“…As analytes pass through the pore, driven by electrophoretic force, ions are depleted, causing ionic current blockage associated with the analyte's volume, conformation, or size 22 . The technique enables single-molecule detection and has facilitated the study of a wide range of biological species, including DNA 23,24 , proteins 19,25,26 antibodies 19,27 , RNAs [28][29][30][31] , ribosomes 32 , and viruses 33,34 . Recently, DNA-based labelling of RNA has been performed, allowing for RNA structural mapping with nanopores 35 .…”