Nanoparticle-Mediated Genetic Engineering of Plants

Wang, Jeffrey W.; González-Grandío, Eduardo; Newkirk, Gregory M.; Demirer, Gözde S.; Butrus, Salwan; Giraldo, Juan Pablo; Landry, Markita P.

doi:10.1016/j.molp.2019.06.010

Cited by 70 publications

(53 citation statements)

References 15 publications

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“…Therefore, improving the existing delivery systems and developing new systems will be key to reducing barriers to the inexpensive application of genetic engineering in plants, especially genome editing. Nanomaterials have unique and tunable physical and chemical properties, which can interact with biological matter with exquisite control and precision [168]. Nanoparticles can be used as a carrier system to deliver the genetic materials, such as plasmid DNA, RNA, and oligonucleotides into cells efficiently and rapidly, which reduce the drawbacks and limitations associated with current Agrobacterium -mediated transgene delivery systems [167,168,169].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

“…Nanomaterials have unique and tunable physical and chemical properties, which can interact with biological matter with exquisite control and precision [168]. Nanoparticles can be used as a carrier system to deliver the genetic materials, such as plasmid DNA, RNA, and oligonucleotides into cells efficiently and rapidly, which reduce the drawbacks and limitations associated with current Agrobacterium -mediated transgene delivery systems [167,168,169]. A few successful examples show promise for nanoparticle-mediated passive delivery to plants in vitro [170,171,172] and in vivo [173,174], indicating the potential for passive nanoparticle-mediated delivery with a high efficiency and low toxicity.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

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Citrus Genetic Engineering for Disease Resistance: Past, Present and Future

Sun

Nasrullah

et al. 2019

IJMS

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Worldwide, citrus is one of the most important fruit crops and is grown in more than 130 countries, predominantly in tropical and subtropical areas. The healthy progress of the citrus industry has been seriously affected by biotic and abiotic stresses. Several diseases, such as canker and huanglongbing, etc., rigorously affect citrus plant growth, fruit quality, and yield. Genetic engineering technologies, such as genetic transformation and genome editing, represent successful and attractive approaches for developing disease-resistant crops. These genetic engineering technologies have been widely used to develop citrus disease-resistant varieties against canker, huanglongbing, and many other fungal and viral diseases. Recently, clustered regularly interspaced short palindromic repeats (CRISPR)-based systems have made genome editing an indispensable genetic manipulation tool that has been applied to many crops, including citrus. The improved CRISPR systems, such as CRISPR/CRISPR-associated protein (Cas)9 and CRISPR/Cpf1 systems, can provide a promising new corridor for generating citrus varieties that are resistant to different pathogens. The advances in biotechnological tools and the complete genome sequence of several citrus species will undoubtedly improve the breeding for citrus disease resistance with a much greater degree of precision. Here, we attempt to summarize the recent successful progress that has been achieved in the effective application of genetic engineering and genome editing technologies to obtain citrus disease-resistant (bacterial, fungal, and virus) crops. Furthermore, we also discuss the opportunities and challenges of genetic engineering and genome editing technologies for citrus disease resistance.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Citrus Genetic Engineering for Disease Resistance: Past, Present and Future

Sun

Nasrullah

et al. 2019

IJMS

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“…[ 29 ] For the latest development of NP‐based gene delivery to plants, readers may refer to a recent review [ 30 ] or commentary. [ 31 ]…”

Section: Introductionmentioning

confidence: 99%

Enabling Transgenic Plant Cell–Derived Biomedicines with Nanotechnology

Chiu

Choi

2020

Advanced NanoBiomed Research

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Transgenic plants are promising factories for manufacturing pharmaceutical small molecules or proteins safe for human consumption. Eukaryotic plant cells can synthesize proteins with precise posttranslational modifications, but microbes cannot. Conventional transformation methods (e.g., electroporation, Agrobacterium‐mediated transformation, and biolistic particle delivery) often suffer from inefficient transformation, damage to plant tissues and cells, or applicability to a narrow range of plant species. Notably, the cell wall poses a physical barrier that obstructs effective delivery of nucleic acids to plant cells. Nanoparticles (NPs) are emerging carriers of nucleic acids to plants because they are sufficiently small to diffuse through the cell wall, enter plant cells without the aid of external forces, and inflict limited damage to the plant cells in a broad variety of plants. Herein, three areas of the phytonanotechnology field that merit comprehensive investigations are outlined, namely, the design considerations of NPs for gene delivery to plant cells, homing of NPs to specific organelles (e.g., nucleus and chloroplast), and transformation of plant suspension cells. This perspective concludes with recent insights into scale‐up production of therapeutics by using bioreactors. NPs are poised to catalyze the transformation of plant cells for producing therapeutics in bioreactors at reduced costs, high purity, and improved scale.

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“…Furthermore, the number of plants amenable for organelle genetic engineering is limited 8 . Nanomaterials are emerging as delivery vehicles for biomolecules in plants [9][10][11][12][13] that can be tuned to control their translocation and distribution to plant cells and organelles.…”

mentioning

confidence: 99%

“…Plant nanobiotechnology is a burgeoning field, which aims to develop and apply engineered nanomaterials (ENMs) for engineering and studying plant function 2,10,[14][15][16] . Interfacing ENMs with plants is leading to significant advances towards addressing crucial challenges in plant genetic element delivery 11 , biochemical sensing [17][18][19] , and nutrient and pesticide delivery 20,21 .…”

mentioning

confidence: 99%

Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition motif

et al. 2020

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Current approaches for nanomaterial delivery in plants are unable to target specific subcellular compartments with high precision, limiting our ability to engineer plant function. We demonstrate a nanoscale platform that targets and delivers nanomaterials with biochemicals to plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif. Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles. QD functionalization with β-cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts. Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone. Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.

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Nanoparticle-Mediated Genetic Engineering of Plants

Cited by 70 publications

References 15 publications

Citrus Genetic Engineering for Disease Resistance: Past, Present and Future

Citrus Genetic Engineering for Disease Resistance: Past, Present and Future

Enabling Transgenic Plant Cell–Derived Biomedicines with Nanotechnology

Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition motif

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