Nanoscopy on-a-chip: super-resolution imaging on the millimeter scale

Helle, Øystein Ivar; Coucheron, David A.; Tinguely, Jean-Claude; Øie, Cristina Ionica; Ahluwalia, Balpreet Singh

doi:10.1364/oe.27.006700

Cited by 37 publications

(47 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As compared to chip-based SMLM approaches [7,11], chip-based SOFI is an effective technique to generate super-resolved images with relatively higher temporal resolution. Waveguide platform has been previously used TIRF microscopy on living cells [14] and super-resolution imaging of fixed cell using SMLM method [9]. Future work will focus on super-resolution imaging of living cells exploiting chip-based SOFI.…”

Section: Resultsmentioning

confidence: 99%

On-chip TIRF nanoscopy by applying Haar wavelet kernel analysis on intensity fluctuations induced by chip illumination

Jayakumar¹,

Helle²,

Agarwal³

et al. 2020

Opt. Express

Self Cite

View full text Add to dashboard Cite

Photonic-chip based TIRF illumination has been used to demonstrate several on-chip optical nanoscopy methods. The sample is illuminated by the evanescent field generated by the electromagnetic wave modes guided inside the optical waveguide. In addition to the photokinetics of the fluorophores, the waveguide modes can be further exploited for introducing controlled intensity fluctuations for exploitation by techniques such as super-resolution optical fluctuation imaging (SOFI). However, the problem of nonuniform illumination pattern generated by the modes contribute to artifacts in the reconstructed image. To alleviate this problem, we propose to perform Haar wavelet kernel (HAWK) analysis on the original image stack prior to the application of (SOFI). HAWK produces a computational image stack with higher spatio-temporal sparsity than the original stack. In the case of multimoded non-uniform illumination patterns, HAWK processing breaks the mode pattern while introducing spatio-temporal sparsity, thereby differentially affecting the non-uniformity of the illumination. Consequently, this assists nanoscopy methods such as SOFI to better support super-resolution, which is otherwise compromised due to spatial correlation of the mode patterns in the raw image. Furthermore, applying HAWK prior to SOFI alleviates the problem of artifacts due to non-uniform illumination without degrading temporal resolution. Our experimental results demonstrate resolution enhancement as well as reduction in artifacts through the combination of HAWK and SOFI.

show abstract

Section: Resultsmentioning

confidence: 99%

On-chip TIRF nanoscopy by applying Haar wavelet kernel analysis on intensity fluctuations induced by chip illumination

Jayakumar¹,

Helle²,

Agarwal³

et al. 2020

Opt. Express

Self Cite

View full text Add to dashboard Cite

show abstract

“…We have performed resonance Raman measurements of dried hemoglobin at concentration usually found in the red blood cells using the Ta 2 O 5 waveguides. The resonance Raman spectroscopy can also be combined with other complementary optical functions developed using Ta 2 O 5 material, such as optical nanoscopy [26], [27].…”

Section: Discussionmentioning

confidence: 99%

Chip-Based Resonance Raman Spectroscopy Using Tantalum Pentoxide Waveguides

Coucheron

Wadduwage

Murugan

et al. 2019

IEEE Photon. Technol. Lett.

Self Cite

View full text Add to dashboard Cite

Blood analysis is an important diagnostic tool, as it provides a wealth of information about the patient's health. Raman spectroscopy is a promising tool for blood analysis, but widespread clinical application is limited by its low signal strength, as well as complex and costly instrumentation. The growing field of waveguide-based Raman spectroscopy tries to solve these challenges by working toward fully integrated Raman sensors with increased interaction areas. In this letter, we demonstrate resonance Raman measurements of hemoglobin, a crucial component of blood, at 532-nm excitation using a tantalum pentoxide (Ta 2 O 5) waveguide platform. We have also characterized the background signal from Ta 2 O 5 waveguide material when excited at 532 nm. In addition, we demonstrate spontaneous Raman measurements of isopropanol and methanol using the same platform. Our results suggest that Ta 2 O 5 is a promising waveguide platform for resonance Raman spectroscopy at 532 nm and, in particular, for blood analysis.

show abstract

“…The chip-based images presented in this work were performed with rather narrow waveguides of 25 and 70 µm width, where spurious interference patterns are visible. This can be attributed to the limited travel range of the piezo stage holding the coupling objective and can be optimized, as seen in other publications 11,12,15 . More information is provided in the Supplementary Note 5 "Homogeneous illumination with multimode waveguides".…”

Section: Methodsmentioning

confidence: 99%

“…Whereas conventional objective-based TIRF makes use of a single objective for excitation and collection, the chip-based configuration decouples the dependence between the excitation and the collection optics, enabling adjustment-free wavelength multiplexing and free choice of imaging objective without influencing the illumination light path. High intensities (1-10 kW/cm 2 ) in the evanescent field can be achieved by fabricating waveguides made of high-refractive index contrast (HIC) materials (e.g., silicon nitride, Si 3 N 4 ) and by using thin waveguide geometry (150-220 nm thickness) 11 , enabling a chip-based implementation of direct stochastic optical reconstruction microscopy (dSTORM) 11,15 . The use of HIC materials also enables tight confinement of the light inside the photonic-chip.…”

mentioning

confidence: 99%

Photonic-chip assisted correlative light and electron microscopy

et al. 2020

Self Cite

View full text Add to dashboard Cite

Correlative light and electron microscopy (CLEM) unifies the versatility of light microscopy (LM) with the high resolution of electron microscopy (EM), allowing one to zoom into the complex organization of cells. Here, we introduce photonic chip assisted CLEM, enabling multi-modal total internal reflection fluorescence (TIRF) microscopy over large field of view and high precision localization of the target area of interest within EM. The photonic chips are used as a substrate to hold, to illuminate and to provide landmarking of the sample through specially designed grid-like numbering systems. Using this approach, we demonstrate its applicability for tracking the area of interest, imaging the three-dimensional (3D) structural organization of nano-sized morphological features on liver sinusoidal endothelial cells such as fenestrations (trans-cytoplasmic nanopores), and correlating specific endo-lysosomal compartments with its cargo protein upon endocytosis.

show abstract

Nanoscopy on-a-chip: super-resolution imaging on the millimeter scale

Cited by 37 publications

References 28 publications

On-chip TIRF nanoscopy by applying Haar wavelet kernel analysis on intensity fluctuations induced by chip illumination

On-chip TIRF nanoscopy by applying Haar wavelet kernel analysis on intensity fluctuations induced by chip illumination

Chip-Based Resonance Raman Spectroscopy Using Tantalum Pentoxide Waveguides

Photonic-chip assisted correlative light and electron microscopy

Contact Info

Product

Resources

About