Nanoparticle‐mediated double‐stranded RNA delivery system: A promising approach for sustainable pest management

Yan, Shuo; Ren, Binyuan; Shen, Jie

doi:10.1111/1744-7917.12822

Cited by 144 publications

(145 citation statements)

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“…However, there remains the possibility to improve efficacy and speed via co-formulants (e.g. nanoparticles) that enhance efficiency of both dsRNA uptake and subsequent RNAi (Christiaens et al 2020;Yan et al 2020). The benefits of this technology lie in the associated biosafety aspects, due to its mode of action.…”

Section: Future Steps Towards Applicationmentioning

confidence: 99%

Targeting a coatomer protein complex-I gene via RNA interference results in effective lethality in the pollen beetle Brassicogethes aeneus

et al. 2020

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The pollen beetle Brassicogethes aeneus is a serious pest of oilseed rape (Brassica napus) in Europe. Management of this pest has grown difficult due to B. aeneus’s development of resistance to pyrethroid insecticides, as well as the pressure to establish control strategies that minimise the impact on nontarget organisms. RNA interference represents a nucleotide sequence-based, and thus potentially species-specific, approach to agricultural pest control. The present study examined the efficacy of targeting the coatomer gene coatomer subunit alpha (αCOP), via both microinjection and dietary exposure to exogenous complementary dsRNA, on αCOP-silencing and subsequent mortality in B. aeneus. Beetles injected with dsRNA targeting αCOP (at 0.14 µg/mg) showed 88% and 100% mortality at 6 and 10 days post-injection, respectively; where by the same time after dietary exposure, 43%–89% mortality was observed in the 3 µg dsRNA/µL treatment, though the effect was concentration-dependent. Thus, the effect was significant for both delivery routes. In working towards RNA-based management of B. aeneus, future studies should include αCOP as a target of interest.

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Section: Future Steps Towards Applicationmentioning

confidence: 99%

Targeting a coatomer protein complex-I gene via RNA interference results in effective lethality in the pollen beetle Brassicogethes aeneus

et al. 2020

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“…The term ‘nanoparticle’ commonly refers to materials with dimensions between 1 and 100 nm 10–13 . Organic and inorganic nanoparticles that interface with biological systems have been widely applied as drug/gene carriers in biology and in medicine for cancer therapy 14,15 .…”

Section: Introductionmentioning

confidence: 99%

Nanometerization of thiamethoxam by a cationic star polymer nanocarrier efficiently enhances the contact and plant‐uptake dependent stomach toxicity against green peach aphids

Yan

Cheng

Han

et al. 2021

Pest Management Science

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BACKGROUND The utilization efficiency of conventional insecticides is comparatively low in agricultural production, which leads to their excessive application and environmental pollution. Insecticide nanometerization by polymers and polymeric materials has advantages, particularly increased utilization efficiency and reduced insecticide application. RESULTS To increase the utilization efficiency of insecticides, a star polycation (SPc) was selected as a drug carrier that could be complexed with thiamethoxam through electrostatic interaction. Formation of the complex decreased the particle size of thiamethoxam from 575.77 to 116.16 nm in aqueous solution. Plant uptake of SPc‐delivered thiamethoxam was increased 1.69–1.84 times compared with thiamethoxam alone. Nano‐sized thiamethoxam/SPc complexes showed enhanced contact and stomach toxicity against green peach aphids. CONCLUSION SPc is a promising insecticide adjuvant for insecticide nanometerization, and is beneficial in improving insecticidal activity and decreasing the application amounts and application rates of conventional insecticides. © 2020 Society of Chemical Industry

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“…Implementation of crop protection strategies applying RNAi technology are under development (Poelchau et al, 2016;Vogel et al, 2019). Potential applications of environmental or dietary RNAi for pest control include applications of dsRNA in foliar sprays (San Miguel and Scott, 2016); systemic uptake through plant material (Hunter et al, 2012;Dalakouras et al, 2018;Pampolini et al, 2020), oral administration of dsRNA in baits (Zhou et al, 2008), embedded in nanoparticles (Zhang et al, 2010;Yan et al, 2020), expressed in genetically engineered microorganisms (Zhu et al, 2011;Joga et al, 2016), or expressed in transgenic plants (Baum et al, 2007;Mao et al, 2007;Ghag, 2017;Zhang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Emerald Ash Borer Specific Gene Silencing Has No Effect on Non-target Organisms

Pampolini

Rieske

2020

Front. Agron.

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The sequence complementarity of the RNA interference (RNAi) pathway allows for targeted suppression of genes essential for insect survival, and enables development of pest management strategies specific to a given species while reducing the likelihood of adversely impacting non-target organisms (NTOs). The feasibility of manipulating the RNAi pathway to cause mortality in the highly invasive emerald ash borer (EAB) has been demonstrated. Here the spectrum of activity of three double stranded RNAs (dsRNAs) targeting the genes hsp, shi, and sn-rnp in EAB was evaluated in model insects representing five functional guilds including herbivore, predator, detritivore, pollinator, parasitoid; the last represented by the classical biological control agents currently deployed for EAB management in North America. All NTOs were exposed to EAB-specific dsRNAs in diet bioassays that measured potential lethal effects. Gene expression and in silico analysis were also assessed on NTOs for which gene sequences were publicly available. Bioassays demonstrated no lethal effects on our model insects, suggesting a narrow spectrum of activity for the three EAB-specific dsRNAs evaluated. The gene expression and in silico analyses suggest potential sublethal effects on our model pollinator; however we found no effects on insect survival. Overall, our results suggest no adverse effects of the RNAi strategy targeting EAB genes on the survival of the selected non-target organisms we evaluated. The results from this study provide guidance for future RNAi risk analyses that will allow this technology to move forward to a deployment stage.

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Nanoparticle‐mediated double‐stranded RNA delivery system: A promising approach for sustainable pest management

Cited by 144 publications

References 128 publications

Targeting a coatomer protein complex-I gene via RNA interference results in effective lethality in the pollen beetle Brassicogethes aeneus

Targeting a coatomer protein complex-I gene via RNA interference results in effective lethality in the pollen beetle Brassicogethes aeneus

Nanometerization of thiamethoxam by a cationic star polymer nanocarrier efficiently enhances the contact and plant‐uptake dependent stomach toxicity against green peach aphids

Emerald Ash Borer Specific Gene Silencing Has No Effect on Non-target Organisms

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