NanoSIMS50 analyses of Ar/¹⁸O₂plasma-treatedEscherichia colibacteria

Evidence of radicals created by plasma in bacteria in water

“…Plasma is generated in the porous alumina between the electrodes which are separated by a gap of 1 mm. 18 The dielectric alumina used in this work has approximately 31 vol. % porosity, and the average pore diameter is 100 lm (MP100, Mitsui).…”

Marangoni flows induced by atmospheric-pressure plasma jets

“…Atmospheric-pressure plasma jets [1,2] have generated significant interest for their versatile applications in material processing [1][2][3][4], surface modification [5][6][7][8], medicine [9][10][11][12][13][14][15][16][17][18][19][20][21] and chemical reaction engineering [22][23][24][25]. Fricke et al studied the etching of polymers using atmosphericpressure Ar plasma jets with different oxygen admixtures and found etchrates in the order of 20 nm s −1 for polyethylene [4].…”

Study of Reactive Oxygen or/and Nitrogen Species Binding Processes on E. coli Bacteria with Mass Spectrometry Isotopic Nanoimaging

“…In order to transfer these efficient methods to the healthcare industry and to other sectors as the food industry, research teams must now better understand the plasma‐biological media interaction and eventual side effects induced by plasma treatments on living tissues and organisms, so that the cure would not be worse than the disease. This can be done by characterizing more precisely the plasmas used for such applications, by better understanding the interactions of these plasmas with the environment of the biological tissues or microorganisms (water, culture media, biological liquids,…) comparing them with non‐plasma treatments effects (e.g., UV irradiation alone), and by improving the characterization of the treated microorganisms and biological tissues . This work focuses on the last issue with the aim of proposing a new analysis method to study the effects of plasma treatments on microorganisms.…”

“…The aim is here to present a novel methodology to assess single‐cell chemical modifications on plasma treated Escherichia coli bacteria, and which will be applicable to all microorganisms treated at low or atmospheric pressure in the future. In a previous paper, secondary ion mass spectrometry performed on bacteria treated by a plasma containing isotopic 18 O 2 , revealed how reactive oxygen species (ROS) affect the living organism. It was possible to image parts where ROS bind with the bacterial structures by observing cross‐sections of the treated bacteria and to give a rough estimate of ROS found, respectively, on the surface of the bacteria and inside .…”

“…In a previous paper, secondary ion mass spectrometry performed on bacteria treated by a plasma containing isotopic 18 O 2 , revealed how reactive oxygen species (ROS) affect the living organism. It was possible to image parts where ROS bind with the bacterial structures by observing cross‐sections of the treated bacteria and to give a rough estimate of ROS found, respectively, on the surface of the bacteria and inside . This new method was shown to be very useful and complementary to other characterization methods (electronic microscopies, enumeration of survivor bacteria, epifluorescence microscopy, mutation assessment of DNA and others).…”