Nanoscale Surface Topography Reduces Focal Adhesions and Cell Stiffness by Enhancing Integrin Endocytosis

Li, Xiao; Klausen, Lasse Hyldgaard; Zhang, Wei; Jahed, Zeinab; Tsai, Ching-Ting; Li, Thomas L.; Cui, Bianxiao

doi:10.1021/acs.nanolett.1c01934

Cited by 47 publications

(64 citation statements)

References 54 publications

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“…attachment to the MeHA hydrogel, and SiO2 NPs passivated with RAD remain non-bioactive to influence cell adhesive behaviours. On the other hand, cells cultured on a glass substrate showed a high cell spreading area (Figure S5), consistent with the stem cell behaviour that cells attached well to a rigid substrate [6]. Thus far, these findings suggest that the immobilized SiO2 NPs spatially manipulate RGD localization on their surface to control cell adhesion but the SiO2 NPs without RGD do not intrinsically affect biological functions.…”

Section: Enhanced Mechanosensing Of Hmscs On the Soft Nanocomposite Hydrogelsupporting

confidence: 81%

“…Mesenchymal stem cells (MSCs) are a promising source of regenerative medicine for tissue engineering and regenerative therapeutics. Biophysical cues, such as matrix stiffness [3,4], surface topography [5][6][7], ligand spacing [8,9], and ligand dynamics [10][11][12][13], are shown to regulate cellular behaviors and cell fates. In particular, MSCs can sense and respond to matrix stiffness, which is indicated by Young's modulus and represents the elasticity of the matrix [2].…”

Section: Introductionmentioning

confidence: 99%

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Integrating Soft Hydrogel with Nanostructures Reinforces Stem Cell Adhesion and Differentiation

Yin

Yang

2022

J. Compos. Sci.

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Biophysical cues can regulate stem cell behaviours and have been considered as critical parameters of synthetic biomaterials for tissue engineering. In particular, hydrogels have been utilized as promising biomimetic and biocompatible materials to emulate the microenvironment. Therefore, well-defined mechanical properties of a hydrogel are important to direct desirable phenotypes of cells. Yet, limited research pays attention to engineering soft hydrogel with improved cell adhesive property, which is crucial for stem cell differentiation. Herein, we introduce silica nanoparticles (SiO2 NPs) onto the surface of methacrylated hyaluronic (MeHA) hydrogel to manipulate the presentation of cell adhesive ligands (RGD) clusters, while remaining similar bulk mechanical properties (2.79 ± 0.31 kPa) to that of MeHA hydrogel (3.08 ± 0.68 kPa). RGD peptides are either randomly decorated in the MeHA hydrogel network or on the immobilized SiO2 NPs (forming MeHA–SiO2). Our results showed that human mesenchymal stem cells exhibited a ~1.3-fold increase in the percentage of initial cell attachment, a ~2-fold increase in cell spreading area, and enhanced expressions of early-stage osteogenic markers (RUNX2 and alkaline phosphatase) for cells undergoing osteogenic differentiation with the osteogenic medium on MeHA–SiO2 hydrogel, compared to those cultured on MeHA hydrogel. Importantly, the cells cultivated on MeHA–SiO2 expressed a ~5-fold increase in nuclear localization ratio of the yes-associated protein, which is known to be mechanosensory in stem cells, compared to the cells cultured on MeHA hydrogel, thereby promoting osteogenic differentiation of stem cells. These findings demonstrate the potential use of nanomaterials into a soft polymeric matrix for enhanced cell adhesion and provide valuable guidance for the rational design of biomaterials for implantation.

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Section: Enhanced Mechanosensing Of Hmscs On the Soft Nanocomposite Hydrogelsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Integrating Soft Hydrogel with Nanostructures Reinforces Stem Cell Adhesion and Differentiation

Yin

Yang

2022

J. Compos. Sci.

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“…4 f). In particular, the neurogenesis-related ErbB signaling pathway [ 44 ], cell focal adhesion-related pathways [ 45 , 46 ], and the regulation of actin cytoskeleton [ 47 ] were all found to be enriched ( Fig. 4 g).…”

Section: Resultsmentioning

confidence: 99%

Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiation

Heng

Bai

et al. 2023

Bioactive Materials

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“…[55,56] Employing even thicker NWs (D + ) extends the universality of the study since, for example, also NWs with a diameter of about 1 µm influence cell spreading or facilitate cell transfection. [57,58] Moreover, such larger diameters fill to some extent the gap between NW and micropillar arrays used in cell culture. [59,60] All NW arrays-with NW lengths up to 5 µmwere prepared in a single-step RIE process.…”

Section: Discussionmentioning

confidence: 99%

Generation of Human iPSC‐Derived Neurons on Nanowire Arrays Featuring Varying Lengths, Pitches, and Diameters

Harberts

Siegmund

Hedrich

et al. 2022

Adv Materials Inter

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In this context, vertically aligned high aspect ratio nanostructures-so-called nanowire (NW) arrays-used as cell culture substrates play an increasingly important role in establishing novel tools for interrogating and stimulating cells on molecular and cellular levels. [4][5][6][7] In recent years, studies testing the unique capabilities of NW arrays have been conducted with a variety of cell types, for instance, basic cell lines such as GPE86, HEK293, and HeLa cells, primary rodent neurons, and (mesenchymal) stem cells (MSCs), to name a few. [8,9] The impact of such studies on medical applications such as drug screenings and neurodegenerative disease studies might, however, be significantly improved by employing more sophisticated cells, namely, cells derived from human induced pluripotent stem cells (iPSCs). [10] Human iPSC technologies have changed the way of preclinical research and application by enabling human (patientspecific) in vitro models without restrictions in cell availability. [11] Not only political and ethical controversies raised by using embryonic stem cells (ESCs) are avoided, but also all major cell types including blood-brain barrier models and brain organoids can be generated. [12,13] For studying neurodegenerative diseases such as Alzheimer's or Parkinson's, neurons derived from human iPSCs are of particular interest since adequate models are otherwise scarcely available. [14] Apart from ESCs,

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Nanoscale Surface Topography Reduces Focal Adhesions and Cell Stiffness by Enhancing Integrin Endocytosis

Cited by 47 publications

References 54 publications

Integrating Soft Hydrogel with Nanostructures Reinforces Stem Cell Adhesion and Differentiation

Integrating Soft Hydrogel with Nanostructures Reinforces Stem Cell Adhesion and Differentiation

Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiation

Generation of Human iPSC‐Derived Neurons on Nanowire Arrays Featuring Varying Lengths, Pitches, and Diameters

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