Nanoscale Glassification of Gold Substrates for Surface Plasmon Resonance Analysis of Protein Toxins with Supported Lipid Membranes

Phillips, K. Scott; Han, Jongho; Martinez, Marilyn N.; Wang, Zhuangzhi; Carter, David; Cheng, Quan

doi:10.1021/ac051644y

Cited by 84 publications

(139 citation statements)

References 56 publications

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“…In order to add high selectivity, biochemical recognition elements, such as antibodies, enzymes, proteins, DNA and cells are immobilized on the solid surface of the SPR sensor [7][8][9][10]. Biochemical recognition elements can be immobilized by physical adsorption [11], by embedding in polymers or membranes [12,13], by trapping in solgels [14,15], and by using functionalized alkanethiol or functionalized alkylsilane self-assembled monolayer (SAM) [16,17]. Each immobilization approach has advantages.…”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Suherman

Morita²,

Kawaguchi

2015

Applied Surface Science

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Surface plasmon resonance sensor equipped with a fabricated immunosensor chip is used for detecting clenbuterol in this study. Since clenbuterol is a small analyte, indirect competitive inhibition immunoassay is employed. For fabricating the immunosurface, the Au-chip was functionalized by succinimidyl-terminated alkanethiol, and the terminal N-hydroxysuccinimide group of the self-assembled monolayer was either replaced with clenbuterol or blocked with ethanolamine. Scanning tunneling microscope experiments and electrochemical measurements depicted the domain structures of the succinimide group of succinimidyl-terminated propanethiol monolayer. The surface concentration and the orientation of succinimide group was significantly dependent on the concentration of dithiobis(succinimidyl) propionate (DSP) used in fabricating the monolayer. Furthermore, the structure of monolayer significantly influenced both the surface concentration and the orientation of clenbuterol on the sensor surface. Consequently, high coverage and standing-up configuration of clenbuterol showed high affinity for clenbuterol antibody. However, high affinity constant exhibited by the sensor surface was coupled with a low sensitivity. By contrast, lowest concentration of DSP solution (0.1 mM) used in fabricating the immunosurface showed a detection sensitivity of 3 ppt-the highest reported sensitivity for clenbuterol. For regeneration the immunosurface, 0.1 M NaOH was used and the same sensor surface could be reused for performing >100 rapid immunoreaction.

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Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Suherman

Morita²,

Kawaguchi

2015

Applied Surface Science

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“…[3][4][5][6] SPR is attractive owing to several inherent advantages (e.g., labelfree analysis, simplicity, cost-effectiveness, and high sensitivity). [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] The SPR resonance angle variation is dependent on various physicochemical processes occurring at the SPR substrate (a thin metal film or a chemically modified metal surface) and/or the substrate/ solution interface. Thus, when coupled with electrochemistry (EC-SPR), it offers a viable avenue to probe optical and electrochemical properties of adsorbates and a sensitive means to quantify thickness variations of ultrathin films accompanying redox reactions.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Surface Plasmon Resonance Spectroscopy at Bilayered Silver/Gold Films

et al. 2006

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Bilayered silver/gold films (gold deposited on top of the silver film) were used as substrates for electrochemical surface plasmon resonance spectroscopy (EC-SPR). EC-SPR responses of electrochemical deposition/stripping of copper and redox-induced conformation changes of cytochrome c immobilized onto self-assembled monolayers preformed at these substrates were measured. Influence of the Ag layer thickness and the double-layer capacitance on the EC-SPR behavior was investigated. The results demonstrated that the bilayered Ag/Au metal films produce a sharper SPR dip profile than pure Au films and retain the high chemical stability of Au films. Contrary to the result by the Fresnel calculation that predicts a greater fraction of Ag in the bilayered film should result in a greater signal-to-noise ratio, the EC-SPR sensitivity is dependent on both the Ag/Au thickness ratio and the chemical modification of the surface. Factors affecting the overall SPR sensitivity at the bilayered films, such as the film morphology, potential-induced excess surface charges, and the adsorbate layer were investigated. Forming a compact adsorbate layer at the bilayered film diminishes the effect of potential-induce excess surface charges on the SPR signal and improves the overall EC-SPR sensitivity. For the case of redox-induced conformation changes of cytochrome c, the SPR signal obtained at the bilayered silver/gold film is 2.7 times as high as that at a pure gold film.

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“…In addition, classic CT determinations require the use of either animal methods (5,6) or tissue culture methods (7,8), which are also time-consuming and are subjective in the interpretation of results. Efforts have been made recently to develop more-sensitive methods, including enzyme-linked immunosorbent assays (9), latex agglutination assays (10), coagglutination assays (5), liposome-based assays (9,11), radioimmunoassays (12), hydrogel-based immunoassays (13), monosaccharide-and antibody array-based assays (14)(15)(16)(17), PCR-based molecular assays (10,(18)(19)(20)(21), and biosensor-based assays (22)(23)(24)(25)(26)(27)(28)(29)(30)(31). A reliable laboratory tool is desirable for clinical services and epidemiological investigation, to characterize CT activities rapidly and quantitatively.…”

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confidence: 99%

Quantitative Detection of Vibrio cholera Toxin by Real-Time and Dynamic Cytotoxicity Monitoring

et al. 2013

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eWe report here the quantitative detection of Vibrio cholerae toxin (CT) in isolates and stool specimens by dynamic monitoring of the full course of CT-mediated cytotoxicity in a real-time cell analysis (RTCA) system. Four cell lines, including Y-1 mouse adrenal tumor cells, Chinese hamster ovary (CHO) cells, small intestine epithelial (FHs74Int) cells, and mouse adrenal gland (PC12-Adh) cells, were evaluated for their suitability for CT-induced cytotoxicity testing. Among them, the Y-1 line was demonstrated to be the most sensitive for CT-mediated cytotoxicity, with limits of detection of 7.0 pg/ml for purified CT and 0.11 ng/ml for spiked CT in pooled negative stool specimens. No CT-mediated cytotoxicity was observed for nontoxigenic V. cholerae, non-V. cholerae species, or non-V. cholerae enterotoxins. The CT-RTCA assay was further validated with 100 stool specimens consecutively collected from patients with diarrhea and 200 V. cholerae isolates recovered from patients and the environment, in comparison to a reference using three detection methods. The CT-RTCA assay had sensitivities and specificities of 97.5% and 100.0%, respectively, for V. cholerae isolates and 90.0% and 97.2% for stool specimens. For stool specimens spiked with CT concentrations ranging from 3.5 pg/ml to 1.8 ng/ml, the inoculation-to-detection time was 1.12 ؎ 0.38 h, and the values were inversely correlated with CT concentrations ( ‫؍‬ ؊1; P ‫؍‬ 0.01). The results indicate that the CT-RTCA assay with the Y-1 cell line provides a rapid and sensitive tool for the quantitative detection of CT activities in clinical specimens. Vibrio cholerae is a Gram-negative, comma-shaped, bacterial pathogen causing cholera, an acute secretory diarrheal disease. Epidemic cholera is common in developing countries and affects about 100,000 people annually (1). Cholera toxin (CT) is a major virulence determinant of V. cholerae, leading to rapidly progressing dehydration, shock, metabolic acidosis, and even death within hours without adequate and appropriate therapy (2). CT is a key biomarker of V. cholerae that is used for forecasting and assessing epidemic disease, monitoring and controlling cholera outbreaks, preventing epidemics, and guiding medical personnel in providing timely treatment of patients. There has been urgent demand for the development of novel sensitive assays for the detection and identification of CT proteins.Conventional biochemical and immunological methods for CT detection and identification can be completed only after V. cholerae is isolated. The bacterial isolation and subsequent CT detection and identification are laborious and usually require several days, resulting in significant delays in patient care and disease control (3, 4). In addition, classic CT determinations require the use of either animal methods (5, 6) or tissue culture methods (7,8), which are also time-consuming and are subjective in the interpretation of results. Efforts have been made recently to develop more-sensitive methods, including enzyme-linked immunosorbent...

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Nanoscale Glassification of Gold Substrates for Surface Plasmon Resonance Analysis of Protein Toxins with Supported Lipid Membranes

Cited by 84 publications

References 56 publications

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Electrochemical Surface Plasmon Resonance Spectroscopy at Bilayered Silver/Gold Films

Quantitative Detection of Vibrio cholera Toxin by Real-Time and Dynamic Cytotoxicity Monitoring

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