Nanoscopic distribution of VAChT and VGLUT3 in striatal cholinergic varicosities suggests colocalization and segregation of the two transporters in synaptic vesicles

Cristofari, Paola; Desplanque, Mazarine; Poirel, Odile; Hébert, Alison; Dumas, Sylvie; Herzog, Étienne; Danglot, Lydia; Geny, David; Gilles, Jean-François; Geeverding, Audrey; Bolte, Susanne; Canette, Alexis; Trichet, Michaël; Fabre, Véronique; Daumas, Stéphanie; Pietrancosta, Nicolas; Mestikawy, Salah El; Bernard, Véronique

doi:10.3389/fnmol.2022.991732

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…Because SODA does not rely on any overlap methods, it is far less sensitive to high-density false positive colocalization artifacts. 1,2 SODA can also be used for all other associations (either direct or distant) in neurosciences 6-10 and even outside this field like, for example, in cell biology, [11][12][13][14][15][16][17][18] virology, 12 in bacteria 19 or plants. 20 Its use has been highlighted in several reviews.…”

Section: Thatmentioning

confidence: 99%

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Molecular mapping of neuronal architecture using STORM microscopy and new fluorescent probes for SMLM imaging

Breton,

Nazac,

Boulet

et al. 2024

Neurophoton.

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Imaging neuronal architecture has been a recurrent challenge over the years, and the localization of synaptic proteins is a frequent challenge in neuroscience. To quantitatively detect and analyze the structure of synapses, we recently developed free SODA software to detect the association of pre and postsynaptic proteins. To fully take advantage of spatial distribution analysis in complex cells, such as neurons, we also selected some new dyes for plasma membrane labeling. Using Icy SODA plugin, we could detect and analyze synaptic association in both conventional and single molecule localization microscopy, giving access to a molecular map at the nanoscale level. To replace those molecular distributions within the neuronal three-dimensional (3D) shape, we used MemBright probes and 3D STORM analysis to decipher the entire 3D shape of various dendritic spine types at the singlemolecule resolution level. We report here the example of synaptic proteins within neuronal mask, but these tools have a broader spectrum of interest since they can be used whatever the proteins or the cellular type. Altogether with SODA plugin, MemBright probes thus provide the perfect toolkit to decipher a nanometric molecular map of proteins within a 3D cellular context.

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Section: Thatmentioning

confidence: 99%

“…SODA can also be used for all other associations (either direct or distant) in neurosciences 6 – 10 and even outside this field like, for example, in cell biology, 11 – 18 virology, 12 in bacteria 19 or plants 20 . Its use has been highlighted in several reviews 20 …”

Section: Colocalization and Coupling Analysis In Conventional And Sup...mentioning

confidence: 99%

Molecular mapping of neuronal architecture using STORM microscopy and new fluorescent probes for SMLM imaging

Breton,

Nazac,

Boulet

et al. 2024

Neurophoton.

Self Cite

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The human VGLUT3-pT8I mutation elicits uneven striatal DA signaling, food or drug maladaptive consumption in male mice

Favier,

Martin Garcia,

Icick

et al. 2024

Nat Commun

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Cholinergic striatal interneurons (ChIs) express the vesicular glutamate transporter 3 (VGLUT3) which allows them to regulate the striatal network with glutamate and acetylcholine (ACh). In addition, VGLUT3-dependent glutamate increases ACh vesicular stores through vesicular synergy. A missense polymorphism, VGLUT3-p.T8I, was identified in patients with substance use disorders (SUDs) and eating disorders (EDs). A mouse line was generated to understand the neurochemical and behavioral impact of the p.T8I variant. In VGLUT3T8I/T8I male mice, glutamate signaling was unchanged but vesicular synergy and ACh release were blunted. Mutant male mice exhibited a reduced DA release in the dorsomedial striatum but not in the dorsolateral striatum, facilitating habit formation and exacerbating maladaptive use of drug or food. Increasing ACh tone with donepezil reversed the self-starvation phenotype observed in VGLUT3T8I/T8I male mice. Our study suggests that unbalanced dopaminergic transmission in the dorsal striatum could be a common mechanism between SUDs and EDs.

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Nanoscopic distribution of VAChT and VGLUT3 in striatal cholinergic varicosities suggests colocalization and segregation of the two transporters in synaptic vesicles

Cited by 2 publications

References 37 publications

Molecular mapping of neuronal architecture using STORM microscopy and new fluorescent probes for SMLM imaging

Molecular mapping of neuronal architecture using STORM microscopy and new fluorescent probes for SMLM imaging

The human VGLUT3-pT8I mutation elicits uneven striatal DA signaling, food or drug maladaptive consumption in male mice

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