Nanoparticle-mediated interplay of chitosan and melatonin for improved wound epithelialisation

Blažević, Filip; Milekić, Tamara; Romić, Marieta Duvnjak; Juretić, Marina; Pepić, Ivan; Filipović-Grčić, Jelena; Lovrić, Jasmina; Hafner, Anita

doi:10.1016/j.carbpol.2016.03.074

“…Blažević et al developed melatonin-loaded lecithin/chitosan nanoparticles and and tested their effect on the wound epithelialisation in vitro [74]. Four different types of chitosan were tested for their biocompatibility and in vitro release of melatonin.…”

Section: Polymeric Nanoparticlesmentioning

confidence: 99%

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

Souto

¹

,

Ribeiro

²

,

Ferreira

³

et al. 2020

IJMS

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Burn wounds are highly debilitating injuries, with significant morbidity and mortality rates worldwide. In association with the damage of the skin integrity, the risk of infection is increased, posing an obstacle to healing and potentially leading to sepsis. Another limitation against healing is associated with antibiotic resistance mainly due to the use of systemic antibiotics for the treatment of localized infections. Nanotechnology has been successful in finding strategies to incorporate antibiotics in nanoparticles for the treatment of local wounds, thereby avoiding the systemic exposure to the drug. This review focuses on the most recent advances on the use of nanoparticles in wound dressing formulations and in tissue engineering for the treatment of burn wound infections.

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“…37 Similarly, in a recent study, HaCaT cells exposed to melatonin containing lecithin/chitosan NPs in a concentration of chitosan of 1.25-20 μg/mL for 2 hours showed no relevant cytotoxicity. 38 However, a signifi cant reduction in the cell viability of HaCaT cells was observed in the case of cells treated with NPs at a chitosan concentration of 20 μg/mL. 38 Chitosan-alginate NPs did not have a toxic effect on human monocytes but there was mild toxicity to skin keratinocytes at higher concentration of NPs.…”

Section: Chitosan Modified Poly(lactic-co-glycolic) Acid Nanoparticlementioning

confidence: 99%

“…39 A possible explanation for the chitosan NPs high biocompatibilty could be that chitosan is much more cytotoxic in a free soluble form than when it is incorporated into NPs, due to the fact that in the case of NPs, a signifi cant portion of the positive amino groups of chitosan are engaged in electrostatic interractions. 38,40 To confi rm PLGAChi NPs effi ciency in intracellular penetration, the cellular internalization of PLGAChi NPs conjugated with fl uorescein was investigated by fl uorescence microscopy. The results indicated significant differences in NPs uptake between the different cell lines used in this study.…”

Section: Chitosan Modified Poly(lactic-co-glycolic) Acid Nanoparticlementioning

confidence: 99%

“…30 The performance of a delivery system depends on the polymeric composition, the size and surface charge. 38,47 Therefore, smaller sizes of NPs and a positive zeta potential lead to a better internalisation inside cells due to the attractive interaction with the negatively charged cell membranes. 48,49 Hence, the size and zeta potential of the cureent NPs fabricated in our study are in favour of particle internalisation.…”

Section: Chitosan Modified Poly(lactic-co-glycolic) Acid Nanoparticlementioning

confidence: 99%

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Chitosan Modified Poly(lactic-Co-Glycolic) Acid Nanoparticles Interaction With Normal, Precancerous Keratinocytes and Dental Pulp Cells

Vîrlan¹,

Calenic²,

Cimpan³

et al. 2017

EDUJ

0

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Introduction: Nanoparticles (NPs) can carry molecules to different body tissues. Due to their controlled delivery properties, chitosan covered poly-lacto-co-glycolic NPs (PLGAChi NPs) could be used to deliver drugs to oral tissues for the treatment of dental diseases or in anticancer therapy. The aim of this study was to determine the uptake and cytotoxicity of PLGAChi NPs on different types of cells found in the oral cavity. Methodology: Normal oral keratinocytes (NOKs), precancerous keratinocytes (POE9i) and dental pulp cells (DPCs) were exposed for 12h and 24h to 20 g/mL and 200 g/mL PLGAChi NPs covalently tagged with fl uorescein. 3D organotypic tissues of oral mucosa were grown in vitro and exposed to 200g/mL PLGAChi NPs for 24h. Results: Both normal and premalignant oral mucosa cells (NOKs and POE9i) displayed uptake of PLGAChiNPs in a time and concentration-dependent manner, both in 2D and 3D models. A higher and more rapid uptake of PLGAChi NPs by precancerous cell line POE9i was observed when compared to NOKs. Interestingly, DPCs did not display internalized PLGAChi NPs, even at the highest concentration of 200 g/mL. Conclusion: Chitosan-coated PLGAChi NPs proved to be able to cross the cellular membrane of oral keratinocytes, in 2D as well as in 3D cultures. The polymeric NPs used in the present study seem not to be suitable for applications that require NPs uptake by DPCs, as no evidence of uptake in these cells was found in this study. The fi nding that PLGAChi NPs showed signifi cant internalization by human keratinocytes indicate that they could be used for drug delivery purposes to oral mucosa. Keywords: chitosan, PLGAChi, nanoparticles, oral keratinocytes, dental pulp. ABSTRACT

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“…38 However, a signifi cant reduction in the cell viability of HaCaT cells was observed in the case of cells treated with NPs at a chitosan concentration of 20 μg/mL. 38 Chitosan-alginate NPs did not have a toxic effect on human monocytes but there was mild toxicity to skin keratinocytes at higher concentration of NPs. 29 Moreover, chitosan and PLGA NPs loaded with chlorexidine dihydrochloride in vitro toxicity evaluation on human gingival fi broblasts was between 20 % and 60 % in all experimental conditions.…”

Section: Chitosan Modified Poly(lactic-co-glycolic) Acid Nanoparticlementioning

confidence: 99%

Chitosan Modified Poly(lactic-Co-Glycolic) Acid Nanoparticles Interaction With Normal, Precancerous Keratinocytes and Dental Pulp Cells

Vîrlan¹,

Calenic²,

Cimpan³

et al. 2017

EDUJ

0

View full text Add to dashboard Cite

Introduction: Nanoparticles (NPs) can carry molecules to different body tissues. Due to their controlled delivery properties, chitosan covered poly-lacto-co-glycolic NPs (PLGAChi NPs) could be used to deliver drugs to oral tissues for the treatment of dental diseases or in anticancer therapy. The aim of this study was to determine the uptake and cytotoxicity of PLGAChi NPs on different types of cells found in the oral cavity. Methodology: Normal oral keratinocytes (NOKs), precancerous keratinocytes (POE9i) and dental pulp cells (DPCs) were exposed for 12h and 24h to 20 g/mL and 200 g/mL PLGAChi NPs covalently tagged with fl uorescein. 3D organotypic tissues of oral mucosa were grown in vitro and exposed to 200g/mL PLGAChi NPs for 24h. Results: Both normal and premalignant oral mucosa cells (NOKs and POE9i) displayed uptake of PLGAChiNPs in a time and concentration-dependent manner, both in 2D and 3D models. A higher and more rapid uptake of PLGAChi NPs by precancerous cell line POE9i was observed when compared to NOKs. Interestingly, DPCs did not display internalized PLGAChi NPs, even at the highest concentration of 200 g/mL. Conclusion: Chitosan-coated PLGAChi NPs proved to be able to cross the cellular membrane of oral keratinocytes, in 2D as well as in 3D cultures. The polymeric NPs used in the present study seem not to be suitable for applications that require NPs uptake by DPCs, as no evidence of uptake in these cells was found in this study. The fi nding that PLGAChi NPs showed signifi cant internalization by human keratinocytes indicate that they could be used for drug delivery purposes to oral mucosa. Keywords: chitosan, PLGAChi, nanoparticles, oral keratinocytes, dental pulp. ABSTRACT

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Nanoparticle-mediated interplay of chitosan and melatonin for improved wound epithelialisation

Cited by 59 publications

References 40 publications

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

New Nanotechnologies for the Treatment and Repair of Skin Burns Infections

Chitosan Modified Poly(lactic-Co-Glycolic) Acid Nanoparticles Interaction With Normal, Precancerous Keratinocytes and Dental Pulp Cells

Chitosan Modified Poly(lactic-Co-Glycolic) Acid Nanoparticles Interaction With Normal, Precancerous Keratinocytes and Dental Pulp Cells

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