Nanopartikel und Nanoaerosole — Messmethoden

Szymanski, Wladyslaw W.; Allmaier, Günter

doi:10.1007/978-3-211-49303-8_2

Cited by 1 publication

(2 citation statements)

References 23 publications

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“…The DMA was set to transfer particles with an EMD of 8.6, 27.9, 33.0, 47.6, and 61.0 nm (±5 nm approximation) to the ENPS. 43 The particles of an EMD of 10 nm or less were assumed to be buffer residues and the detergent. Particles with an EMD of 8.6 nm did not show any color reaction on the membrane, due to the lack of antibody recognition in this fraction, and no particle was visualized by AFM.…”

Section: Resultsmentioning

confidence: 99%

“…For a detailed investigation of the recorded GEMMA spectrum (Figure ) of the TBEV-sample, separated peaks were selected with the DMA to collect these particles of the chosen EMD. The DMA was set to transfer particles with an EMD of 8.6, 27.9, 33.0, 47.6, and 61.0 nm (±5 nm approximation) to the ENPS . The particles of an EMD of 10 nm or less were assumed to be buffer residues and the detergent.…”

Section: Resultsmentioning

confidence: 99%

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Comprehensive Size-Determination of Whole Virus Vaccine Particles Using Gas-Phase Electrophoretic Mobility Macromolecular Analyzer, Atomic Force Microscopy, and Transmission Electron Microscopy

et al. 2015

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Biophysical properties including particle size distribution, integrity, and shape of whole virus vaccine particles at different stages in tick-borne encephalitis (TBE) vaccines formulation were analyzed by a new set of methods. Size-exclusion chromatography (SEC) was used as a conservative sample preparation for vaccine particle fractionation and gas-phase electrophoretic mobility macromolecular analyzer (GEMMA) for analyzing electrophoretic mobility diameters of isolated TBE virions. The derived particle diameter was then correlated with molecular weight. The diameter of the TBE virions determined after SEC by GEMMA instrumentation was 46.8 ± 1.1 nm. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) were implemented for comparison purposes and to gain morphological information on the virion particle. Western blotting (Dot Blot) as an immunological method confirmed biological activity of the particles at various stages of the developed analytical strategy. AFM and TEM measurements revealed higher diameters with much higher SD for a limited number of virions, 60.4 ± 8.5 and 53.5 ± 5.3 nm, respectively. GEMMA instrumentation was also used for fractionation of virions with specifically selected diameters in the gas-phase, which were finally collected by means of an electrostatic sampler. At that point (i.e., after particle collection), AFM and TEM showed that the sampled virions were still intact, exhibiting a narrow size distribution (i.e., 59.8 ± 7.8 nm for AFM and 47.5 ± 5.2 nm for TEM images), and most importantly, dot blotting confirmed immunological activity of the collected samples. Furthermore dimers and virion artifacts were detected, too.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%