Nanoreactors based on (polymerized) ABA-triblock copolymer vesicles

Nardin, Corinne; Thoeni, Sandra; Widmer, J.; Winterhalter, Mathias; Meier, Wolfgang

doi:10.1039/b004280n

Cited by 307 publications

(320 citation statements)

References 11 publications

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“…Larger pores, specifically their protein membrane channels as taken from biological membranes, were reconstituted by Meier and coworkers for the first time in the polymeric PMOXA-PDMS-PMOXA membrane [32]. The PMOXA-PDMS-PMOXA membrane is impermeable to small molecules, including water [36], and its permeability was increased by reconstituting channel proteins, allowing the transport of various molecules through the polymeric membrane (Figure 7).…”

Section: Reconstituting Membrane Proteins In Polymeric Membranesmentioning

confidence: 99%

“…When reconstituting membrane proteins in polymeric membranes, one must consider that mild preparation conditions must be used in order to preserve their activities [23,32]. Another consideration, when working with membrane proteins and polymeric membranes, is the extent of reconstitution, a determination of which has not been done to date.…”

Section: Ampicillinoic Acid Ompfmentioning

confidence: 99%

“…Meier and coworkers developed various methods to prepare nanovesicles of triblock copolymers poly(2-methyl-2-oxazoline)-blockpoly(dimethyl siloxane)-block-poly(2-methyl-2-oxazoline) (PMOXA-PDMS-PMOXA), and were able to reconstitute membrane proteins [32] such as OmpF (outer membrane protein F) [34] and FhuA [35]. Another approach is to consider enzymes as hydrophilic blocks and, together with a hydrophobic polymer domain, these generate a bilayer [39].…”

Section: Polymeric Membranesmentioning

confidence: 99%

“…On the other hand, reconstitution of membrane proteins in polymer membranes is far from being understood. Membrane proteins insert into polymeric membranes and they maintain their functions in spite of their thicknesses (~10 nm, [32]). Theoretical models explain this in the following way: The insertion of proteins is possible due to the compressibility of polymeric membranes, which is not the case for lipid membranes, as shown in Figure 8 [62].…”

Section: Reconstituting Membrane Proteins In Polymeric Membranesmentioning

confidence: 99%

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Bio-Decorated Polymer Membranes: A New Approach in Diagnostics and Therapeutics

et al. 2011

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Today, demand exists for new systems that can meet the challenges of identifying biological entities rapidly and specifically in diagnostics, developing stable and multifunctional membranes, and engineering devices at the nanometer scale. In this respect, bio-decorated membranes combine the specificity and efficacy of biological entities, such as peptides, proteins, and DNA, with stability and the opportunity to chemically tailor the properties of polymeric membranes. A smart strategy that serves to fulfill biological criteria is required, whereby polymer membranes come to mimic biological membranes and do not disturb but rather enhance the functioning and activity of a biological entity. Different approaches have been developed, exemplified by either planar or vesicular membranes, allowing insertion inside the polymer membrane or anchoring via functionalization of the membrane surface. Inspired by nature, but incorporating the strength provided by chemical design, bio-decorated polymer membranes represent a novel concept with great potential in diagnostics and therapeutics.

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Section: Reconstituting Membrane Proteins In Polymeric Membranesmentioning

confidence: 99%

Section: Ampicillinoic Acid Ompfmentioning

confidence: 99%

Section: Polymeric Membranesmentioning

confidence: 99%

Section: Reconstituting Membrane Proteins In Polymeric Membranesmentioning

confidence: 99%

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Bio-Decorated Polymer Membranes: A New Approach in Diagnostics and Therapeutics

et al. 2011

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“…By combining encapsulation strategies with membranes decorated with transmembrane protein channels, block copolymer vesicles have been used as nanoreactors. 7,11 These function by allowing substrates into the vesicle under reversible voltage gating of the channels, where they are acted upon by an encapsulated enzyme. Another promising direction for controlled release is to use a degradable hydrophobic block which causes destabilization of the assembly with time.…”

Section: Introductionmentioning

confidence: 99%

Computer simulation of aqueous block copolymer assemblies: Length scales and methods

Ortiz

Nielsen

Klein

et al. 2006

J Polym Sci B Polym Phys

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Atomistic, coarsegrain, and mesoscopic computer simulation methods applied recently to the study of block copolymer assemblies in solution are reviewed. At the atomic level, particle-based simulations have provided insight into specific interactions such as hydrogen bonding between polymer and water.Coarse-grain models have given a generalized view of the conformations of polymer chains in solvents of different qualities by grouping clusters of atoms into effective interaction sites. Mesoscopic selfconsistent field theory allows for the study of the phase diagram of block copolymers in water using a mean-field continuum description. [1907][1908][1909][1910][1911][1912][1913][1914][1915][1916][1917][1918] 2006 Keywords: all-atom; coarse-grain; DPD; field theory; molecular dynamics Kingston, ON in 1996. In 1998, he received his M.Sc. and Ph.D. degrees from the University of Toronto for his work on the statistical mechanics of mixed quantum-classical systems under the supervision of Raymond E. Kapral. He then moved on to join the group of Michael L. Klein at the University of Pennsylvania as a postdoctoral research associate to work on coarse-grain models of membranes. His current research interests are on the solubilization of carbon nanotubes by cyclic peptides and on a detailed molecular understanding of colloidal stability.

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Polymer Vesicles

Förster¹,

Borchert²

2005

Encyclopedia of Polymer Science and Technology

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Vesicles are self‐supported closed bilayer assemblies of amphiphiles that enclose an aqueous interior volume. The most important vesicle‐forming amphiphiles are phospholipids forming liposomes , as well as amphiphilic block copolymers forming polymersomes . Vesicles are investigated with respect to biomedical and cosmetic applications. The article covers energy and kinetic aspects of bilayer and vesicle formation, vesicle preparation methods, classification of vesicle shapes and shape transitions, mechanical properties of vesicle membranes, methods to improve vesicle stability, and biomedical aspects such as loading and sustained or triggered release of encapsulants, reconstitution of membrane proteins, and cell adhesion. An overview of all vesicle‐forming polymers is given. Theoretical concepts such as curvature and elasticity energy are introduced and their consequence for vesicle formation, size, and shape are discussed.

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Nanoreactors based on (polymerized) ABA-triblock copolymer vesicles

Abstract: A new kind of nanoreactor has been prepared by the incorporation of a channel protein into the shell of (polymerized) vesicles formed from an amphiphilic ABA-triblock copolymer.

Cited by 307 publications

References 11 publications

Bio-Decorated Polymer Membranes: A New Approach in Diagnostics and Therapeutics

Bio-Decorated Polymer Membranes: A New Approach in Diagnostics and Therapeutics

Computer simulation of aqueous block copolymer assemblies: Length scales and methods

Polymer Vesicles

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