2008
DOI: 10.1016/j.tsf.2007.04.135
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Nanosecond and femtosecond mass spectroscopic analysis of a molecular beam produced by the spray-jet technique

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(1 citation statement)
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“…Briefly, NR-HS-Ce6-Dox was heated in boiling water for 30 min which resulted in the denaturation of the protein corona, causing the hydrophilic and thermally stable Dox to be released readily into solution. On the other hand, the thermally labile Ce6 was degraded. The solution was then centrifuged at 10,000 rpm for 15 min to remove the NR-HS-Ce6 from the released Dox, and the amount of Dox loaded was quantified by measuring its fluorescence (λ ex /λ em = 480/600 nm) in the supernatant against a set of calibrating standards of known Dox concentrations using Tecan Safire 2 (Tecan Group Ltd., Switzerland).…”
Section: Methodsmentioning
confidence: 99%
“…Briefly, NR-HS-Ce6-Dox was heated in boiling water for 30 min which resulted in the denaturation of the protein corona, causing the hydrophilic and thermally stable Dox to be released readily into solution. On the other hand, the thermally labile Ce6 was degraded. The solution was then centrifuged at 10,000 rpm for 15 min to remove the NR-HS-Ce6 from the released Dox, and the amount of Dox loaded was quantified by measuring its fluorescence (λ ex /λ em = 480/600 nm) in the supernatant against a set of calibrating standards of known Dox concentrations using Tecan Safire 2 (Tecan Group Ltd., Switzerland).…”
Section: Methodsmentioning
confidence: 99%