Nanoscale surface modification of thin film microelectrodes to improve biocompatibility

DiPaolo, B.C.; Bagalkot, Naveen; Mohila, C.; Moxon, K. A.

doi:10.1109/iembs.2002.1053171

Cited by 2 publications

(4 citation statements)

References 7 publications

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“…These factors can lead to implant isolation, loss of signal, and ultimately, implant failure. Electrospun fibers have been used to improve the biocompatibility of neural electrodes [1,15,16]. It is therefore possible that the synergistic effects of encapsulating concentration gradients of neurotrophic factors provide a sustained availability of neuroprotective agents and direct neurite penetration towards neural electrodes for enhanced tissue-implant integration and signal transduction.…”

Section: Discussionmentioning

confidence: 99%

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Nanofibrous scaffold with incorporated protein gradient for directing neurite outgrowth

Handarmin

Tan

Sundaray

et al. 2011

Drug Deliv. and Transl. Res.

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Concentration gradient of diffusible bioactive chemicals assumes many important roles in regulating cellular behavior. Among the many factors influencing functional recovery after nerve injury, such as topographical and biochemical signals, concentration gradients of neurotrophic factors provide chemotactic cues for neurite outgrowth and targeted renervation. In this study, a concentration gradient of nerve growth factor (NGF, 0-250 μg/ml) was incorporated throughout the thickness of poly(ε-caprolactone)-poly(ethylene glycol) coaxial electrospun nanofibrous scaffolds (∼700 μm thick with ∼800 nm average fiber diameter). The existence of the protein gradient upon protein release was demonstrated using a customized under-agarose-PC12 neurite outgrowth assay. When exposed to scaffolds endowed with NGF concentration gradient (NGF-CG), a significant difference in the percentage of cells bearing neurite outgrowth was observed (7.1 ± 1.9% vs. 0.8 ± 0.3% for cells exposed to high vs. low concentration surface, respectively; p < 0.05). In contrast, no significant difference was observed when cells were exposed to scaffolds that encapsulated a fixed concentration of NGF. Direct culture of PC12 cells on the substrates demonstrated the cytocompatibility and the effect of diffusible NGF gradient on neurite outgrowth. A significant difference in the percentage of cells with neurite extensions was observed when PC12 cells were seeded on NGF-CG scaffolds (21.2 ± 3.6% vs. 10.4 ± 1.3% on high vs. low concentration surface, respectively; p < 0.05). Furthermore, Z-stack confocal microscopy tracking of neurite extensions revealed the chemotatic guidance effect of NGF concentration gradient. Directed and enhanced neurite penetration into the scaffolds towards increasing NGF concentration was observed. In vitro release study indicated that the encapsulated NGF was released in a sustained manner for at least 30 days (80.4 ± 3.6% released). Taken together, this study demonstrates the feasibility of incorporating concentration gradient of diffusible bioactive chemicals in nanofibrous scaffolds via the coaxial electrospinning technique.

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Section: Discussionmentioning

confidence: 99%

“…Such biomimicking substrates may provide physical cues to direct cell fate [4,9,11,39] and enhance nerve regeneration [8,23,51]. The versatility of the electrospinning process has also allowed the implementation of these nanofibers as surface coatings to enhance neural electrode implant-tissue integration [1,15,16].…”

Section: Introductionmentioning

confidence: 98%

Nanofibrous scaffold with incorporated protein gradient for directing neurite outgrowth

Handarmin

Tan

Sundaray

et al. 2011

Drug Deliv. and Transl. Res.

View full text Add to dashboard Cite

show abstract

“…Electrospun fibers have been used to improve the biocompatibility of neural electrodes. [248][249][250]. It is, therefore, possible that the synergistic effects of encapsulating concentration gradients of neurotrophic factors provide a sustained availability of neuroprotective agents and direct neurite penetration towards neural electrodes for enhanced tissue-implant integration and signal transduction.…”

Section: Direct Pc12 Cell Culture Experimentsmentioning

confidence: 99%

“…Such biomimicking substrates may provide physical cues to direct cell fate [179,180,245,246] and enhance nerve regeneration [106,181,247]. The versatility of the electrospining process has also allowed the implementation of these nanofibers as surface coatings to enhance neural electrode implant-tissue integration [248][249][250].…”

Section: Introductionmentioning

confidence: 99%

Electrospun nanofibers with chemotactic concentration gradient for tissue engineering applications

Handarmin¹

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Nanoscale surface modification of thin film microelectrodes to improve biocompatibility

Cited by 2 publications

References 7 publications

Nanofibrous scaffold with incorporated protein gradient for directing neurite outgrowth

Nanofibrous scaffold with incorporated protein gradient for directing neurite outgrowth

Electrospun nanofibers with chemotactic concentration gradient for tissue engineering applications

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