Nanosecond and femtosecond mass spectroscopic analysis of a molecular beam produced by the spray-jet technique

Yamada, Toshiki; Shinohara, H.; Kamikado, Toshiya; Okuno, Yoshishige; Suzuki, Hitoshi; Mashiko, Shinro; Yokoyama, Shiyoshi

doi:10.1016/j.tsf.2007.04.135

Cited by 1 publication

(1 citation statement)

References 13 publications

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“…Briefly, NR-HS-Ce6-Dox was heated in boiling water for 30 min which resulted in the denaturation of the protein corona, causing the hydrophilic and thermally stable Dox to be released readily into solution. On the other hand, the thermally labile Ce6 was degraded. − The solution was then centrifuged at 10,000 rpm for 15 min to remove the NR-HS-Ce6 from the released Dox, and the amount of Dox loaded was quantified by measuring its fluorescence (λ ex /λ em = 480/600 nm) in the supernatant against a set of calibrating standards of known Dox concentrations using Tecan Safire 2 (Tecan Group Ltd., Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Protein Corona around Gold Nanorods as a Drug Carrier for Multimodal Cancer Therapy

Yeo

Cheah

Lim

et al. 2017

ACS Biomater. Sci. Eng.

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A single nanodevice based on gold nanorods (NRs) coloaded with a photosensitizer, Chlorin e6 (Ce6), and a chemotherapeutic, Doxorubicin (Dox), on its endogenously formed human serum (HS) protein corona, i.e., NR-HS-Ce6-Dox was developed with the aim of performing multimodal cancer therapy: photodynamic (PDT), photothermal (PTT) and chemotherapy (CTX) simultaneously upon irradiation with a single 665 nm laser. Here, the excitation of NRs and Ce6 resulted in photothermal ablation (PTT), and production of reactive oxygen species (ROS) to kill Cal 27 oral squamous cell carcinoma (OSCC) cells by oxidative stress (PDT) respectively, while the laser-triggered release of Dox intercalated into the DNA of cancer cells to result in DNA damage and cell death (CTX). High laser-triggered Dox release efficiency of 71.5% and strong plasmonic enhancement of ROS production by Ce6 (4.8-fold increase compared to free Ce6) was observed. Uptake of both Ce6 and Dox by Cal 27 cells was greatly enhanced, with 3.3 and 52 times higher intracellular Dox and Ce6 fluorescence observed, respectively, 6 h after dosing with NR-HS-Ce6-Dox compared to free drugs. The simultaneous trimodal therapy achieved a near complete eradication of cancer cells (98.7% cell death) with an extremely low dose of 15 pM NR-HS-Ce6-Dox loaded with just 1.26 nM Ce6 and 12.5 nM Dox due to strong synergistic enhancement in cancer cell kill compared to individual therapies performed separately. No dark toxicities were observed. These drug concentrations were far lower than any previously reported in vitro, thus eliminating any potential systemic toxicity of these agents.

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