Nanopartículas de prata biossintetizadas por Mikania glomerata Sprengel inibem o crescimento de Candida albicans e Staphylococcus aureus

Abstract

Introduction:In recent years there has been an increase in cases related to infection by Candida spp. and Staphylococcus spp., as well as the appearance of strains resistant to conventional antibiotics. Nanoparticle biosynthesis consists of the reduction of a metal ion by compounds of natural origin as secondary metabolites of plants and organisms, being the most indicated form because it presents less toxicity when compared to the chemical synthesis. In this way, the biological synthesis is an alternative to obtain new active agents for the treatment of microbial infections. Objective: Synthesize silver nanoparticles from the aqueous extract of Mikania glomerata Sprengel and evaluate possible microbicidal and cytotoxic activity. Material and Methods: For the synthesis of the silver nanoparticles (AgNPs) an aqueous extract of the leaves of Mikania glomerata plus a solution of silver nitrate was used. AgNPs synthesized was evaluated by UV-vis spectrophotometer and FAAS. Furthermore, antimicrobial activity was evaluated against strains of Candida albicans and Staphylococcus aureus and cytotoxicity activity against HeLa and Vero cell lines. Results: AgNPs are shown to be more efficient in combating Candida albicans and Staphylococcus aureus strains when compared to the pure administered extract. Up to the concentration of 100 mg/mL of the pure aqueous extract no inhibitory effect was observed on both microorganisms. However when the strains were in contact with AgNPs, the inhibitory concentration was 0.006 mg/mL and 0.1 mg/mL for S. aureus and C. albicans, respectively. The cytotoxic effect on the cells behaves in a dose-dependent manner, presenting greater cytotoxic potential against the HeLa cancer cell line. Conclusion: Thus, these results are promising and contribute to research related to the production of drugs using plant extract and metals. The AgNPs synthesized presented the antimicrobial potential against C. albicans and S. aureus, in addition to low activity against normal cells, indicating their reliability for application of AgNPs as an alternative form of treatment.Contribuição dos autores: BK coleta, delineamento do estudo, elaboração do manuscrito e redação do manuscrito, AHUY coleta, delineamento do estudo, elaboração do manuscrito e redação do manuscrito, NBD coleta, delineamento do estudo, elaboração do manuscrito e redação do manuscrito, MN orientação do projeto, discussão dos achados, delineamento do estudo, elaboração do manuscrito.

Evaluation of the antimicrobial activity of silver nanoparticles biosynthesized from the aqueous extract of Schinus terebinthifolius Raddi leaves

“…Regarding the antifungal activity, the nanoparticles showed superior efficacy in relation to the extract, being equally effective against C. albicans and C. glabrata , whereas the extract had no effect against C. glabrata . Similar results were obtained by Kischkel et al., who reported that silver nanoparticles biosynthesized from the leaf extract of Mikania glomerata Sprengel exhibited antifungal effect, whereas the extract was inactive 65 . According to Dutta et al., silver nanoparticles cause damage to the membrane and fungal wall, increasing the permeability of nanoparticles, leading to an increase in ROS and extravasation of intracellular components 66 …”

“…Similar results were obtained by Kischkel et al, who reported that silver nanoparticles biosynthesized from the leaf extract of Mikania glomerata Sprengel exhibited antifungal effect, whereas the extract was inactive. 65…”