Nanoparticles-cell association predicted by protein corona fingerprints

Palchetti, Sara; Digiacomo, Luca; Pozzi, Daniela; Peruzzi, Giovanna; Micarelli, Elisa; Mahmoudi, Morteza; Caracciolo, Giulio

doi:10.1039/c6nr03898k

Cited by 76 publications

(58 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Remarkably, the identification of those importantly adsorbed proteins was found with several high-expression receptors of Hela cell membrane. 57 Figure 2 shows a scheme for the establishment of predictive model. From the model, biological responses can be predicted without further experiments and highly expressed receptors can be identified from the most influencing proteins for NP design.…”

Section: Protein Corona Applications Physiological Prediction Via Modmentioning

confidence: 99%

Protein corona: a new approach for nanomedicine design

Nguyen¹,

Lee²

2017

IJN

521

343

View full text Add to dashboard Cite

After administration of nanoparticle (NP) into biological fluids, an NP–protein complex is formed, which represents the “true identity” of NP in our body. Hence, protein–NP interaction should be carefully investigated to predict and control the fate of NPs or drug-loaded NPs, including systemic circulation, biodistribution, and bioavailability. In this review, we mainly focus on the formation of protein corona and its potential applications in pharmaceutical sciences such as prediction modeling based on NP-adsorbed proteins, usage of active proteins for modifying NP to achieve toxicity reduction, circulation time enhancement, and targeting effect. Validated correlative models for NP biological responses mainly based on protein corona fingerprints of NPs are more highly accurate than the models solely set up from NP properties. Based on these models, effectiveness as well as the toxicity of NPs can be predicted without in vivo tests, while novel cell receptors could be identified from prominent proteins which play important key roles in the models. The ungoverned protein adsorption onto NPs may have generally negative effects such as rapid clearance from the bloodstream, hindrance of targeting capacity, and induction of toxicity. In contrast, controlling protein adsorption by modifying NPs with diverse functional proteins or tailoring appropriate NPs which favor selective endogenous peptides and proteins will bring promising therapeutic benefits in drug delivery and targeted cancer treatment.

show abstract

Section: Protein Corona Applications Physiological Prediction Via Modmentioning

confidence: 99%

Protein corona: a new approach for nanomedicine design

Nguyen¹,

Lee²

2017

IJN

521

343

View full text Add to dashboard Cite

show abstract

“…Importantly, the protein corona underpins the biological identity and fate of the NPs, in stark contrast to that of the pristine NPs. It is this acquired biological identity that further dictates cell interactions, uptake, biodistribution, and clearance of the NPs in vivo …”

Section: Introductionmentioning

confidence: 99%

Plasma Proteome Association and Catalytic Activity of Stealth Polymer‐Grafted Iron Oxide Nanoparticles

Wang

Siddiqui

Gustafsson

et al. 2017

Small

View full text Add to dashboard Cite

Polyethylene glycol (PEG) is widely used as an antifouling and stealth polymer in surface engineering and nanomedicine. However, recent research has revealed adverse effects of bioaccumulation and immunogenicity following the administration of PEG, prompting this proteomic examination of the plasma protein coronae association with superparamagnetic iron oxide nanoparticles (IONPs) grafted with brushed PEG (bPEG) and an alternative, brushed phosphorylcholine (bPC). Using label-free quantitation by liquid chromatography tandem-mass spectrometry, this study determines protein abundances for the in vitro hard coronae of bare, bPC-, and bPEG-grafted IONPs in human plasma. This study also shows unique protein compositions in the plasma coronae of each IONP, including enrichment of coagulation factors and immunogenic complement proteins with bPEG, and enhanced binding of apolipoproteins with bPC. Functional analysis reveals that plasma protein coronae elevate the horseradish peroxidase-like activities of the bPC- and bPEG-IONPs by approximately twofold, an effect likely mediated by the diverse composition and physicochemical properties of the polymers as well as their associated plasma proteins. Taken together, these observations support the rational design of stealth polymers based on a quantitative understanding of the interplay between IONPs and the plasma proteome, and should prove beneficial for the development of materials for nanomedicine, biosensing, and catalysis.

show abstract

“…tissues and organs) or to promote tunable cellular interactions. Even tough recent research suggests a strong relationship between the nature of BC and the NP-cell association 13, 14, 30, 31 , its precise role in regulating cellular uptake and subcellular processes has not been identified yet. The central aim of this investigation was therefore to explore the role played by the BC on the cellular uptake and intracellular trafficking of NPs.…”

Section: Resultsmentioning

confidence: 99%

An apolipoprotein-enriched biomolecular corona switches the cellular uptake mechanism and trafficking pathway of lipid nanoparticles

et al. 2017

Self Cite

View full text Add to dashboard Cite

Following exposure to biological milieus (e.g. after systemic administration), nanoparticles (NPs) get covered by an outer biomolecular corona (BC) that defines many of their biological outcomes, such as the elicited immune response, biodistribution, and targeting capabilities. In spite of this, the BC role in regulating the cellular uptake and the subcellular trafficking properties of NPs has remained elusive. Here, we tackle this issue by employing multicomponent (MC) lipid NPs, human plasma (HP) and HeLa cells as models for nanoformulation, biological fluid, and target cell (respectively). By confocal fluorescence microscopy experiments and image correlation analyses, we quantitatively demonstrate that the BC promotes a neat switch of cell entry mechanism and subsequent intracellular trafficking, from macropinocytosis to clathrin-dependent endocytosis. Nano liquid chromatography tandem mass spectrometry identifies Apolipoproteins as the most abundant components of the BC tested here. Interestingly, this class of proteins target the LDL receptors, which are overexpressed in clathrin-enriched membrane domains. Our results highlight the crucial role of BC as an intrinsic trigger of specific NP-cell interactions and biological responses and set the basis for a rational exploitation of the BC for targeted delivery.

show abstract

Nanoparticles-cell association predicted by protein corona fingerprints

Cited by 76 publications

References 47 publications

Protein corona: a new approach for nanomedicine design

Protein corona: a new approach for nanomedicine design

Plasma Proteome Association and Catalytic Activity of Stealth Polymer‐Grafted Iron Oxide Nanoparticles

An apolipoprotein-enriched biomolecular corona switches the cellular uptake mechanism and trafficking pathway of lipid nanoparticles

Contact Info

Product

Resources

About