Nanoscale Oxidative Patterning of Metallic Surfaces to Modulate Cell Activity and Fate

Vetrone, Fiorenzo; Variola, Fabio; Oliveira, Paulo Tambasco de; Zalzal, S.; Yi, Juyeon; Sam, Johannes; Bombonato-Prado, Karina Fittipaldi; Sarkissian, A.; Perepichka, Dmitrii F.; Wuest, James D.; Rosei, Federico; Nanci, Antonio

doi:10.1021/nl803051f

Cited by 140 publications

(150 citation statements)

References 41 publications

(66 reference statements)

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“…stem cells (MSCs) has largely been provided in vitro. 3,[19][20][21][22][23][24][25][26] Nanostructures support the anabolic path of bone formation, but less information is available with respect to the effect of nanostructures on osteoclasts. The latter is likely of equal importance by virtue of the important role of osteoblastosteoclast coupling and regulation of bone formation and remodeling during osseointegration.…”

Section: Discussionmentioning

confidence: 99%

“…Previous in vitro studies show that nanofeatures stimulate osteoblasts and their progenitors, including the osteogenic differentiation of MSCs. 10,[19][20][21]25,26,[44][45][46] However, many of these studies use osteogenic differentiation media in the culture, and so the effect of nanotopography would be considered as an adjunctive effect. It is therefore interesting that in absence of differentiation media, surface nanotopography did neither induce differentiation nor lineage-specific gene expression changes of MSCs.…”

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confidence: 99%

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The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo

Karazisis¹,

Ballo²,

Petronis³

et al. 2016

IJN

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Purpose Mechanisms governing the cellular interactions with well-defined nanotopography are not well described in vivo. This is partly due to the difficulty in isolating a particular effect of nanotopography from other surface properties. This study employed colloidal lithography for nanofabrication on titanium implants in combination with an in vivo sampling procedure and different analytical techniques. The aim was to elucidate the effect of well-defined nanotopography on the molecular, cellular, and structural events of osseointegration. Materials and methods Titanium implants were nanopatterned (Nano) with semispherical protrusions using colloidal lithography. Implants, with and without nanotopography, were implanted in rat tibia and retrieved after 3, 6, and 28 days. Retrieved implants were evaluated using quantitative polymerase chain reaction, histology, immunohistochemistry, and energy dispersive X-ray spectroscopy (EDS). Results Surface characterization showed that the nanotopography was well defined in terms of shape (semispherical), size (79±6 nm), and distribution (31±2 particles/µm 2 ). EDS showed similar levels of titanium, oxygen, and carbon for test and control implants, confirming similar chemistry. The molecular analysis of the retrieved implants revealed that the expression levels of the inflammatory cytokine, TNF-α , and the osteoclastic marker, CatK , were reduced in cells adherent to the Nano implants. This was consistent with the observation of less CD163-positive macrophages in the tissue surrounding the Nano implant. Furthermore, periostin immunostaining was frequently detected around the Nano implant, indicating higher osteogenic activity. This was supported by the EDS analysis of the retrieved implants showing higher content of calcium and phosphate on the Nano implants. Conclusion The results show that Nano implants elicit less periimplant macrophage infiltration and downregulate the early expression of inflammatory ( TNF -α) and osteoclastic ( CatK ) genes. Immunostaining and elemental analyses show higher osteogenic activity at the Nano implant. It is concluded that an implant with the present range of well-defined nanocues attenuates the inflammatory response while enhancing mineralization during osseointegration.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo

Karazisis¹,

Ballo²,

Petronis³

et al. 2016

IJN

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“…In fact, because the adhesion sites of the cell (focal adhesions) are in the range of 5-200 nm, it is clear that these very small cellular components may be strongly influenced by nanoscale features rather than microscale structures (18). In this frame, nanofabrication techniques may offer interesting tools to achieve a precise control of the surface properties (e.g., controlled nanotexture) and, thus, to evaluate the mechanisms and spatiotemporal aspects of nanomaterial interactions with living systems (19)(20)(21)(22)(23). In this work, we investigated the biological response of human neuroblastoma cells (SH-SY5Y) upon interaction with highly controlled nanostructured metal substrates.…”

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Neurons sense nanoscale roughness with nanometer sensitivity

Brunetti

Maiorano

Rizzello

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

231

213

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The interaction between cells and nanostructured materials is attracting increasing interest, because of the possibility to open up novel concepts for the design of smart nanobiomaterials with active biological functionalities. In this frame we investigated the response of human neuroblastoma cell line (SH-SY5Y) to gold surfaces with different levels of nanoroughness. To achieve a precise control of the nanoroughness with nanometer resolution, we exploited a wet chemistry approach based on spontaneous galvanic displacement reaction. We demonstrated that neurons sense and actively respond to the surface nanotopography, with a surprising sensitivity to variations of few nanometers. We showed that focal adhesion complexes, which allow cellular sensing, are strongly affected by nanostructured surfaces, leading to a marked decrease in cell adhesion. Moreover, cells adherent on nanorough surfaces exhibit loss of neuron polarity, Golgi apparatus fragmentation, nuclear condensation, and actin cytoskeleton that is not functionally organized. Apoptosis/necrosis assays established that nanoscale features induce cell death by necrosis, with a trend directly related to roughness values. Finally, by seeding SH-SY5Y cells onto micropatterned flat and nanorough gold surfaces, we demonstrated the possibility to realize substrates with cytophilic or cytophobic behavior, simply by fine-tuning their surface topography at nanometer scale. Specific and functional adhesion of cells occurred only onto flat gold stripes, with a clear self-alignment of neurons, delivering a simple and elegant approach for the design and development of biomaterials with precise nanostructuretriggered biological responses.nanobiointeractions | nanostructures | patterning T he potential of nanomaterials to trigger specific cellular responses, such as interference and/or activation of defined pathways (1-3), is promising for the development of many important scientific fields, such as regenerative medicine, biotechnology, drug delivery, and nanotoxicity assessment. Initially, cellsmaterials interactions were tackled only from a chemical point of view, because environmental sensing by cells involves specific binding between cellular receptors and ECM ligands. However, recently there has been increasing evidence that the biological response is also affected by the physical properties of the material (4). In particular, it has been demonstrated that cells are influenced by the substrate topography (5, 6), rigidity (7, 8), anisotropy (9, 10), surface charge (11, 12), and wettability (13,14). From this perspective, cellular response to external stimuli goes far beyond the bare ability of the cell to chemically sense specific ECM ligands and includes a wide range of physical cues that are generated at, or act on, the interface between cells and the surrounding environment. For instance, it has been observed that micrometer-scale roughness may affect cell proliferation and morphology (15,16), because it provides a quasi-biomimetic microenvironment to the cells. How...

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“…최근 약학/의학/환경과학분야에서 핵산, 단백질을 이용한 활 성 측정법보다도 세포를 이용한 생리학적반응의 직접 측정 법이 중요시 연구되고있으며, 세포를 다루기 위하여 다양한 마이크로 칩이 개발되고 있다 [1][2][3][4][5][6].…”

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Investigation of Cell Behavior on Nanoporous Surface

Chung¹,

Yoon²,

Min³

2012

KSBB Journal

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1) In this paper, we investigated the effect of nanostructure on the cell behaviors such as adhesion and growth rate. Nanoporous structures with various diameters (30, 40, 45, 50, 60 nm) and 500 nm of the depth were fabricated using the anodizing method. The water contact angle of the surface consisting of nanopores with 30 nm diameter was 40 degree and those were 60~70 degree in cases of nanopores with over 40 nm diameter. Hela cells were cultivated on various nanoporous structure surface to investigate the cell behavior on nanostructure. As a result, Hela cells preferred 30 nm diameter nanoporous surface that has lower water contact angle. This result was confirmed by protein adsorption experiment and scanning electron microscope investigation.

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Nanoscale Oxidative Patterning of Metallic Surfaces to Modulate Cell Activity and Fate

Cited by 140 publications

References 41 publications

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo

Neurons sense nanoscale roughness with nanometer sensitivity

Investigation of Cell Behavior on Nanoporous Surface

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Nanoscale Oxidative Patterning of Metallic Surfaces to Modulate Cell Activity and Fate

Cited by 140 publications

References 41 publications

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and&nbsp;molecular events in vivo

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and&nbsp;molecular events in vivo

Neurons sense nanoscale roughness with nanometer sensitivity

Investigation of Cell Behavior on Nanoporous Surface

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The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo