Nanoparticle Interaction with Biological Membranes: Does Nanotechnology Present a Janus Face?

Leroueil, Pascale R.; Hong, Seungpyo; Mecke, Almut; Baker, James R.; Orr, Bradford G.; Holl, Mark M. Banaszak

doi:10.1021/ar600012y

Cited by 491 publications

(523 citation statements)

References 45 publications

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“…functional nanoscale domains usually rich in cholesterol) is necessary for raft-mediated endocytosis; Rotello et al showed that cholesterol-depleting agents reduce NP uptake independently of NP size and surface charge [23]. Considering experiments on non-biological membranes, cationic dendrimers were shown to induce holes only in fluid phase membranes, not membranes in the gel phase [41]. On the other hand, Granick et al have shown that charged NPs can affect the phase state of lipid membranes, inducing the formation of gel phases in fluid membranes or fluidizing previously gelled membranes [42].…”

Section: Role Of Rafts and Lipid Phasementioning

confidence: 99%

Simulating the interaction of lipid membranes with polymer and ligand-coated nanoparticles

Rossi

Monticelli

2016

Advances in Physics: X

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Nanoscience and nanotechnology are undergoing rapid expansion owing to the promise of breakthroughs in key sectors, such as energy and medicine. As for medicine, interaction of nanomaterials with biological membranes is a key issue, both for the development of drug and gene delivery vectors and for understanding the molecular basis of nanoparticle (NP) biological activity. NP-membrane interactions are often studied with the aid of molecular simulations of model membranes, allowing to overcome the limitations in temporal and spatial resolution generally encountered by experimental techniques applied to fluid membranes. In the present review we summarize the current literature on simulations of NP-membrane interactions, focusing on small polymeric and ligand-coated NPs. Open questions emerging from experiments concern the effect of NP size, surface charge, and ligand arrangement on NP partitioning into and permeation across membranes. While simulations are contributing to significant progress in this area, some challenges remain, namely regarding the representation of the complexity of biological environments and the cooperative behavior of NPs (e.g. aggregation), as well as methodological challenges to tackle the intrinsically multi-scale nature of nano-bio interactions.

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Section: Role Of Rafts and Lipid Phasementioning

confidence: 99%

Simulating the interaction of lipid membranes with polymer and ligand-coated nanoparticles

Rossi

Monticelli

2016

Advances in Physics: X

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“…The G5 dendrimer is widely used in the drug delivery eld, particularly for its nanoscale size that is similar to the biological components of transthyretin (a protein that transports thyroxine and retinol). 32 To investigate the response of an asymmetric membrane to the G5 dendrimer, we simulated the interaction of the G5 dendrimer with asymmetric membranes from both sides of the membrane. During the outer interaction, the Z-distance in the equilibrated state in the membrane became lower with increasing membrane asymmetry levels.…”

Section: Interactions Between the G5 Dendrimer And Asymmetric Membranesmentioning

confidence: 99%

Molecular analysis of interactions between dendrimers and asymmetric membranes at different transport stages

et al. 2014

Soft Matter

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Studying dendrimer-biomembrane interactions is important for understanding drug and gene delivery. In this study, coarse-grained molecular dynamics simulations were performed to investigate the behaviors of polyamidoamine (PAMAM) dendrimers (G4 and G5) as they interacted with asymmetric membranes from different sides of the bilayer, thus mimicking different dendrimer transport stages. The G4 dendrimer could insert into the membrane during an equilibrated state, and the G5 dendrimer could induce pore formation in the membrane when the dendrimers interacted with the outer side (outer interactions) of an asymmetric membrane [with 10% dipalmitoyl phosphatidylserine (DPPS) in the inner leaflet of the membrane]. During the interaction with the inner side of the asymmetric membrane (inner interactions), the G4 and G5 dendrimers only adsorbed onto the membrane. As the membrane asymmetry increased (e.g., increased DPPS percentage in the inner leaflet of the membrane), the G4 and G5 dendrimers penetrated deeper into the membrane during the outer interactions and the G4 and G5 dendrimers were adsorbed more tightly onto the membrane for the inner interactions. When the DPPS content reached 50%, the G4 dendrimer could completely penetrate through the membrane from the outer side to the inner side. Our study provides molecular understanding and reference information about different dendrimer transport stages during drug and gene delivery.

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“…The nonspecific adsorption of charged nanoparticles onto the single-component phospholipid bilayer and the disruption of lipid bilayers have been observed previously. [32][33][34][35][36] The AuNPs used in this work were prepared by citrate reduction of chloroauric acid (HAuCl 4 ) and stabilized in 0.1 mM PBS. 37 The citrate capped AuNPs carry negative surface charges.…”

mentioning

confidence: 99%

Effects of gold nanoparticles on lipid packing and membrane pore formation

Bhat

Edwards

et al. 2016

Applied Physics Letters

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Gold nanoparticles (AuNPs) have been increasingly integrated in biological systems, making it imperative to understand their interactions with cell membranes, the first barriers to be crossed to enter cells. Herein, liposomes composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) as a model membrane system were treated with citrate stabilized AuNPs from 5 to 30 nm at various concentrations. The fluorescence shifts of Laurdan probes reveal that AuNPs in general made liposomes more fluidic. The increased fluidity is expected to result in an increased surface area, and thus liposome shape changes from circular to less circular, which was further confirmed with fluorescence microscopy. The localized stress in lipids induced by electrostatically adsorbed AuNPs was hypothesized to cause the dominant long-range effect of fluidization of unbound lipid membranes. A secondary effect of the AuNP-induced lateral pressure is the membrane rupture or formation of pores, which was probed by AFM under fluid. We found in this study a nanoparticlemediated approach of modulating the stiffness of lipid membranes: by adsorption of AuNPs, lipids at the binding sites are stiffened whereas lipids afar are fluidized. Understanding the factors that modulate lipid packing is important for the discovery of alternative therapeutic methods for diseases linked to membrane integrity such as high blood pressure and cancer metastasis. The size resemblance of nanoparticles (NPs) to biological macromolecules coupled with the NPs' high surface energy leads to intricate interactions between the two entities. Myriads of study have been conducted to gain insights into the bio-nano interface for biomedical applications.

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Nanoparticle Interaction with Biological Membranes: Does Nanotechnology Present a Janus Face?

Cited by 491 publications

References 45 publications

Simulating the interaction of lipid membranes with polymer and ligand-coated nanoparticles

Simulating the interaction of lipid membranes with polymer and ligand-coated nanoparticles

Molecular analysis of interactions between dendrimers and asymmetric membranes at different transport stages

Effects of gold nanoparticles on lipid packing and membrane pore formation

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