Nanostructured Films as a Novel Substrate for Chondrocytes Growth

Abstract

We fabricated a large area silica nano-particle monolayer on glass substrates for the cell growth by the Langmuir-Blodgett technique. A thin film of 300 nm sized mono-dispersed silica particles was constructed on the air-water interface and transferred onto a glass substrate. Chondrocytes were cultured on nano-structured substrates and bare glass substrates for 8 days. The characterizations of chondrocytes on nano-structured substrate were conducted on 3rd and 6th day using confocal laser microscopy and with MTT assay for 8 days. The chondrocytes cultured on nano-structured substrate showed podia like spike and their size was larger than that formed on bare glass substrate. The metabolic activity of chondrocytes on nano-structured substrate was lower than that on bare glass substrate at early-stage, but it was recovered after 4 days.

Behavior of Muscle-Derived Stem Cells on Silica Nanostructured Substrates

“…However, because the silica substrates reported to date have relatively limited biocompatibility, there is a lack of understanding of the cellular responses of mammalian cells to growth on silica substrates [ 14 , 15 ]. In previous studies, our group fabricated silica nanostructured substrates (SN) on glass using the LB technique, demonstrating that this approach provides a simple method for producing uniform and reproducible SN with nanoscale level roughness [ 16 , 17 , 18 ]. We also examined SN as culture substrates for chondrocytes and observed that SN promoted good adhesion and proliferation without promoting dedifferentiation and thus served as adaptable culture substrates for chondrocytes [ 18 ].…”

“…In previous studies, our group fabricated silica nanostructured substrates (SN) on glass using the LB technique, demonstrating that this approach provides a simple method for producing uniform and reproducible SN with nanoscale level roughness [ 16 , 17 , 18 ]. We also examined SN as culture substrates for chondrocytes and observed that SN promoted good adhesion and proliferation without promoting dedifferentiation and thus served as adaptable culture substrates for chondrocytes [ 18 ]. Although chondrocytes were found to adhere, spread, and proliferate on SN, additional detailed studies of cellular responses of other mammalian cells grown on SN are needed.…”