Nanopore Sensing

Shi, Wenqing; Friedman, Alicia K.; Baker, Lane A.

doi:10.1021/acs.analchem.6b04260

Cited by 359 publications

(331 citation statements)

References 250 publications

Supporting

Mentioning

331

Contrasting

Order By: Relevance

“…The recent rapid development of solid‐state nanopores represents a robust and durable sensing platform to perform single biomolecule detection, DNA sequencing, and proteomic profiling . Synthetic nanopores not only approach the small size of biological ion channels, but can also be designed to incorporate voltage‐gating properties and biorecognition capabilities to mediate analyte transport into the nanopore, where chemical signals can be generated . Some biomimetic nanopores behave like ionic diodes, allowing ions or molecules to flow in one direction while suppressing motion in the reverse direction .…”

Section: Introductionmentioning

confidence: 99%

Voltage‐Gated Nanoparticle Transport and Collisions in Attoliter‐Volume Nanopore Electrode Arrays

Han

Crouch

et al. 2018

Small

View full text Add to dashboard Cite

Single nanoparticle analysis can reveal how particle‐to‐particle heterogeneity affects ensemble properties derived from traditional bulk measurements. High‐bandwidth, low noise electrochemical measurements are needed to examine the fast heterogeneous electron‐transfer behavior of single nanoparticles with sufficient fidelity to resolve the behavior of individual nanoparticles. Herein, nanopore electrode arrays (NEAs) are fabricated in which each pore supports two vertically spaced, individually addressable electrodes. The top ring electrode serves as a particle gate to control the transport of silver nanoparticles (AgNPs) within individual attoliter volume NEAs nanopores, as shown by redox collisions of AgNPs collisions at the bottom disk electrode. The AgNP‐nanoporeis system has wide‐ranging technological applications as well as fundamental interest, since the transport of AgNPs within the NEA mimics the transport of ions through cell membranes via voltage‐gated ion channels. A voltage threshold is observed above which AgNPs are able to access the bottom electrode of the NEAs, i.e., a minimum potential at the gate electrode is required to switch between few and many observed collision events on the collector electrode. It is further shown that this threshold voltage is strongly dependent on the applied voltage at both electrodes as well as the size of AgNPs, as shown both experimentally and through finite‐element modeling. Overall, this study provides a precise method of monitoring nanoparticle transport and in situ redox reactions within nanoconfined spaces at the single particle level.

show abstract

Section: Introductionmentioning

confidence: 99%

Voltage‐Gated Nanoparticle Transport and Collisions in Attoliter‐Volume Nanopore Electrode Arrays

Han

Crouch

et al. 2018

Small

View full text Add to dashboard Cite

show abstract

“…[1][2][3] However, multiparameter analysis of NPs at the single NP level in ionic solution remains challenging and efficient and high-precision methods are still limited and at the early developing stage. [6][7][8] Utilizing the ionic current change induced by the NP translocation event, information of target analyte, including number density, size, shape, charge and even the dynamic orientation and motion speed during translocation can be obtained. [6][7][8] Utilizing the ionic current change induced by the NP translocation event, information of target analyte, including number density, size, shape, charge and even the dynamic orientation and motion speed during translocation can be obtained.…”

Section: Introductionmentioning

confidence: 99%

Probing Dynamic Events of Dielectric Nanoparticles by a Nanoelectrode‐Nanopore Nanopipette

et al. 2018

View full text Add to dashboard Cite

In recent years, various single-entity electrochemical methods have been developed to study the dynamic events of single nanoparticles (NPs) in solution. It has been demonstrated that the assembly, transport, and translocation of metallic NPs near a nanopore entrance can be detected based on the open-circuit potential change of a floating nanoelectrode placed near the nanopore. In this study, we applied the nanopore-nanoelectrode-based method to study polystyrene (PS) NPs with various sizes, which were used as the model dielectric NPs. Furthermore, by utilizing dielectrophoretic (DEP) force applied through the nanoelectrode, PS NPs can be efficiently preconcentrated to form large assemblies outside the nanopipette tip, enabling high-throughput single NP analysis. We revealed how the interactions between NPs and between the NP and the nanopore surface affected the current and potential signals. We investigated the dynamic structures and motions of PS NPs inside the large assembly based on the complementary and correlated the ionic current and potential signals from both the nanopore and the nanoelectrode. We also compared the difference in the dynamic events between polarizable metallic NPs and non-polarizable dielectric NPs during multi-NP structure formation and individual NP transport and translocation motions.

show abstract

“…In the very first SICM study [1], for example, areas of high conductance were used to identify pores in a membrane. Further work has considered the imaging of both synthetic [69,70] and biological [71,72] nanopores, with quantitative measurements of individual nanopores possible [73]. The SICM nanopipette can also be used as a reservoir of analyte (e.g.…”

Section: (D) Conductance Measurements Local Delivery and Sizingmentioning

confidence: 99%

Multifunctional scanning ion conductance microscopy

2017

View full text Add to dashboard Cite

Scanning ion conductance microscopy (SICM) is a nanopipette-based technique that has traditionally been used to image topography or to deliver species to an interface, particularly in a biological setting. This article highlights the recent blossoming of SICM into a technique with a much greater diversity of applications and capability that can be used either standalone, with advanced control (potential-time) functions, or in tandem with other methods. SICM can be used to elucidate functional information about interfaces, such as surface charge density or electrochemical activity (ion fluxes). Using a multi-barrel probe format, SICM-related techniques can be employed to deposit nanoscale three-dimensional structures and further functionality is realized when SICM is combined with scanning electrochemical microscopy (SECM), with simultaneous measurements from a single probe opening up considerable prospects for multifunctional imaging. SICM studies are greatly enhanced by finite-element method modelling for quantitative treatment of issues such as resolution, surface charge and (tip) geometry effects. SICM is particularly applicable to the study of living systems, notably single cells, although applications extend to materials characterization and to new methods of printing and nanofabrication. A more thorough understanding of the electrochemical principles and properties of SICM provides a foundation for significant applications of SICM in electrochemistry and interfacial science.

show abstract

Nanopore Sensing

Cited by 359 publications

References 250 publications

Voltage‐Gated Nanoparticle Transport and Collisions in Attoliter‐Volume Nanopore Electrode Arrays

Voltage‐Gated Nanoparticle Transport and Collisions in Attoliter‐Volume Nanopore Electrode Arrays

Probing Dynamic Events of Dielectric Nanoparticles by a Nanoelectrode‐Nanopore Nanopipette

Multifunctional scanning ion conductance microscopy

Contact Info

Product

Resources

About