NanoSMGT: Transgene transmission into bovine embryos using halloysite clay nanotubes or nanopolymer to improve transfection efficiency

Campos, Vinícius Farias; Leon, Priscila Marques Moura de; Komninou, Eliza Rossi; Dellagostin, Odir A.; Deschamps, João Carlos; Seixas, Fabiana Kömmling; Collares, Tiago

doi:10.1016/j.theriogenology.2011.06.027

Cited by 46 publications

(32 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fi rst report that exogenous DNA could be introduced into sperm was made by Brackett et al (1971). Several studies in distinct species have reported the generation of transgenic animals using spermatozoa as vectors to carrier foreign DNA to the ova (Lu et al, 2002;Webster et al, 2005;Shen et al, 2006;Hoelker et al, 2007, Campos et al, 2011a, Campos et al, 2011b, Campos et al, 2011c. One approach of sperm-mediated gene transfer (SMGT) is the direct introduction of foreign DNA into testes, so-called testis-mediated gene transfer (TMGT), which allows for natural mating and mass gene transfer.…”

Section: Introductionmentioning

confidence: 99%

Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage

et al. 2011

Self Cite

View full text Add to dashboard Cite

Testis-mediated gene transfer (TMGT) has been used as in vivo gene transfer technology to introduce foreign DNA directly into testes, allowing mass gene transfer to offspring via mating. In this study, we used plasmid DNA (pEGFP-N1) mixed with dimethylsulfoxide (DMSO), N,N-dimethylacetamide (DMA) or liposome (Lipofectin) in an attempt to improve TMGT. Males receiving consecutive DNA complex injections were mated to normal females to obtain F0 progeny. In vivo evaluation of EGFP expression, RT-PCR and PCR were used to detect the expression and the presence of exogenous DNA in the progeny. We also evaluated possible testicular damage by histological procedures. PCR and RT-PCR analyses revealed that liposome and DMSO increased the rate of TMGT. Histological analyses demonstrated that repeated (4 times) injections of DNA complexes can affect spermatogenesis. DMSO was the most deleterious among the reagents tested. In this study, we detected the presence of transgene in the progeny, and its expression in blood cells. Consecutive injections of DNA complexes were associated with impaired spermatogenesis, suggesting requirement of optimal conditions for DNA delivery through TMGT.

show abstract

Section: Introductionmentioning

confidence: 99%

Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recent advances in the use of nanocarriers for gene transfer into reproductive tissues, gametes, and embryos are rapidly creating a powerful tool with which to manipulate and study pathophysiological mechanisms in the reproductive sciences. [25][26][27] For example, our group has recently demonstrated the ability of MSNPs to bind with mammalian sperm without negative effects upon key semen parameters. 22 In addition, customization of the aforementioned nanoparticles with a cell-penetrating peptide coating has been shown to increase the rates of association with sperm.…”

Section: Discussionmentioning

confidence: 99%

Development of a laser-activated mesoporous silica nanocarrier delivery system for applications in molecular and genetic research

et al. 2016

View full text Add to dashboard Cite

Coward, "Development of a laseractivated mesoporous silica nanocarrier delivery system for applications in molecular and genetic research," J. Biomed. Opt. 21(11), 115002 (2016), doi: 10.1117/1.JBO.21.11.115002. Abstract. Nanoparticles have revolutionized medical research over the last decade. One notable emerging area of nanomedicine is research developments in the reproductive sciences. Since increasing evidence indicates links between abnormal gene expression and previously unexplained states of infertility, there is a strong impetus to develop tools, such as nanoparticle platforms, to elucidate the pathophysiological mechanisms underlying such states. Mesoporous silica nanoparticles (MSNPs) represent a powerful and safe delivery tool for molecular and genetic investigations. Nevertheless, ongoing progress is restricted by low efficiency and unpredictable control of cargo delivery. Here, we describe for the first time, the development of a laser-activated MSNP system with heat-responsive cargo. Data derived from human embryonic kidney cells (HEK293T) indicate that when driven by a heat-shock promoter, MSNP cargo exhibits a significantly increased expression following infrared laser stimulus to stimulate a heat-shock response, without adverse cytotoxic effects. This delivery platform, with increased efficiency and the ability to impart spatial and temporal control, is highly useful for molecular and genetic investigations. We envision that this straightforward stimuli-responsive system could play a significant role in developing efficient nanodevices for research applications, for example in reproductive medicine. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

“…Thereby they serve as natural vectors for transfection of the oocyte and, consequently, of a developing embryo via IVF (Campos et al, 2011b). This treatment did not affect sperm motility or viability, but both Xand Y-sorted sperm had decreased DNA uptake in comparison with unsorted sperm (Campos et al, 2011a). Constructs of NPs conjugated with exogenous DNA are successfully transferred via SMGT into fertilized oocytes, but expression of the assayed protein in morulae and blastocysts is not always detectible Campos et al, 2011a).…”

Section: Embryogenesismentioning

confidence: 99%

“…Some researchers have demonstrated biocompatibility with mammalian sperm for magnetic iron NPs , mesoporous silica NPs (Barkalina et al, 2014b), halloysite clay nanotubes and commercial nanopolymer-based transfectants (Campos et al, 2011a). In turn, alternative data regarding the reproductive toxicity of most commonly used nanostructures, such as AuNPs, AgNPs and titanium dioxide (TiO 2 ) NPs, remain highly contradictory.…”

Section: Risk Of the Use Of Nanomaterialsmentioning

confidence: 99%

Nanobiotechnology in reproduction – pros and cons. A review

Zielińska‐Górska

Górski

2015

J. Anim. Feed Sci.

View full text Add to dashboard Cite

NanoSMGT: Transgene transmission into bovine embryos using halloysite clay nanotubes or nanopolymer to improve transfection efficiency

Cited by 46 publications

References 38 publications

Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage

Testis-mediated gene transfer in mice: comparison of transfection reagents regarding transgene transmission and testicular damage

Development of a laser-activated mesoporous silica nanocarrier delivery system for applications in molecular and genetic research

Nanobiotechnology in reproduction – pros and cons. A review

Contact Info

Product

Resources

About