Nanoscale structure of type I collagen fibrils: Quantitative measurement of D‐spacing

Erickson, Blake; Fang, Ming; Wallace, Joseph M.; Orr, Bradford G.; Les, Clifford M.; Holl, Mark M. Banaszak

doi:10.1002/biot.201200174

Cited by 56 publications

(57 citation statements)

References 47 publications

(84 reference statements)

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“…6,7 Recent AFM studies indicated changes in the nanomorphology of the Type I collagen fibrils in ovariectomized (OVX) sheep [8][9][10] and rats. 11 In order to explore the effect of estrogen depletion and compare efficacy of CatKI and alendronate (ALN) drug treatments, the rabbit OVX model was developed by Merck Inc., as human CatKI's exhibit similar potency for the rabbit enzyme and the adult rabbit skeleton undergoes substantial cortical Haversian remodeling (unlike the rodent).…”

Section: Resultsmentioning

confidence: 99%

“…Details on sample preparation, imaging and image analysis protocols for Type I collagen in bone have been previously published. 6 Images were obtained from locations from across the full 1.00 Â 0.75 cm section of the femur mid-diaphysis (Supplementary Figure S1). All images were acquired in the plane parallel to the long bone axis and obtained from polished regions 100-300 mm below the bone surface.…”

Section: Resultsmentioning

confidence: 99%

“…12 Sample preparation and the AFM imaging methods employed in this work have also been described in detail. 6 All imaging was carried out in air using a PicoPlus 5500 AFM (Agilent, Santa Clara, CA, USA) employing tapping mode with VistaProbes T300R probes (NanoScience, Phoenix, AZ, USA; nominal radius 10 nm, force constant 40 N m À 1 , resonance frequency 300 kHz). Line scan rates were set at 2 Hz or lower at 512 lines per frame.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Alteration of Type I collagen microstructure induced by estrogen depletion can be prevented with drug treatment

Cauble¹,

Rothman²,

Welch³

et al. 2015

BoneKEy Reports

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Two independent biological replicates of estrogen depletion were employed with differing drug treatment conditions. Data Set I consisted of 9-month-old New Zealand white female rabbits treated as follows: sham-operated (n ¼ 11), ovariectomized (OVX; n ¼ 12), OVX þ 200 mg kg À 1 alendronate (ALN), 3 Â a week for 27 weeks (n ¼ 12) and OVX þ 10 mg kg À 1 Cathepsin-K inhibitor (CatKI) daily for 27 weeks. Data Set II consisted of 6-month-old New Zealand white female rabbits that were sham-operated (n ¼ 12), OVX (n ¼ 12) or OVX þ 0.05 mg kg À 1 17b-estradiol (ERT) 3 Â a week for 13 weeks (n ¼ 12). Samples from the cortical femur were polished and demineralized to make them suitable for atomic force microscopy (AFM) imaging. Type I collagen fibrils present in bundles or sheets, running parallel to each other, were combined into a class termed Parallel. Fibrils present outside of such structures, typically in images with an angular range of non-parallel fibrils, were combined into a class termed Oblique. The percentage of fibrils coded as Parallel for Sham animals in Data Sets I and II was 52% and 53%, respectively. The percentage of fibrils coded as Parallel for OVX animals in Data Sets I and II was 35% in both cases. ALN and ERT drug treatments reduced the change from 18 to 12%, whereas CatKI treatment reduced the change to 5%.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Alteration of Type I collagen microstructure induced by estrogen depletion can be prevented with drug treatment

Cauble¹,

Rothman²,

Welch³

et al. 2015

BoneKEy Reports

Self Cite

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“…More specifically, regarding imaging techniques, AFM and electron microscopy techniques can directly image mineralized collagen fibrils, with a FOV covering areas at a scale of tens of nanometres to a few tens of micrometres [9]. Imaging bone at this scale provides useful information on other structural features, such as the collagen -mineral interface [21], mineral platelet sizes and shapes [13,14] or collagen D-period [7,263] for very high-resolution techniques such as TEM and AFM, and offers data revealing structural details of the lacuno-canalicular network [245] and bone remodelling sites [270] for high-resolution techniques such as SEM. X-ray phase-contrast techniques such as ptychographic CT [225] or X-ray phase nanotomography [226] operate at similar scales to volume SEM, and in addition offer the important advantage of tomographic (i.e.…”

Section: Assessment At Different Hierarchical Levels and Additional Imentioning

confidence: 99%

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Georgiadis

Müller

Schneider

2016

J. R. Soc. Interface.

113

100

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Bone's remarkable mechanical properties are a result of its hierarchical structure. The mineralized collagen fibrils, made up of collagen fibrils and crystal platelets, are bone's building blocks at an ultrastructural level. The organization of bone's ultrastructure with respect to the orientation and arrangement of mineralized collagen fibrils has been the matter of numerous studies based on a variety of imaging techniques in the past decades. These techniques either exploit physical principles, such as polarization, diffraction or scattering to examine bone ultrastructure orientation and arrangement, or directly image the fibrils at the sub-micrometre scale. They make use of diverse probes such as visible light, X-rays and electrons at different scales, from centimetres down to nanometres. They allow imaging of bone sections or surfaces in two dimensions or investigating bone tissue truly in three dimensions, in vivo or ex vivo, and sometimes in combination with in situ mechanical experiments. The purpose of this review is to summarize and discuss this broad range of imaging techniques and the different modalities of their use, in order to discuss their advantages and limitations for the assessment of bone ultrastructure organization with respect to the orientation and arrangement of mineralized collagen fibrils.

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“…At each location, 10-15 fibrils were analyzed in error images (B70 fibrils per bone) using two-dimensional fast Fourier transforms (2D FFTs) as described previously. [36][37][38][39] Briefly, 2D FFTs were performed on an area of interest over individual fibrils so that the first harmonic peak from the power spectrum represented the D-spacing for that fibril.…”

Section: Atomic Force Microscopymentioning

confidence: 99%

Exercise prevents β-aminopropionitrile-induced morphological changes to type I collagen in murine bone

Hammond¹,

Wallace²

2015

BoneKEy Reports

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This study evaluated the effects of reduced enzymatic crosslinking, exercise and the ability of exercise to prevent the deleterious impact of reduced crosslinking on collagen D-spacing. Eight-week-old female mice were divided into four weight-matched groups receiving daily injections of either phosphate-buffered saline (PBS) or 300 mg kg À 1 b-aminopropionitrile (BAPN) while undergoing normal cage activity (Sed) or 30 min per day of treadmill exercise (Ex) for 21 consecutive days. BAPN caused a downward shift in the D-spacing distribution in Sed BAPN compared with Sed PBS (Po0.001) but not in Ex BAPN (P ¼ 0.429), indicating that exercise can prevent changes in collagen morphology caused by BAPN. Exercise had no effect on D-spacing in PBS control mice (P ¼ 0.726), which suggests that exercise-induced increases in lysyl oxidase may be a possible mechanism for preventing BAPN-induced changes in D-spacing. The D-spacing changes were accompanied by an increase in mineral crystallinity/maturity due to the main effect of BAPN (P ¼ 0.016). However, no changes in nanoindentation, reference point indentation or other Raman spectroscopy parameters were observed. The ability of exercise to rescue BAPN-driven changes in collagen morphology necessitates further research into the use of mechanical stimulation as a preventative therapy for collagen-based diseases.

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Nanoscale structure of type I collagen fibrils: Quantitative measurement of D‐spacing

Cited by 56 publications

References 47 publications

Alteration of Type I collagen microstructure induced by estrogen depletion can be prevented with drug treatment

Alteration of Type I collagen microstructure induced by estrogen depletion can be prevented with drug treatment

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Exercise prevents β-aminopropionitrile-induced morphological changes to type I collagen in murine bone

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