Nanoparticle-mediated decrease of lamin B1 pools promotes a TRF protein-based adaptive response in cultured cells

Cytotoxic and cytostatic side effects of chitosan nanoparticles as a non-viral gene carrier

“…An up regulation was observed and the most pronounced effect was noted for MDA-MB-231 cell line. It was already shown that NPs can induce both, p21-dependent and p21-independent, premature senescence (Mytych et al, , 2015b. Interestingly, we here report similar observation in the context of CNs-pDNA for the first time.…”

“…On the other side, it is known that many biological effects caused by nanoparticles depend on their size, shape, used dose, behavior of nanoparticles in enviroment (i.e., whether or not their aggregation occurs) and cell type (i.e., suspended versus adherent cell types or healthy versus cancer cell types) (Fu et al, 2014;Mytych et al, 2015aMytych et al, , 2015b. It has been shown that plasmid DNA loaded chitosan nanoparticles (CNs-pDNA) did not cause any cytotoxicity to macrophages (which differentiate from monocytes) (Chellat et al, 2005).…”

“…Since oxidative stress was linked with NPs-mediated toxicity (Manke et al, 2013;Mytych et al, 2015b) and affected cell cycle progression (Boonstra and Post, 2004), we decided to check whether that process also takes place in the applied conditions. Changes in generation of total ROS were monitored for evaluation of CNs-mediated oxidative stress induction in the tested cells and their reduction was observed (Fig.…”

“…Then, cells were washed and suspended in PBS containing 0.1% glucose, 0.5 mM EDTA and 5 mM 2 0 ,7 0 -dichlorodihydrofluorescein diacetate (H 2 DCF-DA) (Sudheesh Kumar et al, 2013). Fluorescence light was monitored in a Tecan Infinite 1 M200 absorbance mode microplate reader at 495 nm (measurement wavelength) and at 525 nm (reference wavelength) (Mytych et al, , 2015b.…”

A novel label-free and reusable electrochemical cytosensor for highly sensitive detection and specific collection of CTCs

“…According to previously reported methods with a little modification Mytych et al, 2015), the procedures can be generally summarized as follows: First of all, removed all medium and washed cells with 0.01 M PBS. Followed, the cells were digested with 0.25% trypsin/EDTA solution for 2-3 min.…”