugs were localized in stratum corneum + epidermis of rat skin in ex-vivo permeation studies. The toxicity was assessed on human fibroblast cells. In point of in-vivo studies, pharmacodynamic responses, treatment efficacy and skin irritation were evaluated and compared with previously prepared nanoparticles. Liposome/ nanoparticle in gel formulations produced higher paw edema inhibition in rats with respect to the commercial cream. Similar skin blanching effect with commercial creams was obtained via liposome in gels although they contain 10 times less drug. Dermatological scoring results, prognostic histological parameters and suppression of mast cell numbers showed higher treatment efficiency of liposome/nanoparticle in gel formulations in AD-induced rats. TEWL and erythema measurements confirmed these results. Overview of obtained results showed that liposomes might be an effective and safe carrier for corticosteroids in skin disease treatment.

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“…NLC's were prepared by hot high‐speed homogenization method . In brief, the components of the lipid phase as described in Table were mixed and heated at 80 °C until it was totally melted . The aqueous phase containing tween 80 was simultaneously prepared at the same temperature.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic Anti-Inflammatory Potential of Different Formulations Based on Coenzyme Q10-Loaded Nanostructured Lipid Carrier: In Vitro, Ex Vivo, and In Vivo Evaluations

Ahmadi¹,

Rostamizadeh

Modarresi‐Alam³

2018

Eur. J. Lipid Sci. Technol.

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An attempt is made to study skin anti‐inflammatory activity for different formulations of Co‐Q10 loaded nanostructured lipid carriers (NLC) including NLC, NLC‐based gel, and W/O cream. NLCs are prepared by a hot high speed homogenization method using cetylpalmitate as solid lipid, coconut oil as liquid lipid, and tween 80 as surfactant. NLCs are characterized by different techniques including dynamic light scattering, transmission electron microscopy, and FT‐IR. Stable formulation with a mean size range of 118.7 ± 6.9 nm and zeta potential of −29.1 ± 1.96 mV is developed. Drug loading content for NLC/Co‐Q10, NLC‐based gel, and W/O cream formulations is 7.945 ± 0.035%, 4.79 ± 0.052%, and 2.39 ±0.041%, respectively. All formulations show high entrapment efficiency (EE) (>96% w/w). The formulations are prepared and characterized in terms of rheology, pH, anti‐radical activity, ex vivo skin permeation, and in vivo anti‐inflammatory activity. A drug release study exhibits that the maximum attainable drug release after 48 h for NLC/Co‐Q10 is 11.94% versus 14.4% for NLC‐based gel and 41.56% for W/O cream. Ex vivo studies on rat skin reveals that Co‐Q10 contained in the W/O cream formulation penetrates into the stratum corneum more readily than that in the NLC/Co‐Q10 and NLC‐based gel formulations. In vivo studies of acute anti‐inflammatory activity of NLC‐based formulations show significant difference between inhibition effect of W/O cream and NLC/Co‐Q10 compared to betamethasone. Practical Applications: The findings indicate that formulation based on NLC/Co‐Q10 is suitable not only in skin penetration but also in dermatology. Anti‐inflammatory activity for different formulations of Co‐Q10 loaded NLC including NLC, NLC‐based gel, and W/O cream is investigated. All formulations show suitable physicochemical properties and high drug loading capacity for being used as dermatological formulations. The order of drug release after 48 h is NLC/Co‐Q10 < NLC‐based gel < W/O cream. In ex vivo study, the fastest penetration rate into the stratum corneum is obtained for W/O cream. W/O cream and NLC/Co‐Q10 formulations show significant acute anti‐inflammatory activity compared to betamethasone in in vivo study.

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Nanostructured lipid carriers loaded with CoQ10: Effect on human dermal fibroblasts under normal and UVA-mediated oxidative conditions

Cited by 51 publications

References 43 publications

Cellular factories for coenzyme Q10 production

Cellular factories for coenzyme Q10 production

Effective topical delivery systems for corticosteroids: dermatological and histological evaluations

Therapeutic Anti-Inflammatory Potential of Different Formulations Based on Coenzyme Q10-Loaded Nanostructured Lipid Carrier: In Vitro, Ex Vivo, and In Vivo Evaluations

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