Nanoparticles functionalised with recombinant single chain Fv antibody fragments (scFv) for the magnetic resonance imaging of cancer cells

Vigor, Kim; Kyrtatos, Panagiotis G.; Minogue, Shane; Al‐Jamal, Khuloud T.; Kogelberg, Heide; Tolner, Berend; Kostarelos, Kostas; Begent, R. H. J.; Pankhurst, Quentin A.; Lythgoe, Mark F.; Chester, Kerry

doi:10.1016/j.biomaterials.2009.10.036

Cited by 68 publications

(49 citation statements)

References 44 publications

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“…In tumor imaging, antibody-SPIO conjugates were able to be used to successfully image numerous surface markers overexpressed in tumor vessels and cells, such transferrin receptor [115], folic acid receptor [116], VEGF [117], RGD [118], α ν β 3 -integrin as angiogenesis marker [119], Her2/neu (cerb B-2) tyrosinase receptor [114], uMUC-1 [120], LHRH-R [121], EGFR [122], CEA [123], CEACAM5 ( • " Fig. 5) [124], CXCR4 [125], CD20 [126] or B220 + (B-cell lymphomas) [127] with the help of T2w or T2*w MRI.…”

Section: Tumor Imagingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticles in Biomedicine: Applications and Developments in Diagnostics and Therapy

Ittrich¹,

Peldschus²,

Raabe³

et al. 2013

Fortschr Röntgenstr

124

101

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Superparamagnetic iron oxide nanoparticles (SPIO) can be used to image physiological processes and anatomical, cellular and molecular changes in diseases. The clinical applications range from the imaging of tumors and metastases in the liver, spleen and bone marrow, the imaging of lymph nodes and the CNS, MRA and perfusion imaging to atherosclerotic plaque and thrombosis imaging. New experimental approaches in molecular imaging describe undirected SPIO trapping (passive targeting) in inflammation, tumors and associated macrophages as well as the directed accumulation of SPIO ligands (active targeting) in tumor endothelia and tumor cells, areas of apoptosis, infarction, inflammation and degeneration in cardiovascular and neurological diseases, in atherosclerotic plaques or thrombi. The labeling of stem or immune cells allows the visualization of cell therapies or transplant rejections. The coupling of SPIO to ligands, radio- and/or chemotherapeutics, embedding in carrier systems or activatable smart sensor probes and their externally controlled focusing (physical targeting) enable molecular tumor therapies or the imaging of metabolic and enzymatic processes. Monodisperse SPIO with defined physicochemical and pharmacodynamic properties may improve SPIO-based MRI in the future and as targeted probes in diagnostic magnetic resonance (DMR) using chip-based ?NMR may significantly expand the spectrum of in vitro analysis methods for biomarker, pathogens and tumor cells. Magnetic particle imaging (MPI) as a new imaging modality offers new applications for SPIO in cardiovascular, oncological, cellular and molecular diagnostics and therapy. Key Points: Citation Format:

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Section: Tumor Imagingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticles in Biomedicine: Applications and Developments in Diagnostics and Therapy

Ittrich¹,

Peldschus²,

Raabe³

et al. 2013

Fortschr Röntgenstr

124

101

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“…Vigor et al 54 proposed that SPION could be used for the targeting and imaging of cancer cells if it could be functionalized with recombinant single-chain Fv antibody fragments (scFv). They generated antibody-functionalized (abf) SPION using a scFv specific for CEA.…”

Section: Using Spion In Cancer Imaging Applicationsmentioning

confidence: 99%

Superparamagnetic iron oxide nanoparticles for MR imaging of pancreatic cancer: Potential for early diagnosis through targeted strategies

Zhang¹,

Zou²,

Chen³

et al. 2015

Asia-Pac J Clncl Oncology

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Superparamagnetic iron oxide nanoparticles (SPION)-based magnetic resonance imaging is a powerful, noninvasive tool in biomedical imaging. The recent embedding of SPIO in nanoencapsulations that had different controllable surface properties has now made it possible to use SPIO in the imaging of metabolic processes. The two major issues to realize maximized and selective SPIO cancer targeting are the minimization of macrophage uptake and the preferential binding to cancerous cells over healthy neighbor cells. The utility of SPIO has been shown in clinical applications using a series of marketed SPION-based contrast agents. Applications have ranged from detecting inflammatory diseases to the specific identification of cell surface markers expressed on tumors. This review focuses on iron-oxide-based nanoparticles, to include the physiochemical properties of SPION surface engineering and its synthetic methods as well as SPIO imaging applications and specifically targeted SPIO conjugates (e.g. targeted probes) for labeling cancerous, cell-surface molecules. As a specific application of this technology, we discuss its use in the imaging of pancreatic duct adenocarcinoma in addition to its potential for use in early diagnosis through targeted strategies.

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“…Consideration of the number of reviews published on the subject in recent years, some of them regarding the very promising magnetic NPs [8][9][10][11][12], gives us an idea about this [13][14][15][16]. In most approaches, it is the material properties that have been investigated in search of new applications of NPs in biomedical fields.…”

Section: Introductionmentioning

confidence: 98%

Particle geometry, charge, and wettability

Delgado

López-Viota

Ramos

et al. 2014

Colloid and Interface Science in Pharmaceutical Research and Development

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Nanoparticles functionalised with recombinant single chain Fv antibody fragments (scFv) for the magnetic resonance imaging of cancer cells

Cited by 68 publications

References 44 publications

Superparamagnetic Iron Oxide Nanoparticles in Biomedicine: Applications and Developments in Diagnostics and Therapy

Superparamagnetic Iron Oxide Nanoparticles in Biomedicine: Applications and Developments in Diagnostics and Therapy

Superparamagnetic iron oxide nanoparticles for MR imaging of pancreatic cancer: Potential for early diagnosis through targeted strategies

Particle geometry, charge, and wettability

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