Nanoprobes for intracellular and single cell surface‐enhanced Raman spectroscopy (SERS)

Vitol, Elina A.; Orynbayeva, Zulfiya; Friedman, Gary D.; Gogotsi, Yury

doi:10.1002/jrs.3100

Cited by 70 publications

(56 citation statements)

References 128 publications

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“…The geometrical configuration of a metal nanostructure in addition to its electromagnetic properties defines the enhancement activity. 47, 48 In the homo-peptide experiments, the proportion of spectra with suppressed amide I bands varies with the excitation wavelength/metal identity. Spectra with suppressed bands are observed more frequently in SERS with silver island films (530 nm excitation) than with gold nanoparticles (785 nm excitation) (Table 2).…”

Section: Resultsmentioning

confidence: 99%

Amide I vibrational mode suppression in surface (SERS) and tip (TERS) enhanced Raman spectra of protein specimens

Kurouski

Postiglione

Deckert‐Gaudig

et al. 2013

Analyst

151

154

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Surface- and tip-enhanced Raman spectroscopy (SERS and TERS) are modern spectroscopic techniques, which are becoming widely used and show a great potential for the structural characterisation of biological systems. Strong enhancement of the Raman signal through localised surface plasmon resonance enables chemical detection at the single-molecule scale. Enhanced Raman spectra collected from biological specimens, such as peptides, proteins or microorganisms, were often observed to lack the amide I band, which is commonly used as a marker for the interpretation of secondary protein structure. The cause of this phenomenon was unclear for many decades. In this work, we investigated this phenomenon for native insulin and insulin fibrils using both TERS and SERS and compared these spectra to the spectra of well-defined homo peptides. The results indicate that the appearance of the amide I Raman band does not correlate with the protein aggregation state, but is instead determined by the size of the amino acid side chain. For short model peptides, the absence of the amide I band in TERS and SERS spectra correlates with the presence of a bulky side chain. Homo-glycine and -alanine, which are peptides with small side chain groups (H and CH3, respectively), exhibited an intense amide I band in almost 100% of the acquired spectra. Peptides with bulky side chains, such as tyrosine and tryptophan, exhibited the amide I band in 70% and 31% of the acquired spectra, respectively.

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Section: Resultsmentioning

confidence: 99%

Amide I vibrational mode suppression in surface (SERS) and tip (TERS) enhanced Raman spectra of protein specimens

Kurouski

Postiglione

Deckert‐Gaudig

et al. 2013

Analyst

151

154

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“…A great selection of reviews exist which go into further detail than is presented here for other related areas within SERS. These reviews include general overviews [267][268][269][270][271], and reviews specific to cell studies [272][273][274][275], medical applications [24,25,276,277], and toxicology [278,279].…”

Section: Surface-enhanced Raman Scattering (Sers)mentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

254

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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“…Surface-enhanced Raman spectroscopy (SERS), allows the detection limits of conventional Raman scattering to be overcome by utilizing the effect of electric field enhancement in the vicinity of metal nanoparticles. Colloidal metal nanoparticles are traditionally used for such measurements; however, these particles have a tendency to agglomerate and move within the cell, which alters the amplification effect, hindering the applications of this method for long-term experiments and for obtaining reproducible data [30]. In view of this, SERS-active nanoprobes with controlled and fixed configuration of plasmonic nanoparticles are required for reliable intracellular SERS sensing.…”

Section: Capabilities Performance and Applications Of Pointed Probesmentioning

confidence: 98%

One-Dimensional Nanoprobes for Single-Cell Studies

et al. 2013

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Owing to variation of individual cells within a population, single-cell studies are of great interest to researchers. Recent developments in nanofabrication technology have made this area increasingly attractive as one-dimensional (1D) nanoscale probes can be manufactured with increasing accuracy. Here, we provide an overview and description of the major designs that have been reported to date. For more details of what applications could be realized and how, based on the probe shapes and designs, we summarize the most recently reported performances of 1D single-cell probes with their advantages and limitations. Minimally invasive probes are required for long-term experiments on single cells. Carbon nanotubes with their unique properties and structure are excellent candidates for multitask robotic intracellular probes. Carbon nanotube-tipped cellular endoscopes are less invasive compared with pipettes or cantilever tips. Advances in nanofabrication techniques have made it possible to produce more consistent nanoscale cellular probes that can capture a variety of information from optical, electrical and chemical signals. In addition, these tools can transfer tiny amounts of fluids and molecular materials in a highly localized fashion for the purpose of analyzing or stimulating a variety of responses at the level of individual cells and even cellular organelles. We conclude with a critical analysis of the current state of the field as well as the major obstacles for further probe development of minimally invasive probes and their widespread use in cell biology.

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Nanoprobes for intracellular and single cell surface‐enhanced Raman spectroscopy (SERS)

Cited by 70 publications

References 128 publications

Amide I vibrational mode suppression in surface (SERS) and tip (TERS) enhanced Raman spectra of protein specimens

Amide I vibrational mode suppression in surface (SERS) and tip (TERS) enhanced Raman spectra of protein specimens

Raman spectroscopy: techniques and applications in the life sciences

One-Dimensional Nanoprobes for Single-Cell Studies

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