Nanosilver Particle Effects on Drug Metabolism In Vitro

Lamb, John G.; Hathaway, Laura B.; Munger, Mark A.; Raucy, Judy L.; Franklin, Michael R.

doi:10.1124/dmd.110.035238

Cited by 37 publications

(36 citation statements)

References 17 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, AgNPs are well known for their antimicrobial properties, used extensively in a range of medical and general applications [3]. Henceforth, the toxicity of AgNPs has been studied widely in many in vitro [4][5][6] and in vivo models [1,7,8]. Recent studies have demonstrated the adverse effects of AgNPs on the male reproductive tract, particularly spermatogenesis and the quality of sperm and male somatic cells and spermatogonial stem cells [9,10].…”

Section: Introductionmentioning

confidence: 99%

Sodium Selenite Protects Against Silver Nanoparticle-Induced Testicular Toxicity and Inflammation

Ansar

Abudawood

Hamed

et al. 2016

Biol Trace Elem Res

View full text Add to dashboard Cite

Metal nanomaterials hold great potential and play an important role in consumer products. However, the increasing use of nanomaterials has raised concern over inadvertent exposure and potential risks for human health and the environment. Henceforth, in vivo testing of nanoparticles and protection against its toxicity is required. Using rat as an animal model, effect of sodium selenite (Se), an essential trace element, on rat testes exposed to silver nanoparticles (AgNPs) was evaluated. Male rats were treated with AgNPs (5 mg/kg/b.w) i/p or Se (0.2 mg/kg/b.w) by gavage. AgNP administration decreased Glutathione (GSH) levels and activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) and increased levels of malondialdehyde (MDA) and expression of interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α). However, treatment with Se increased GSH levels and activities of SOD, CAT, and GPx compared with AgNP-treated group and decreased the level of MDA and inflammatory biomarkers significantly (p < 0.05) as compared with AgNP-treated group. Light microscopic analyses also revealed that AgNP induced histopathological changes in testes tissue. Further, protection by Se on biochemical results was confirmed by alleviation of the histopathological changes in the tissue. Results show the adverse effects of AgNPs on the male reproductive tract, particularly spermatogenesis, and suggest that Se possesses significant potential in reducing AgNP-induced testicular toxicity.

show abstract

Section: Introductionmentioning

confidence: 99%

Sodium Selenite Protects Against Silver Nanoparticle-Induced Testicular Toxicity and Inflammation

Ansar

Abudawood

Hamed

et al. 2016

Biol Trace Elem Res

View full text Add to dashboard Cite

show abstract

“…DPX2 cells (HepG2 background with human PXR stably overexpressed to drive the expression of a CYP3A4 reporter gene construct by PXR agonists) were provided by Dr. Judy Raucy (Puracyp Inc., Carlsbad, CA). DPX2 cells were cultured in Puracyp media (Puracyp Inc.) (Lamb et al, 2010). Both cell lines were plated in six-well plates and treated with BDP (10 mM) in Opti-MEM 1 reduced serum media (Life Technologies) at 70% confluence, with and without esterase inhibitors (paraoxon and eserine 1:1; 175 mM), for 24 hours.…”

Section: Methodsmentioning

confidence: 99%

Metabolism of Beclomethasone Dipropionate by Cytochrome P450 3A Enzymes

Roberts¹,

Moore²,

Ward³

et al. 2013

J Pharmacol Exp Ther

View full text Add to dashboard Cite

Inhaled glucocorticoids, such as beclomethasone dipropionate (BDP), are the mainstay treatment of asthma. However, ∼30% of patients exhibit little to no benefit from treatment. It has been postulated that glucocorticoid resistance, or insensitivity, is attributable to individual differences in glucocorticoid receptormediated processes. It is possible that variations in cytochrome P450 3A enzyme-mediated metabolism of BDP may contribute to this phenomenon. This hypothesis was explored by evaluating the contributions of CYP3A4, 3A5, 3A7, and esterase enzymes in the metabolism of BDP in vitro and relating metabolism to changes in CYP3A enzyme mRNA expression via the glucocorticoid receptor in lung and liver cells. . These studies show that CYP3A4 and CYP3A5 metabolize BDP to inactive metabolites and suggest that differences in the expression or function of these enzymes in the lung and/or liver could influence BDP disposition in humans.

show abstract

“…Substrates selective for their respective CYPs are as follows: methoxyresorufin (MR), CYP1A2; 7-benzyloxyquinoline (7-BQ), CYP3A4; and 7-methoxy-4-(aminomethyl)-coumarin (MAMC), CYP2D6. NADPH was added in excess (60 mM) and the formation of fluorescent product was measured using a Biotek-Synergy 2 Microplate Reader [20]. Extracts showing >50% inhibition at K m and/or V max⁡ concentrations were deemed inhibitory (moderate to strong inhibitors as defined by FDA, 2012 [21]).…”

Section: Methodsmentioning

confidence: 99%

Traditional Preparations and Methanol Extracts of Medicinal Plants from Papua New Guinea Exhibit Similar Cytochrome P450 Inhibition

Larson

Pond

Rai

et al. 2016

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

The hypothesis underlying this current work is that fresh juice expressed from Papua New Guinea (PNG) medicinal plants (succus) will inhibit human Cytochrome P450s (CYPs). The CYP inhibitory activity identified in fresh material was compared with inhibition in methanol extracts of dried material. Succus is the most common method of traditional medicine (TM) preparation for consumption in PNG. There is increasing concern that TMs might antagonize or complicate drug therapy. We have previously shown that methanol extracts of commonly consumed PNG medicinal plants are able to induce and/or inhibit human CYPs in vitro. In this current work plant succus was prepared from fresh plant leaves. Inhibition of three major CYPs was determined using human liver microsomes and enzyme-selective model substrates. Of 15 species tested, succus from 6/15 was found to inhibit CYP1A2, 7/15 inhibited CYP3A4, and 4/15 inhibited CYP2D6. Chi-squared tests determined differences in inhibitory activity between succus and methanol preparations. Over 80% agreement was found. Thus, fresh juice from PNG medicinal plants does exhibit the potential to complicate drug therapy in at risk populations. Further, the general reproducibility of these findings suggests that methanol extraction of dried material is a reasonable surrogate preparation method for fresh plant samples.

show abstract

Nanosilver Particle Effects on Drug Metabolism In Vitro

Cited by 37 publications

References 17 publications

Sodium Selenite Protects Against Silver Nanoparticle-Induced Testicular Toxicity and Inflammation

Sodium Selenite Protects Against Silver Nanoparticle-Induced Testicular Toxicity and Inflammation

Metabolism of Beclomethasone Dipropionate by Cytochrome P450 3A Enzymes

Traditional Preparations and Methanol Extracts of Medicinal Plants from Papua New Guinea Exhibit Similar Cytochrome P450 Inhibition

Contact Info

Product

Resources

About