Nanoparticles in the lungs of old mice: Pulmonary inflammation and oxidative stress without procoagulant effects

Luyts, Katrien; Broucke, Sofie Van Den; Hemmeryckx, Bianca; Poels, Katrien; Scheers, Hans; Casas, Lidia; Vanoirbeek, Jeroen; Nemery, Benoît; Hoet, Peter

doi:10.1016/j.scitotenv.2018.06.301

Cited by 14 publications

(10 citation statements)

References 43 publications

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“…Moderate to severe inflammation and alveolar wall devastation with moderate interstitial inflammation were also illustrated by another investigation [23] after 14 days of intraperitoneal injection with 1, 10 and 100 mg/kg ZnO NPs. An obvious alveolar inflammation was also reported [62] after eight weeks of the last dose of intratracheal instillation of ZnO NPs. In comparison with the control, the sections of testes in G1 , G2 , and G3 (Figure9-B,C, and D) showed severe damages of the testicular parenchyma, disorganized and severely necrotic seminiferous tubules, and macrovacuolation of germ cells, which may be related to the inflammatory response and explains the above mentioned significant decrease in testes weight index .…”

Section: Histopathological Studymentioning

confidence: 71%

Evaluation of the Toxicological Effects of Zinc Oxide Nanoparticles in Albino Male Mice

Razooki¹,

Rabee

2020

eijs

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The acute and sub chronic toxicity effects of 25.16 nm intraperitoneally- injected zinc oxide nanoparticles (ZnO NPs) were evaluated. Albino male mice were exposed to three different doses (25, 50 ,and 100 mg/kg ), depending on the value of calculated LD50, for 2 and 4 weeks . Considerable changes in organ indexes were shown with a good relevance to the illustrated histopathological effects which ranged from multiple hemorrhagic foci in liver, mild swelling and dilatation in kidney tubules, thickening of intestinal villi, moderate interstitial pneumonia, especially with the high dose , and sever necrosis of seminiferous tubules in testes of all studied groups. Significant changes in both hematological and biochemical parameters as well as thyroid hormones were observed with a considerable increase in the levels of antioxidant enzymes, in dose and exposure time dependent manner. The highest accumulated Zn mean values were recorded in the small intestine, kidney, liver, and spleen, respectively, followed by testes , heart , lung , and brain. These values followed the same order of the dose dependent manner, which explains the adverse effects that were recorded. This study proved the ability of using organ indexes as good tools side by side with the biochemical indicators to explain the histopathological changes. This study also revealed some histopathological effects that were not previously recorded as a toxicological effect of ZnO NPs in animal models.

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Section: Histopathological Studymentioning

confidence: 71%

Evaluation of the Toxicological Effects of Zinc Oxide Nanoparticles in Albino Male Mice

Razooki¹,

Rabee

2020

eijs

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“…Pulmonary oxidative stress is clearly elevated by MWCNT aspiration (41)(42)(43); however, we did not assess the contribution of oxidative stress to the systemic in ammatory spill-over in this study. The biological pathways underlying nanomaterialmediated respiratory pathology seem, at least on the surface, to mirror those of air pollution exposures: activation of in ammatory signaling, oxidative stress, enzyme activation and ECM remodeling (43)(44)(45). However the impact of MWCNT inhalation exposure outside the lung, while evident in many studies, remains an incompletely understood phenomenon.…”

Section: Discussionmentioning

confidence: 90%

“…Local cells produce MMP-9 when stimulated, and additional generation may occur from immune cells recruited to the lung microenvironment in response to insult; importantly, reactive oxygen species alone can activate constitutive MMPs (40). Pulmonary oxidative stress is clearly elevated by MWCNT aspiration (41)(42)(43); however, we did not assess the contribution of oxidative stress to the systemic in ammatory spill-over in this study. The biological pathways underlying nanomaterialmediated respiratory pathology seem, at least on the surface, to mirror those of air pollution exposures: activation of in ammatory signaling, oxidative stress, enzyme activation and ECM remodeling (43)(44)(45).…”

Section: Discussionmentioning

confidence: 93%

Role of Matrix Metalloproteinases in Multiwalled Carbon Nanotube-mediated Pulmonary and Systemic Inflammatory Activation

Young¹,

Mostovenko²,

Denson³

et al. 2021

Preprint

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Background: Multiwalled carbon nanotubes (MWCNT) are an increasingly utilized engineered nanomaterial that pose the potential for significant risk of exposure-related health outcomes. The mechanism(s) underlying MWCNT-induced toxicity to extrapulmonary sites are still being defined. MWCNT-induced serum-borne bioactivity appears to dysregulate systemic endothelial cell function. The serum compositional changes after MWCNT exposure have been identified as a surge of fragmented endogenous peptides, likely derived from matrix metalloproteinase (MMP) activity. In the present study, we utilize a broad-spectrum MMP inhibitor, Marimastat, along with a previously described oropharyngeal aspiration model of MWCNT administration to investigate the role of MMPs in MWCNT-derived serum peptide generation and endothelial bioactivity. Results: C57BL/6 mice were treated with Marimastat or vehicle by oropharyngeal aspiration 1h prior to MWCNT treatment. Pulmonary neutrophil infiltration and total bronchoalveolar lavage fluid protein increased in a dose-dependent manner, independent of MMP blockade. The lung cytokine profile similarly increased following MWCNT exposure for major inflammatory markers (IL-1β, IL-6, and TNFα), with minimal impact from MMP inhibition. However, serum peptidomic analysis revealed differential peptide compositional profiles, with MMP blockade abrogating MWCNT-derived serum peptide fragments. The serum, in turn, exhibited differential potency in terms of inflammatory bioactivity when incubated with primary murine cerebrovascular endothelial cells. Serum from MWCNT-treated mice led to inflammatory responses in endothelial cells that were significantly blunted with serum from Marimastat-treated mice. Conclusion: Thus, MWCNT exposure induced pulmonary inflammation that was largely independent of MMP activity, but generated circulating bioactive peptides through predominantly MMP-dependent pathways. This MWCNT-induced lung-derived bioactivity caused pathological consequences of endothelial inflammation and barrier disruption.

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“…It should be noted that the administration route largely determines the distribution pattern and further target organ toxicity of NMs in the body. For example, intravenous and intraperitoneal injection of ZnO NPs leads to their deposition in the liver, spleen, and kidneys along with the blood circulation [85,86], while nano-ZnO administered through intratracheal instillation mainly cause significant pulmonary inflammation in mice [87]. The physical and chemical properties are another important factor affecting the biological distribution of NMs.…”

Section: Distribution and Metabolism Of Nms In Vivomentioning

confidence: 99%

“…ALT and AST increased in the liver induction of autophagy accumulation of vacuolization LC3 ↑ [ 84 ] Graphene nanoplatelets 3 ~ 4 bare ICR mice 6 Weeks old) intratracheal instillation 2.5, 5 1, 7, 14, and 28 days 1. hyperplasia and hemorrhage 2. inflammatory response in the lung blockade of autophaic flux LC3 ↑ P62 ↑ [ 85 ] PAMAM NPs _ bare male Balb/c mice (6–10 weeks old) intratracheal administration 50 24 h lung inflammation and changed the lung elastance induction of autophagy LC3 ↑ [ 86 ] SWCNTs – COOH-CNT PABS-CNT PEG-CNT male Balb/c mice(6–8 weeks old) intratracheal administration 15 24 h 1. Acute pulmonary inflammation 2. severe lung edema induction of autophagy autophagosomes accumulation LC3↑ [ 87 ] CdTe QDs 4.08 bare male Balb/c mice (8–10 weeks old) intravenous injection 8 and 16 nmol/kg 24 h 1. increase in uric acid, creatinine and BUN 2. UPR and ER-phagy in the kidney and liver induction of autophagy LC3↑ [ 88 ] Fe 3 O 4 NPs 15 ~ 20 PLGA NIH mice intraperitoneal injection 10 2, 4, 8, 10 and 12 days extensive accumulation of autophagosome in the kidney and spleen induction of autophagy autophagosomes accumulation LC3 ↑ [ 89 ] MWCNTs 10 ~ 12 bare male Wistar rats (200–220 g) intraperitoneal injection 2.5 once per day for 14 days decrease...…”

Section: Introductionmentioning

confidence: 99%

Nanomaterial-mediated autophagy: coexisting hazard and health benefits in biomedicine

Feng

Zhang

et al. 2020

Part Fibre Toxicol

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Background Widespread biomedical applications of nanomaterials (NMs) bring about increased human exposure risk due to their unique physicochemical properties. Autophagy, which is of great importance for regulating the physiological or pathological activities of the body, has been reported to play a key role in NM-driven biological effects both in vivo and in vitro. The coexisting hazard and health benefits of NM-mediated autophagy in biomedicine are nonnegligible and require our particular concerns. Main body We collected research on the toxic effects related to NM-mediated autophagy both in vivo and in vitro. Generally, NMs can be delivered into animal models through different administration routes, or internalized by cells through different uptake pathways, exerting varying degrees of damage in tissues, organs, cells, and organelles, eventually being deposited in or excreted from the body. In addition, other biological effects of NMs, such as oxidative stress, inflammation, necroptosis, pyroptosis, and ferroptosis, have been associated with autophagy and cooperate to regulate body activities. We therefore highlight that NM-mediated autophagy serves as a double-edged sword, which could be utilized in the treatment of certain diseases related to autophagy dysfunction, such as cancer, neurodegenerative disease, and cardiovascular disease. Challenges and suggestions for further investigations of NM-mediated autophagy are proposed with the purpose to improve their biosafety evaluation and facilitate their wide application. Databases such as PubMed and Web of Science were utilized to search for relevant literature, which included all published, Epub ahead of print, in-process, and non-indexed citations. Conclusion In this review, we focus on the dual effect of NM-mediated autophagy in the biomedical field. It has become a trend to use the benefits of NM-mediated autophagy to treat clinical diseases such as cancer and neurodegenerative diseases. Understanding the regulatory mechanism of NM-mediated autophagy in biomedicine is also helpful for reducing the toxic effects of NMs as much as possible.

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Nanoparticles in the lungs of old mice: Pulmonary inflammation and oxidative stress without procoagulant effects

Cited by 14 publications

References 43 publications

Evaluation of the Toxicological Effects of Zinc Oxide Nanoparticles in Albino Male Mice

Evaluation of the Toxicological Effects of Zinc Oxide Nanoparticles in Albino Male Mice

Role of Matrix Metalloproteinases in Multiwalled Carbon Nanotube-mediated Pulmonary and Systemic Inflammatory Activation

Nanomaterial-mediated autophagy: coexisting hazard and health benefits in biomedicine

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