Nanoscale probes encapsulated by biologically localized embedding (PEBBLEs) for ion sensing and imaging in live cells

Buck, Sarah M.; Xu, Hao; Brasuel, Murphy; Philbert, Martin A.; Kopelman, Raoul

doi:10.1016/j.talanta.2003.12.048

Cited by 151 publications

(103 citation statements)

References 53 publications

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“…58 Their small size and their polymeric nature make them suitable candidates for the generation of micro or nanosensors for intracellular analysis. 75,76 The production of sensor arrays and nanosensors by incorporation of dye molecules into polymeric particles will be discussed later.…”

Section: Fluorescent Polymersmentioning

confidence: 99%

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Design of fluorescent materials for chemical sensing

2007

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There is an enormous demand for chemical sensors for many areas and disciplines. High sensitivity and ease of operation are two main issues for sensor development. Fluorescence techniques can easily fulfill these requirements and therefore fluorescent-based sensors appear as one of the most promising candidates for chemical sensing. However, the development of sensors is not trivial; material science, molecular recognition and device implementation are some of the aspects that play a role in the design of sensors. The development of fluorescent sensing materials is increasingly captivating the attention of the scientists because its implementation as a truly sensory system is straightforward. This critical review shows the use of polymers, sol-gels, mesoporous materials, surfactant aggregates, quantum dots, and glass or gold surfaces, combined with different chemical approaches for the development of fluorescent sensing materials. Representative examples have been selected and they are commented here.

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Section: Fluorescent Polymersmentioning

confidence: 99%

“…These nanoparticles have been used to fabricate H + , O 2 , Ca 2+ , K + , Zn 2+ , Cl 2 , NO 2 2 , O 2 , NO, and glucose sensors. 75 They show very high selectivity, fast response and reversibility. They can be used to obtain information of multiple cells at the same time.…”

Section: Nanosensorsmentioning

confidence: 99%

Design of fluorescent materials for chemical sensing

2007

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“…Another more complex approach for photoswitching has been explored by dye doped nanoparticles allowing coactive triggering of multiple processes. These dye doped sensing nanoparticles were fi rst introduced by Kopelman in the late 1990s and are called photonic explorers for bioanalysis with biologically localized embedding (PEBBLEs) (58) . The insertion of chemosensors into nanoparticles shows several advantages as minimization of interaction with other biomolecules within the cells or the introduction of multifunctional sensing schemes as for example by pH sensitivity.…”

Section: Photoswitchable Fl Uorescent Nanoparticlesmentioning

confidence: 99%

Why not just switch on the light?: light and its versatile applications in the field of nanomedicine

Lehner¹,

Hunziker²

2016

Nano Online

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Over the last decade, the emerging fi eld of nanomedicine has undergone rapid progresses. Different internal and external stimuli like pH, temperature, radiation, ultrasound or light have been introduced to expand the diagnostic and therapeutic options of various applications within the fi eld. This review focuses on the novel application of light in the fi eld of nanomedicine as a mechanism to control drug delivery, release and biochemical and genetic functionality at the target. The fi eld of functional nanomaterials for medicine, and in particular of light responsive nanocarriers, polymers and biomolecules offer new therapeutic options but also requires substantial further research to render this approach broadly applicable in clinical practice.

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“…(8) Notable functional intracellular studies have been undertaken by using "Pebbles" of nano proportions (Photonic Explorers for Bioanalysis with Biologically Localized Embedding). (85) With fine and 20 nano particle chemistry advancing daily, this approach promises a spectrum of sensors covering a wide array of analytes. Perhaps by adjusting the fluorescent characteristics we can acquire multiple boundary layer signatures simultaneously.…”

Section: Non-protein Based Detectionmentioning

confidence: 99%

Windows to cell function and dysfunction: Signatures written in the boundary layers

Smith¹,

Collis²,

Messerli³

2010

BioEssays

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The medium surrounding cells either in culture or in tissues contains a chemical mix varying with cell state. As solutes move in and out of the cytoplasmic compartment they set up characteristic signatures in the cellular boundary layers. These layers are complex physical and chemical environments whose profiles both reflect cell physiology and provide conduits for intercellular messaging. Here we review some of the most relevant characteristics of the extracellular/intercellular space. Our initial focus is primarily with cultured cells but we extend our consideration to the far more complex environment of tissues and discuss how chemical signatures in the boundary layer can or may affect cell function. Critical to the entire essay are the methods used, or being developed, to monitor chemical profiles in the boundary layers. We review recent developments in ultramicro electrochemical sensors and tailored optical reporters suitable for the task 20 in hand. IntroductionThe advent of electron tomography with 3 dimensional image reconstruction has revealed a fundamental beauty and complexity behind the structure of the living cell. Notable contributions have come from several imaging laboratories but one of the most compelling can be found in Marsh et al.(1) From such reconstructions the complexity within a cell is quite staggering and in the 4 th dimension of time this entire structure is in motion. The question that becomes extremely interesting is how molecular and ionic signals are regulated and move within these matrices, where chemical diffusion will be significantly altered. (2) However, complexity, both structural and functional, does not stop at the plasma membrane but extends outwards 30 into the extracellular/intercellular space (EICS: Fig.1). The chemical dynamics within this space are hypothesized to play key roles in cancer, spreading depression, epilepsy and sleep disorders. (3)(4)(5)(6) The purpose of this essay is to consider what we can learn from this space and the methods for following the dynamics of chemical movement within the diffusive boundary layers that comprise an integral part of a cell. We will start by noting the physical and chemical features involved.

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Nanoscale probes encapsulated by biologically localized embedding (PEBBLEs) for ion sensing and imaging in live cells

Cited by 151 publications

References 53 publications

Design of fluorescent materials for chemical sensing

Design of fluorescent materials for chemical sensing

Why not just switch on the light?: light and its versatile applications in the field of nanomedicine

Windows to cell function and dysfunction: Signatures written in the boundary layers

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