Nanoparticles as Nonviral Gene Delivery Vectors

Ragusa, Andrea; Garcı́a, Isabel; Penadés, Soledad

doi:10.1109/tnb.2007.908996

Cited by 73 publications

(60 citation statements)

References 134 publications

(117 reference statements)

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“…Taken into account this point, we selected chitosan nanoparticles, a non-viral vector, in the present study. Non-viral vectors include liposomes [7][8][9] , composites [10] , microspheres [11] , and nanoparticles [12,13] , but the cytotoxicity of the liposomes limits its application in vivo. Owing to its loose constitution, the constancy of composites is poor.…”

Section: Introductionmentioning

confidence: 99%

Expression of human insulin gene wrapped with chitosan nanoparticles in NIH3T3 cells and diabetic rats

Niu

Xie

et al. 2008

Acta Pharmacol Sin

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Aim: To study the expression of human insulin gene wrapped with chitosan nanoparticles in NIH3T3 cells and diabetic rats. Methods: pCMV.Ins, an expression plasmid of the human insulin gene, was constructed. In total, 100 μg pCMV.Ins wrapped with chitosan nanoparticles (chitosan-pCMV.Ins) was transfected to NIH3T3 cells and diabetes rats through lavage and coloclysis, respectively. The transfected cells were grown in Dulbecco's modified Eagle's medium, containing G418, for 72 h after transfection. The clones were selected and continued to grow in G418 medium for 24 d. The expression of human insulin was detected by immunohistochemistry. Human insulin in the culture medium of transfected cells was measured. Fasting blood glucose and plasma human insulin of diabetic rats were measured for 5 d after transfection. RT-PCR and Western blotting were performed to confirm the expression of the human insulin gene in diabetic rats. Results: Approximately 10% of NIH3T3 cells transfected by chitosan-pCMV.Ins expressed human insulin. Human insulin in the culture medium of NIH3T3 cells transfected by chitosan-pCMV.Ins significantly increased compared with that of the control group (P<0.01). Fasting blood glucose levels of the lavage group and the coloclysis group decreased significantly in 5 d (P<0.01) in comparison, while plasma insulin levels were much higher (P<0.01). The human insulin gene mRNA and human insulin were only detected in the lavage and the coloclysis groups. Conclusion: The human insulin gene can be transfected and expressed successfully by chitosanpCMV.Ins in NIH3T3 cells and diabetes rats, which indicates that chitosan is a promising, non-viral vector for gene expression.

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Section: Introductionmentioning

confidence: 99%

Expression of human insulin gene wrapped with chitosan nanoparticles in NIH3T3 cells and diabetic rats

Niu

Xie

et al. 2008

Acta Pharmacol Sin

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“…Prior publications have reviewed the benefits of NPs as delivery vehicles for plasmid DNA, [8,9] and the potential benefits of magnetofection [8,41] in: 1) facilitating the entry of the NPs into cells; 2) targeting NPs to selected sites in vivo; and 3) retaining NPs at selected sites. The NPs most often used for magnetofection have been iron oxides: magnetite and maghemite.…”

Section: Discussionmentioning

confidence: 99%

Novel Magnetic Hydroxyapatite Nanoparticles as Non‐Viral Vectors for the Glial Cell Line‐Derived Neurotrophic Factor Gene

Wang

Bohn

et al. 2009

Adv Funct Materials

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Nanoparticles (NPs) of synthetic hydroxyapatite (Hap) and natural bone mineral (NBM) are rendered magnetic by treatment with iron ions using a wet‐chemical process. The magnetic NPs (mNPs), which are about 300 nm in diameter, display superparamagnetic properties in a superconducting quantum interference device, with a saturation magnetization of about 30 emu g−1. X‐ray diffraction and transmission electron microscopy reveal that the magnetic properties of the NPs are the result of the hetero‐epitaxial growth of magnetite on the Hap and NBM crystallites. The mNPs display a high binding affinity for plasmid DNA in contrast to magnetite NPs which do not bind the plasmid well. The mHap and mNBM NPs result in substantial increases in the transfection of rat marrow‐derived mesenchymal stem cells with the gene for glial cell line‐derived neurotrophic factor (GDNF), with magnetofection compared to transfection in the absence of a magnet. The amount of GDNF recovered in the medium approaches therapeutic levels despite the small amount of plasmid delivered by the NPs.

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“…Non-viral vector includes liposome [23][24][25] , composite [26] , microsphere, and nanopar ticles, etc [27,28] , but the cytotoxicity of the bangosome limits its application in vivo. Owing to its loose constitution, the constancy of composites is poor.…”

Section: Discussionmentioning

confidence: 99%

Gene therapy for type 1 diabetes mellitus in rats by gastrointestinal administration of chitosan nanoparticles containing human insulin gene

Niu¹,

Xu²,

Dai³

et al. 2008

WJG

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AIM:To study the expression of human insulin gene in gastrointestinal tracts of diabetic rats. METHODS: pCMV.Ins, an expression plasmid of the human insulin gene, wrapped with chitosan nanoparticles, was transfected to the diabetic rats through lavage and coloclysis, respectively. Fasting blood glucose and plasma insulin levels were measured for 7 d. Reverse transcription polymerase chain reaction (RT-PCR) analysis and Western blot analysis were performed to confirm the expression of human insulin gene. RESULTS: Compared with the control group, the fasting blood glucose levels in the lavage and coloclysis groups were decreased significantly in 4 d (5.63 ± 0.48 mmol/L and 5.07 ± 0.37 mmol/L vs 22.12 ± 1.31 mmol/L, respectively, P < 0.01), while the plasma insulin levels were much higher (32.26 ± 1.81 μIU/mL and 32.79 ± 1.84 μIU/mL vs 14.23 ± 1.38 μIU/mL, respectively, P < 0.01). The human insulin gene mRNA and human insulin were only detected in the lavage and coloclysis groups. CONCLUSION: Human insulin gene wrapped with chitosan nanoparticles can be successfully transfected to rats through gastrointestinal tract, indicating that chitosan is a promising non-viral vector.

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Nanoparticles as Nonviral Gene Delivery Vectors

Cited by 73 publications

References 134 publications

Expression of human insulin gene wrapped with chitosan nanoparticles in NIH3T3 cells and diabetic rats

Expression of human insulin gene wrapped with chitosan nanoparticles in NIH3T3 cells and diabetic rats

Novel Magnetic Hydroxyapatite Nanoparticles as Non‐Viral Vectors for the Glial Cell Line‐Derived Neurotrophic Factor Gene

Gene therapy for type 1 diabetes mellitus in rats by gastrointestinal administration of chitosan nanoparticles containing human insulin gene

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