Nanoparticle oral absorption and its clinical translational potential

Kim, Kyoung Sub; Na, Kun; Bae, You Han

doi:10.1016/j.jconrel.2023.06.024

Cited by 4 publications

(1 citation statement)

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“…This system incorporates enterocytes, mucus-secreting HT29-MTX cells, and M cells [ 67 , 68 ]. M cells exhibit a notable capacity for transcytosis and have the ability to transport a diverse array of materials, encompassing nanoparticles as well [ 69 , 70 ]. A study carried out by Yuan et al [ 71 ] reported that up to 78% of the absorbed SLPs were conveyed into the systemic circulation through the lymphatic system, predominantly via the uptake by M cells.…”

Section: Resultsmentioning

confidence: 99%

Enhanced Cellular Uptake and Transport of Bovine Lactoferrin Using Pectin- and Chitosan-Modified Solid Lipid Nanoparticles

Yao,

Bunt,

Liu

et al. 2023

Pharmaceutics

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Aim: The aim of this project is to use pectin- and chitosan-modified solid lipid nanoparticles for bovine lactoferrin to enhance its cellular uptake and transport. Methods: Solid lipid particles containing bovine lactoferrin (bLf) were formulated through the solvent evaporation technique, incorporating stearic acid along with either chitosan or pectin modification. bLf cellular uptake and transport were evaluated in vitro using the human adenocarcinoma cell line Caco-2 cell model. Results and Discussion: The bLf-loaded SLPs showed no significant effect on cytotoxicity and did not induce apoptosis within the eight-hour investigation. The use of confocal laser scanning microscopy confirmed that bLf follows the receptor-mediated endocytosis, whereas the primary mechanism for the cellular uptake of SLPs was endocytosis. The bLf-loaded SLPs had significantly more cellular uptake compared to bLf alone, and it was observed that this impact varied based on the time, temperature, and concentration. Verapamil and EDTA were determined to raise the apparent permeability coefficients (App) of bLf and bLf-loaded SLPs. Conclusion: This occurred because they hindered efflux by interacting with P-glycoproteins and had a penetration-enhancing influence. These findings propose the possibility of an additional absorption mechanism for SLPs, potentially involving active transportation facilitated by the P-glycoprotein transporter in Caco-2 cells. These results suggest that SLPs have the potential to be applied as effective carriers to improve the oral bioavailability of proteins and peptides.

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