Nanopore Sensing Technique for Studying the Hofmeister Effect

Abstract

The nanopore sensing technique is an emerging method of detecting single molecules, and extensive research has gone into various fields, including nanopore sequencing and other applications of single‐molecule studies. Recently, several researchers have explored the specific ion effects in nanopore channels, enabling a unique understanding of the Hofmeister effect at the single‐molecule level. Herein, the recent advances of using nanopore sensing techniques are reviewed to study the Hofmeister effect and the physicochemical mechanism of this process is attempted. The challenges and goals are also discussed for the future in this field.

Correlation coefficient-directed label-free characterization of native proteins by surface-enhanced Raman spectroscopy

“…When ions are added to the solution, they will exhibit different salting abilities, whose typical order is known as the Hofmeister series (cations, experimental methods, such as infrared spectroscopy, dynamic light scattering, NMR and nanopore are useful tools for studying the Hofmeister effects on protein behavior at high concentrations. [40][41][42][43][44] It is of great signicance to develop a quantitative method to describe and interpret the Hofmeister effect on the protein structure at low concentrations close to the physiological concentration (<0.1 M), which has rarely been reported. To this end, we sought to investigate the ion effect on the protein at low concentrations (0.01 M and 0.1 M) by D-AuIMNPs.…”

Hofmeister Effects Shine in Nanoscience

“…Dynamic light scattering (DLS), nuclear magnetic resonance (NMR) spectroscopy and dark field microscopy, atomic force microscopy (AFM), and nanopores DOI: 10.1002/advs.202302057 are powerful experimental tools for studying the Hofmeister effects. [9,[15][16][17][18][19] DLS can measure very quantitative information such as hydrodynamic diameters. NMR spectroscopy can study the binding affinity of ions in various systems.…”

Ion‐specific effects in confined nanochannels and neural network

“…Thus, the most important application of nanopores is that they are used as building blocks for artificial neurons by regulating ion‐specific effects. [ 5–8 ]…”

“…Thus, the most important application of nanopores is that they are used as building blocks for artificial neurons by regulating ion-specific effects. [5][6][7][8] Ions diffuse in a very "aggregated" style inside the nanochannels, which are the basis of the matter, information, and energy exchange in the biological system especially for nervous system. [9,10] Using nanopores as a biomimetic model for simulating the ion channels in the neurons will assist in investigating the ion-specific effect in confined space from a neurophysiological perspective.…”