Nanoparticles induce the biosynthesis and activity of the new possible therapeutic proteinase source, Talaromyces purpureogenus KJ584844

Negm, Sally; El-Metwally, Mohammad Magdy; Saber, WesamEldin I. A.; Abo-Neima, Sahar E.; Moustafa, Mahmoud; El‐kott, Attalla F.

doi:10.32604/biocell.2021.012011

Cited by 5 publications

(9 citation statements)

References 20 publications

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“…Nanotechnology has had a significant impact on several high-tech businesses in recent years, and it has been demonstrated that it has an impact on many microbial species, as well 1 .…”

Section: Discussionmentioning

confidence: 99%

“…These porous and agglomerated CNPs have been considered key phenomena for the synthesis of novel CNPs, hence maximizing their usefulness as antibiological phytosynthesized nanomaterials in biomedical and agricultural applications, where the porous nature can effectively adsorb harmful chemicals and antagonize the pathogens 2 , 33 . In contrast to bulk materials, which have lower porosity, nanoparticles with high porosity have a greater specific exterior area and high reaction activity 1 .…”

Section: Discussionmentioning

confidence: 99%

“…Recent advances in the synthesis of diverse nano-molecules of varied sizes, shapes, and functionalities have established nanotechnology as an essential technology in a variety of disciplines 1 . Thus, nanotechnology has been revolutionizing almost all realms in the life sciences and many high-technology industries 2 .…”

Section: Introductionmentioning

confidence: 99%

“…Nanoparticles, in contrast to bulk materials, have a greater specific exterior area and a high reaction activity. Thus, have been demonstrated to impact many living cells, including microbial ones 1 .…”

Section: Introductionmentioning

confidence: 99%

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An innovative green synthesis approach of chitosan nanoparticles and their inhibitory activity against phytopathogenic Botrytis cinerea on strawberry leaves

El‐Naggar

Saber

Zweil

et al. 2022

Sci Rep

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Green synthesis is a newly emerging field of nanobiotechnology that offers economic and environmental advantages over traditional chemical and physical protocols. Nontoxic, eco-friendly, and biosafe materials are used to implement sustainable processes. The current work proposes a new biological-based strategy for the biosynthesis of chitosan nanoparticles (CNPs) using Pelargonium graveolens leaves extract. The bioconversion process of CNPs was maximized using the response surface methodology. The best combination of the tested parameters that maximized the biosynthesis process was the incubation of plant extract with 1.08% chitosan at 50.38 °C for 57.53 min., yielding 9.82 ± 3 mg CNPs/mL. Investigation of CNPs by SEM, TEM, EDXS, zeta potential, FTIR, XRD, TGA, and DSC proved the bioconversion process's success. Furthermore, the antifungal activity of the biosynthesized CNPs was screened against a severe isolate of the phytopathogenic Botrytis cinerea. CNPs exerted efficient activity against the fungal growth. On strawberry leaves, 25 mg CNPs/mL reduced the symptoms of gray mold severity down to 3%. The higher concentration of CNPs (50 mg/mL) was found to have a reverse effect on the infected area compared with those of lower concentrations (12.5 and 25 mg CNPs/mL). Therefore, additional work is encouraged to reduce the harmful side effects of elevated CNPs concentrations.

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“…Nanotechnology has had a significant impact on several high-tech businesses in recent years, and it has been demonstrated that it has an impact on many microbial species, as well 1 .…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An innovative green synthesis approach of chitosan nanoparticles and their inhibitory activity against phytopathogenic Botrytis cinerea on strawberry leaves

El‐Naggar

Saber

Zweil

et al. 2022

Sci Rep

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“…NH 4 Cl was the best inorganic nitrogen source for AA production by the candidate bacterium. This may be back to its simple structure, which does not require a complicated biological metabolism for its assimilation (El-Metwally et al, 2016;Negm et al, 2021). The same could be applied to KH 2 PO 4 , which represents an initial source of P during the early stage of fermentation since inorganic phosphorus positively affects microbial growth (Miettinen et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Innovative Artificial-Intelligence- Based Approach for the Biodegradation of Feather Keratin by Bacillus paramycoides, and Cytotoxicity of the Resulting Amino Acids

et al. 2021

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The current study reported a new keratinolytic bacterium, which was characterized as Bacillus paramycoides and identified by 16S rRNA, and the sequence was then deposited in the GenBank (MW876249). The bacterium was able to degrade the insoluble chicken feather keratin (CFK) into amino acids (AA) through the keratinase system. The statistical optimization of the biodegradation process into AA was performed based on the Plackett–Burman design and rotatable central composite design (RCCD) on a simple solid-state fermentation medium. The optimum conditions were temperature, 37°C, 0.547 mg KH2PO4, 1.438 mg NH4Cl, and 11.61 days of incubation. Innovatively, the degradation of the CFK process was modeled using the artificial neural network (ANN), which was better than RCCD in modeling the biodegradation process. Differentiation of the AA by high-performance liquid chromatography (HPLC) revealed the presence of 14 AA including essential and non-essential ones; proline and aspartic acids were the most dominant. The toxicity test of AA on the HepG2 cell line did not show any negative effect either on the cell line or on the morphological alteration. B. paramycoides ZW-5 is a new eco-friendly tool for CFK degradation that could be optimized by ANN. However, additional nutritional trials are encouraged on animal models.

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Innovative biosynthesis, artificial intelligence-based optimization, and characterization of chitosan nanoparticles by Streptomyces microflavus and their inhibitory potential against Pectobacterium carotovorum

El‐Naggar

Bashir

Rabei

et al. 2022

Sci Rep

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Microbial-based strategy in nanotechnology offers economic, eco-friendly, and biosafety advantages over traditional chemical and physical protocols. The current study describes a novel biosynthesis protocol for chitosan nanoparticles (CNPs), employing a pioneer Streptomyces sp. strain NEAE-83, which exhibited a significant potential for CNPs biosynthesis. It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964). CNPs were characterized by SEM, TEM, EDXS, zeta potential, FTIR, XRD, TGA, and DSC. CNPs biosynthesis was maximized using a mathematical model, face-centered central composite design (CCFCD). The highest yield of CNPs (9.41 mg/mL) was obtained in run no. 27, using an initial pH of 5.5, 1% chitosan, 40 °C, and a 12 h incubation period. Innovatively, the artificial neural network (ANN), was used for validating and predicting CNPs biosynthesis based on the trials data of CCFCD. Despite the high precision degree of both models, ANN was supreme in the prediction of CNPs biosynthesis compared to CCFCD. ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE). CNPs biosynthesized by Streptomyces microflavus strain NEAE-83 showed in-vitro antibacterial activity against Pectobacterium carotovorum, which causes the potato soft rot. These results suggested its potential application for controlling the destructive potato soft rot diseases. This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.

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Nanoparticles induce the biosynthesis and activity of the new possible therapeutic proteinase source, Talaromyces purpureogenus KJ584844

Cited by 5 publications

References 20 publications

An innovative green synthesis approach of chitosan nanoparticles and their inhibitory activity against phytopathogenic Botrytis cinerea on strawberry leaves

An innovative green synthesis approach of chitosan nanoparticles and their inhibitory activity against phytopathogenic Botrytis cinerea on strawberry leaves

Innovative Artificial-Intelligence- Based Approach for the Biodegradation of Feather Keratin by Bacillus paramycoides, and Cytotoxicity of the Resulting Amino Acids

Innovative biosynthesis, artificial intelligence-based optimization, and characterization of chitosan nanoparticles by Streptomyces microflavus and their inhibitory potential against Pectobacterium carotovorum

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