Nanoparticles for Stem‐Cell Engineering

Jabbari, Esmaiel

doi:10.1002/9781118540640.ch9

Cited by 1 publication

(1 citation statement)

References 233 publications

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“…When conjugated with biomolecular targeting ligands, Raman reporter-labelled gold (Au) nanoparticles can be used to detect specific molecules with high specificity and sensitivity [19, 21–23]. SERS detection produces a sharp, fingerprint-like spectral pattern that is distinct from other interference patterns in a complex biological environment.…”

Section: Introductionmentioning

confidence: 99%

Novel surface-enhanced Raman scattering-based assays for ultra-sensitive detection of human pluripotent stem cells

Han

Qian

et al. 2016

Biomaterials

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Human pluripotent stem cells (hPSCs) are a promising cell source for regenerative medicine, but their derivatives need to be rigorously evaluated for residual stem cells to prevent teratoma formation. Here, we report the development of novel surface-enhanced Raman scattering (SERS)-based assays that can detect trace numbers of undifferentiated hPSCs in mixed cell populations in a highly specific, ultra-sensitive, and time-efficient manner. By targeting stem cell surface markers SSEA-5 and TRA-1-60 individually or simultaneously, these SERS assays were able to identify as few as 1 stem cell in 106 cells, a sensitivity (0.0001%) which was ~2,000 to 15,000-fold higher than that of flow cytometry assays. Using the SERS assay, we demonstrate that the aggregation of hPSC-based cardiomyocyte differentiation cultures into 3D spheres significantly reduced SSEA-5+ and TRA-1-60+ cells compared with parallel 2D cultures. Thus, SERS may provide a powerful new technology for quality control of hPSC-derived products for preclinical and clinical applications.

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Section: Introductionmentioning

confidence: 99%