Nanosilicate embedded agarose hydrogels with improved bioactivity

Topuz, Fuat; Nadernezhad, Ali; Çalışkan, Özüm Şehnaz; Menceloǵlu, Yusuf́ Z.; Koç, Bahattin

doi:10.1016/j.carbpol.2018.08.032

Cited by 45 publications

(33 citation statements)

References 35 publications

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“…The degradation of LAP nanoplatelets in the physiological environment leads to the release of products with biological functions. Silica (SiO 2 ) released from in LAP nanoplatelets provided the cellular attachment sites and aided the cell spreading and proliferation 28 . Orthosilicic acid (Si[OH] 4 ) improved collagen type I production and osteodifferentiation in human osteosarcoma cells 29 .…”

Section: Resultsmentioning

confidence: 99%

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Arab‐Ahmadi

Irani

Bakhshi

et al. 2020

Polymers for Advanced Techs

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The side effects and high cost of growth factors and drugs in bone tissue engineering have led to outstanding investigations of alternative osteoinductive supplements. Herein, the electrospun polycaprolactone (PCL) nanofibers were immobilized with carboxymethyl chitosan (CMC) and laponite nanoplatelets (LAP, 0.5‐3.5 wt%) to fabricate osteoinductive scaffolds for bone tissue engineering. The Fourier‐transform infrared)FTIR(and energy dispersive X‐ray (EDX) spectroscopy confirmed the chemical immobilization of CMC and LAP on the surface of PCL nanofibers. Water contact angle measurements exhibited significant improvement of surface hydrophilicity for immobilized scaffolds (<10°) comparing to the virgin PCL nanofibers (125°). The immobilization of CMC and LAP enhanced the attachment, proliferation, and osteodifferentiation of the seeded human bone marrow mesenchymal stem cells (hBM‐MSCs), as evidenced by increased calcium deposition, alkaline phosphatase (ALP) activity, and Osteonectin gene expression. Therefore, the fabricated scaffolds can serve as an appropriate substrate to support the proliferation and differentiation of stem cells to osteoplasts without any external osteoinductive stimulation.

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Section: Resultsmentioning

confidence: 99%

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Arab‐Ahmadi

Irani

Bakhshi

et al. 2020

Polymers for Advanced Techs

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“…In the work by Moxon et al. [6] , an agarose (a widely used polymeric biomaterial that can form an inert hydrogel network widely used for cell encapsulation [20,21]) fluid gel support bath was used for printing. The fluid gel was able, at the same time, to support the extruded GG bioink before crosslinking and allow the free movement of the printing nozzle by self-healing immediately after printing.…”

Section: Introductionmentioning

confidence: 99%

Printing bone in a gel: using nanocomposite bioink to print functionalised bone scaffolds

Cidonio

Cooke

Glinka

et al. 2019

Materials Today Bio

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Free-form printing offers a novel biofabrication approach to generate complex shapes by depositing hydrogel materials within a temporary supportive environment. However, printed hydrogels typically lack the requisite mechanical properties and functionality of the desired tissue, limiting application and, more importantly, safety and efficacy of the implant. The study authors have developed an innovative nanoclay-based bioink to print high shape fidelity functional constructs for potential skeletal application. Laponite® (LAP) nanoclay was combined with gellan gum (GG) to generate a printable hydrogel that was highly stable in vitro, displayed limited swelling ability compared with the silicate-free control and remained stable over time. An agarose fluid gel was found to provide the requisite support for the deposition of the material ink and preservation of the printed structure before crosslinking. Printed C2C12 myoblasts remained viable and displayed extensive proliferation over 21 days in culture. Cell-laden scaffolds demonstrated functionality within 1 day of culture in vitro and that was preserved over 3 weeks. Analysis of absorption and release mechanisms from LAP-GG using model proteins (lysozyme and bovine serum albumin) demonstrated the retention capability of the clay-based materials for compound localisation and absence of burst release. Vascular endothelial growth factor was loaded within the agarose fluid gel and absorbed by the material ink via absorption during deposition. The 3D-printed constructs were implanted on the chorioallantoic membrane of a 10-day-old developing chick. Extensive and preferential vasculature infiltration was observed in LAP-GG–loaded vascular endothelial growth factor constructs compared with controls (p<0.01 and p<0.0001) after only 7 days of incubation. The current studies demonstrate, for the first time, the application of innovative LAP-GG 3D constructs in the generation of growth factor–loaded 3D constructs for potential application in skeletal tissue repair.

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“…5,[33][34][35][36] In discussions of potential mechanisms in the literature it is frequently suggested that nanoclay bioactivity could be attributed to LAPONITE® degradation products. [2][3][4][5][6][7][8]31 Lithium is of particular interest in this context for its well-established pharmacological action as an agonist of the canonical Wnt pathway.…”

Section: Biomaterials Sciencementioning

confidence: 99%

“…[2][3][4][5][6][7][8] For example, it has been suggested that following cellular uptake, clay nanoparticles may undergo degradation within the low pH endosomal or lysosomal intracellular compartments to release dissolution products known to influence osteogenic cell function. [2][3][4][5][6][7][8] In the case of LAPONITE®, these degradation products include orthosilicic acid Si(OH) 4 , magnesium Mg 2+ , and lithium Li + ions. 9,10 Orthosilicic acid Si(OH) 4 is known to promote collagen type 1 synthesis and osteoblast differentiation.…”

Section: Introductionmentioning

confidence: 99%