Nanopore Targeted Sequencing for the Accurate and Comprehensive Detection of SARS‐CoV‐2 and Other Respiratory Viruses

Non-enzymatic signal amplification-powered point-of-care SERS sensor for rapid and ultra-sensitive assay of SARS-CoV-2 RNA

“…For high sensitive and specific detection of SARS-CoV-2 RNA, the wildly-agreed gene fragment in the open reading frame 1 ab (ORF1ab) region with highly conserved viral nucleic acid sequences was selected as the target sequence ( Li et al, 2021 ; Udugama et al, 2020 ; Wang et al, 2021 ). Scheme 1 b illustrates the unlocking-mediated target recycling signal amplification strategy for SERS detection of SARS-CoV-2 RNA, which includes five parts, i.e., LPs, H2, SERS tags, SERS sensing chip and analyte.…”

Rapid genotyping of tilapia lake virus (TiLV) using Nanopore sequencing

“…Such application of Nanopore has been successfully applied to study human epidemics such as Ebola virus in remote areas of West Africa (Hoenen et al., 2016), the Zika virus in hard to reach regions of Brazil (Faria et al., 2016). More recently, the technology was used to sequence and identify SARS‐CoV‐2, the virus causing the COVID‐19 pandemic (Wang et al., 2020).…”

NanoRTax, a real-time pipeline for taxonomic and diversity analysis of nanopore 16S rRNA amplicon sequencing data

“…While NanoRTax was designed for full-length 16S rRNA gene taxonomic analysis of microbial samples, a focus on different ampli cation targets and the use of pipeline parametrization could take the application beyond bacterial pro ling. Similar NanoRTax-based classi cation work ows can be proposed for the detection of fungal and viral infections [19,20], while non-taxonomic targeted amplicons can pro le either speci c antimicrobial resistance genes or entire gene panels such as the resistome [21]. Furthermore, continuous releases of the ONT sequencing chemistry and improvements in the basecalling algorithms are expected to positively impact taxonomic assignments using NanoRTax.…”