Nanoparticle tracking analysis as a process analytical tool for characterising magnetosome preparations

Fernández-Castañé, Alfred; Li, Hong; Joseph, Stephan; Ebeler, Moritz; Franzreb, Matthias; Bracewell, Daniel G.; Overton, Tim W.; Thomas, Owen R.T.

doi:10.1016/j.fbp.2021.03.013

Cited by 6 publications

(4 citation statements)

References 47 publications

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“…The precision, accuracy, and linearity of NTA analysis can vary based on instrumentation parameters, the properties of particle solutions (particle size, shape, concentration, etc. ), sample preparation, and user expertise. − In general, the disadvantage of NTA is its operation within a narrow concentration range, typically in the order of 10 8 to 10 9 nanoparticles/mL, as determined in previous reports. , Matrix effects and the uneven distribution of fluorescent molecules may compromise the precision and accuracy of converting fluorescent measurements into particle numbers. Furthermore, it is crucial that the fluorescent molecules remain tightly associated with the nanoparticles without significant leakage during the uptake study, a condition that we have confirmed in our case.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of Macrophage Uptake and Retention of Fluorescent Organosilica Nanoparticles: Implications for Nanoparticle Delivery and Therapeutics

Chou,

Chiu,

2024

ACS Appl. Nano Mater.

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This study investigates the uptake and retention of stable fluorescent organosilica nanoparticles by macrophages, which play a vital role in scavenging environmental nanoparticles and nanomedicines within the body. We used rhodamine 6G-loaded fluorescent organosilica nanoparticles (SiNP-R6G) synthesized from a thiol-functionalized organosilane precursor. Our primary objective was to establish a quantitative relationship between fluorescent measurements and nanoparticle tracking analysis, enabling the precise "counting" of nanoparticles taken up by macrophages under kinetic measurement conditions. Our kinetic study demonstrated a concentration-dependent, saturable internalization of nanoparticles in a model macrophage (RAW 264.7 cells), with a maximum uptake rate (V max ) of 7.9 × 10 4 nanoparticles per hour per cell. The estimated number concentration of nanoparticles for half-maximum uptake was approximately 0.8 trillion nanoparticles per milliliter, and a significant portion (∼80%) of internalized SiNP-R6G remained entrapped within the cells for 48 h, indicating the sustained particle retention capacity of macrophages. Our data further demonstrate that the internalized nanoparticles occupy more than 10% of the total cell volume, without inducing cytotoxic effects. This heightened capacity for nanoparticle uptake is attributed to intracellular vesicles capable of accommodating up to 50 densely packed, yet nonaggregated, nanoparticles within a single vesicle. In summary, our study underscores the effective development of a methodology for precise "counting" of cellular nanoparticle uptake in macrophages, providing valuable insights into the kinetics and retention capabilities of macrophages for nanoparticle-based drug delivery.

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Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of Macrophage Uptake and Retention of Fluorescent Organosilica Nanoparticles: Implications for Nanoparticle Delivery and Therapeutics

Chou,

Chiu,

2024

ACS Appl. Nano Mater.

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“…The demand for methods to evaluate NP size for continuous manufacturing and production/process analysis technology (PAT) are expected to grow (69,70). The NPs produced by microfluidic technologies have become the focus of research attention in recent years.…”

Section: Other Challengesmentioning

confidence: 99%

Current Status and Challenges of Analytical Methods for Evaluation of Size and Surface Modification of Nanoparticle-Based Drug Formulations

et al. 2022

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The present review discusses the current status and difficulties of the analytical methods used to evaluate size and surface modifications of nanoparticle-based pharmaceutical products (NPs) such as liposomal drugs and new SARS-CoV-2 vaccines. We identified the challenges in the development of methods for (1) measurement of a wide range of solid-state NPs, (2) evaluation of the sizes of polydisperse NPs, and (3) measurement of non-spherical NPs. Although a few methods have been established to analyze surface modifications of NPs, the feasibility of their application to NPs is unknown. The present review also examined the trends in standardization required to validate the size and surface measurements of NPs. It was determined that there is a lack of available reference materials and it is difficult to select appropriate ones for modified NP surface characterization. Research and development are in progress on innovative surface-modified NP-based cancer and gene therapies targeting cells, tissues, and organs. Next-generation nanomedicine should compile studies on the practice and standardization of the measurement methods for NPs to design surface modifications and ensure the quality of NPs.

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“…PAT not only helps in running a process within a specified design space but also broadens the design space [41]. Some broadly used PAT tools are UV-visible spectroscopy, Raman spectroscopy, infrared spectroscopy, two dimension (2D) fluorescence spectroscopy, mass spectroscopy, and nanoparticle tracking analysis (NTA) [42,43].…”

Section: Process Analytical Toolmentioning

confidence: 99%

Quality by design based development of nanostructured lipid carrier: a risk based approach

Alam

2022

Exploration of Medicine

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The aim of this review is to discuss the development of nanostructured lipid carrier (NLC) by the application of quality by design (QbD). QbD started with the evolution of the quality concept and slow adaptation of quality guidelines, which has now become a regulatory requirement. In this review, brief history and elements of QbD including risk assessment (RA) have been discussed followed by the design of experiments (DoEs) that acts as a tool to analyze the input whose variation can optimize the output with the desired goal. NLC is a versatile delivery system as researchers widely use it to administer therapeutics with different physicochemical properties. The surface of NLC can be modified, making it a suitable delivery system with targeting potential for therapeutics. Implementation of QbD provides a high-quality robust formulation that can consistently meet the patient’s requirement throughout its life cycle without compromising the safety and effectiveness of the drug and delivery system. This review discusses QbD concepts followed by the systematic development of NLC by the application of DoE. Process analytical technology (PAT) and six sigma concepts have also been included which can benefit in the development of optimized NLC.

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Nanoparticle tracking analysis as a process analytical tool for characterising magnetosome preparations

Cited by 6 publications

References 47 publications

Quantitative Analysis of Macrophage Uptake and Retention of Fluorescent Organosilica Nanoparticles: Implications for Nanoparticle Delivery and Therapeutics

Quantitative Analysis of Macrophage Uptake and Retention of Fluorescent Organosilica Nanoparticles: Implications for Nanoparticle Delivery and Therapeutics

Current Status and Challenges of Analytical Methods for Evaluation of Size and Surface Modification of Nanoparticle-Based Drug Formulations

Quality by design based development of nanostructured lipid carrier: a risk based approach

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