Nanoscale Cell Surface Topography Imaging using Scanning Ion Conductance Microscopy

Takahashi, Yasufumi; Ito, Kinuko; Wang, Xiongwei; Matsumae, Yoshiharu; Komaki, Hirokazu; Kumatani, Akichika; Ino, Kosuke; Shiku, Hitoshi; Matsue, Tomokazu

doi:10.5796/electrochemistry.82.331

Cited by 19 publications

(27 citation statements)

References 17 publications

(24 reference statements)

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“…Method 1 assumes that the ratio between the outer and inner diameter of a nanopipette remains constant to that at the nanopipette opening, 13,41,52 and, as such, the inner nanopipette angle can be calculated by using the relationship:…”

Section: Evaluation Of Existing Methods For Nanopipette Characterizationmentioning

confidence: 99%

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Characterization of Nanopipettes

et al. 2016

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Nanopipettes are widely used in electrochemical and analytical techniques as tools for sizing, sequencing, sensing, delivery and imaging. For all of these applications, the response of a nanopipette is strongly affected by its geometry and surface chemistry. As the size of nanopipettes becomes smaller, precise geometric characterization is increasingly important, especially if nanopipette probes are to be used for quantitative studies and analysis. This contribution highlights the combination of data from voltage-scanning ion conductivity experiments, transmission electron microscopy (TEM) and finite element method (FEM) simulations to fully characterize nanopipette geometry and surface charge characteristics, with an accuracy not achievable using existing approaches. Indeed, it is shown that presently used methods for nanopipette characterization can lead to highly erroneous information on nanopipettes. The new approach to characterization further facilitates high-level quantification of the behavior of nanopipettes in electrochemical systems, as demonstrated herein for a scanning ion conductance microscope (SICM) setup.

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Section: Evaluation Of Existing Methods For Nanopipette Characterizationmentioning

confidence: 99%

“…31,[52][53][54]56 Such an approach is very sensitive to the inner half cone angle chosen (equation 3). It has already been seen that the angle can change along the length of the nanopipette (Figure 1c) and will vary depending on the pulling parameters used for nanopipette fabrication.…”

Section: Characterization Of Nanopipettes In High Ionic Strength Mediamentioning

confidence: 99%

Characterization of Nanopipettes

et al. 2016

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“…Living cells, which are demanding samples owing to the need of a warm, liquid environment, have successfully been studied with both AFM 6-10 and SICM. 4,[11][12][13][14] Both techniques have also been combined. 9,15,16 The imaging mechanism in AFM is based on mechanical forces between the sample and the tip of a cantilever, which is used to track the surface of the sample (Fig.…”

Section: Introductionmentioning

confidence: 98%

Comparison of Atomic Force Microscopy and Scanning Ion Conductance Microscopy for Live Cell Imaging

et al. 2015

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Atomic force microscopy (AFM) and scanning ion conductance microscopy (SICM) are excellent and commonly used techniques for imaging the topography of living cells with high resolution. We present a direct comparison of AFM and SICM for imaging microvilli, which are small features on the surface of living cells, and for imaging the shape of whole cells. The imaging quality on microvilli increased significantly after cell fixation for AFM, whereas for SICM it remained constant. The apparent shape of whole cells in the case of AFM depended on the imaging force, which deformed the cell. In the case of SICM, cell deformations were avoided, owing to the contact-free imaging mechanism. We estimated that the lateral resolution on living cells is limited by the cell's elastic modulus for AFM, while it is not for SICM. By long-term, time-lapse imaging of microvilli dynamics, we showed that the imaging quality decreased with time for AFM, while it remained constant for SICM.

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“…SICM Setup: The SICM setup has been described previously. [41] Briefly, the system consisted of an XY Piezo Stage (621.2CL, Physik Instrumente, Germany) that moved the sample within a 200 × 200 μm 2 travel range and a Z-Stage (621.ZCL, Physik Instrumente) with a 25 μm travel range. All piezo stages were controlled using a controller module (E-501, Physik Instrumente).…”

Section: Methodsmentioning

confidence: 99%

Topography and Permeability Analyses of Vasculature‐on‐a‐Chip Using Scanning Probe Microscopies

Nashimoto

Abe

Fujii

et al. 2021

Adv Healthcare Materials

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Microphysiological systems (MPS) or organs-on-chips (OoC) can emulate the physiological functions of organs in vitro and are effective tools for determining human drug responses in preclinical studies. However, the analysis of MPS has relied heavily on optical tools, resulting in difficulties in real-time and high spatial resolution imaging of the target cell functions. In this study, the role of scanning probe microscopy (SPM) as an analytical tool for MPS is evaluated. An access hole is made in a typical MPS system with stacked microchannels to insert SPM probes into the system. For the first study, a simple vascular model composed of only endothelial cells is prepared for SPM analysis. Changes in permeability and local chemical flux are quantitatively evaluated during the construction of the vascular system. The morphological changes in the endothelial cells after flow stimulation are imaged at the single-cell level for topographical analysis. Finally, the possibility of adapting the permeability and topographical analysis using SPM for the intestinal vascular system is further evaluated. It is believed that this study will pave the way for an in situ permeability assay and structural analysis of MPS using SPM.

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Nanoscale Cell Surface Topography Imaging using Scanning Ion Conductance Microscopy

Cited by 19 publications

References 17 publications

Characterization of Nanopipettes

Characterization of Nanopipettes

Comparison of Atomic Force Microscopy and Scanning Ion Conductance Microscopy for Live Cell Imaging

Topography and Permeability Analyses of Vasculature‐on‐a‐Chip Using Scanning Probe Microscopies

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