Nanopriming Action of Microwave-Assisted Biofunctionalized ZnO Nanoparticles to Enhance the Growth under Moisture Stress in Vigna radiata

Abstract

Bare and stabilized zinc oxide nanoparticles (ZnO NPs) were prepared by a microwave-assisted method and used as a priming agent to improve the morphological, physiological, and biochemical quality of Vigna radiata. The priming action was made under normal and moisture stress conditions. A microwave reactor of 850 watts power was used to heat 30 mL of a nanocolloidal solution at 140 °C for 20 min. The stable spherical ZnO NPs at 50.4 mV with 28.2 nm particle size were generated and capped with different biomolecules, cysteine and PVA, to get biostabilized ZnO NPs at 48.8 and 108.5 nm with ζ potentials of −56.2 and −52.0 mV, respectively, holding distinct morphology. The nanopriming effect was studied in V. radiata seeds for bare ZnO and capped ZnO NPs under normal and moisture stress environments. Cysteine-capped ZnO NPs at 250 ppm showed improved germination (90 and 76%), radicle growth (7.6 and 3.6 cm), seedling Vigor (3064 and 1816), dry matter production (145.06 and 96.92 mg/25 seedlings), and hydrolytic (α-amylase and protease) and antioxidant (peroxidase and superoxide dismutase) enzyme activity under normal and moisture stress conditions. The improved priming action of cysteine-capped ZnO NPs is due to increased cell elongation and cell division in the radicle. The uptake and translocation of ZnO NPs in the V. radiata root are evidenced by the presence of an 11.4 ppm zinc level, which was also supported by EDAX and FITC labeling results.

Nanopriming and AI for Sustainable Agriculture: Boosting Seed Germination and Seedling Growth with Engineered Nanomaterials, and Smart Monitoring through Deep Learning

“…Recently, various metal-based ENMs, including nanoparticles (NPs) like ZnO NPs, Fe NPs, Ag NPs, and Ag/ZnO NPs have demonstrated potential in enhancing seed germination rates (SGR) even under stressful conditions. ZnO NPs, in particular, have emerged as highly applicable ENMs in agriculture. , Zinc, a vital micronutrient, plays a crucial role in supporting optimal plant growth by facilitating processes, such as synthesis of photosynthetic pigments, cell division, and overall plant development . ZnO NPs have been found to positively impact shoot growth rate, plant growth, and yield, even under stress conditions .…”