Nanoparticles from Gantrez-based conjugates for the oral delivery of camptothecin

Huarte, Judit; Espuelas, Socorro; Martínez-Ohárriz, Cristina; Irache, Juan Manuel

doi:10.1016/j.ijpx.2021.100104

Cited by 2 publications

(2 citation statements)

References 44 publications

(58 reference statements)

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“…This finding was confirmed by repeated experimentation. There could be various reasons for this phenomenon, but notably, PTX does have the lowest water solubility among the three drugs. ,− One possibility is that the strong hydrophobic bonds may slow the release of PTX from HeminNPs-PTX, reducing its effectiveness on 4T1 cells. ,, The cytotoxicity of HeminNPs-empty (as shown in Figure S9) was also evaluated. The HeminNPs-empty did not exhibit toxicity with 4T1, CT26, or ID8 cells, even after 72 h of incubation at a maximum concentration of 100 μM.…”

Section: Resultsmentioning

confidence: 99%

Hemin-Coated Nanoparticles as Drug Delivery Vehicles for Chemotherapeutic Agents in Cancer Therapy

Feng,

Yoo,

Nie

et al. 2023

ACS Appl. Nano Mater.

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Chemotherapy holds a prominent position in contemporary cancer treatment modalities; however, poor water solubility, rapid clearance, and unintended side effects remain significant challenges. To mitigate these issues, various nanoparticlebased delivery systems have been devised to improve the pharmacokinetic and pharmacodynamic profiles of chemotherapeutic drugs. While biocompatible, most of these drug delivery systems include materials that are foreign to the human body. Consequently, it is critical for these materials to be effectively removed from the body to prevent their potential toxicity. In this study, we introduce a nanoparticle-based system that utilizes only hemin, which is naturally found in the body, as an amphiphilic coating agent to facilitate the solubilization of hydrophobic drugs. Camptothecin (CPT), Paclitaxel (PTX), and Sorafenib (SOR) were each formulated into stable hemin-coated nanoparticles (HeminNPs). We demonstrate that hemin-coated CPT nanoparticles exhibit an improved safety profile and therapeutic efficacy compared with the administration of CPT alone in a murine tumor model of triple-negative breast cancer. Consequently, the utilization of HeminNPs demonstrates potential in reducing off-target toxicity and amplifying the therapeutic effectiveness of cancer treatments, which may ultimately contribute to improved treatment outcomes for cancer patients.

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Section: Resultsmentioning

confidence: 99%

Hemin-Coated Nanoparticles as Drug Delivery Vehicles for Chemotherapeutic Agents in Cancer Therapy

Feng,

Yoo,

Nie

et al. 2023

ACS Appl. Nano Mater.

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“…MN arrays were prepared using poly(methyl-vinyl-ether-co-maleic anhydride) (Gantrez ® AN-119) (Mw = 200 kDa), whose physicochemical properties were characterized before [13] The procedure is illustrated in Figure 1. To prepare the MN arrays, 50 mg of a solution containing 30% (w/v) Gantrez AN-119 and 5 µg of the rLTB protein was poured into silicone micromolds.…”

Section: Polymeric Mn Arrays Formulationmentioning

confidence: 99%

Immune Response after Skin Delivery of a Recombinant Heat-Labile Enterotoxin B Subunit of Enterotoxigenic Escherichia coli in Mice

et al. 2022

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Enterotoxigenic Escherichia coli (ETEC) infections have been identified as a major cause of acute diarrhoea in children in developing countries, associated with substantial morbidity and mortality rates. Additionally, ETEC remains the most common cause of acute diarrhea of international travellers to endemic areas. The heat-labile toxin (LT) is a major virulence factor of ETEC, with a significant correlation between the presence of antibodies against LT and protection in infected patients. In the present work, we constructed a recombinant LTB unit (rLTB) and studied the capacity of this toxoid incorporated in microneedles (rLTB-MN) to induce a specific immune response in mice. MN were prepared from aqueous blends of the polymer Gantrez AN® [poly (methyl vinyl ether-co-maleic anhydride)], which is not cytotoxic and has been shown to possess immunoadjuvant properties. The mechanical and dissolution properties of rLTB-MNs were evaluated in an in vitro Parafilm M® model and in mice and pig skin ex vivo models. The needle insertion ranged between 378 µm and 504 µm in Parafilm layers, and MNs fully dissolved within 15 min of application inside porcine skin. Moreover, female and male BALB/c mice were immunized through ear skin with one single dose of 5 μg·rLTB in MNs, eliciting significant fecal anti-LT IgA antibodies, higher in female than in male mice. Moreover, we observed an enhanced production of IL-17A by spleen cells in the immunized female mice, indicating a mucosal non-inflammatory and neutralizing mediated response. Further experiments will now be required to validate the protective capacity of this new rLTB-MN formulation against this deadly non-vaccine-preventable disease.

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Nanoparticles from Gantrez-based conjugates for the oral delivery of camptothecin

Cited by 2 publications

References 44 publications

Hemin-Coated Nanoparticles as Drug Delivery Vehicles for Chemotherapeutic Agents in Cancer Therapy

Hemin-Coated Nanoparticles as Drug Delivery Vehicles for Chemotherapeutic Agents in Cancer Therapy

Immune Response after Skin Delivery of a Recombinant Heat-Labile Enterotoxin B Subunit of Enterotoxigenic Escherichia coli in Mice

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