Nanoparticle imaging and diagnostic of Caenorhabditis elegans intracellular pH

Mathew, Neal D.; Mathew, Mark D.; Surawski, Peter P. T.

doi:10.1016/j.ab.2014.01.011

Cited by 11 publications

(9 citation statements)

References 42 publications

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“…To overcome The design of fluorescein based ratiometric sensors was applied to the development of novel imaging tools. Mathew et al described a novel nanoprobe for imaging and monitoring pH variations within Caenorhabditis elegans, a nematode usually used as in vitro model organism [98]. Monitoring intestinal intracellular pH of natural strains of C. elegans was quite challenging, because of the presence of a protecting and surrounding cuticle, and selective intestinal uptake [99].…”

Section: Other Applicationsmentioning

confidence: 99%

Fluorescein Derivatives as Fluorescent Probes for pH Monitoring along Recent Biological Applications

Guern

Mussard

Gaucher

et al. 2020

IJMS

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Potential of hydrogen (pH) is one of the most relevant parameters characterizing aqueous solutions. In biology, pH is intrinsically linked to cellular life since all metabolic pathways are implicated into ionic flows. In that way, determination of local pH offers a unique and major opportunity to increase our understanding of biological systems. Whereas the most common technique to obtain these data in analytical chemistry is to directly measure potential between two electrodes, in biological systems, this information has to be recovered in-situ without any physical interaction. Based on their non-invasive optical properties, fluorescent pH-sensitive probe are pertinent tools to develop. One of the most notorious pH-sensitive probes is fluorescein. In addition to excellent photophysical properties, this fluorophore presents a pH-sensitivity around neutral and physiologic domains. This review intends to shed new light on the recent use of fluorescein as pH-sensitive probes for biological applications, including targeted probes for specific imaging, flexible monitoring of bacterial growth, and biomedical applications.

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Section: Other Applicationsmentioning

confidence: 99%

Fluorescein Derivatives as Fluorescent Probes for pH Monitoring along Recent Biological Applications

Guern

Mussard

Gaucher

et al. 2020

IJMS

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“…It should be noted that our observation also explains the incomplete cancellation of uorescein uorescence in pH-sensitive nanoparticles and the variation in the ratiometric signals among different preparations. 31,33,34 Although a pK a value close to the physiological condition is benecial for its subsequent use as a biosensor, its quality is crucially affected by the speed upon which the indicator reacts to changes in pH. The addition of HCl or DCl immediately shis the equilibrium in C-NP, but protons only slowly reach the pHsensitive moiety of 5a in CS-NP ( Fig.…”

Section: Protonation Dynamicsmentioning

confidence: 99%

“…69 Furthermore, the fairly high intensity change upon pH changes simplies the subsequent analyses and can be, at least partially, traced back to the smaller size as compared to other nanoparticle preparations. 31,[34][35][36]85 In addition, the particles consist of hardly toxic components and are smaller than most well-known nanoprobes. Therefore, our particles are ideally suited to track the intracellular pH in living cells as exemplied in live-cellular microscopy.…”

Section: Ratiometric Readoutmentioning

confidence: 99%

“…[29][30][31][32] Although labeled silica nanoparticles with self-calibrating pH detection exist, preferential ratiometric detection is established by a mixture of two or more different uorophores. 22,23,30,31,[33][34][35][36][37][38] In this work, two-channel readout is achieved by a single probe molecule, which provides two reversibly interconverting detection signals. The use of a single probe molecule offers the advantage in which the pH measurement is independent of parameters such as local and irreproducible probe composition or preferential photobleaching of one dye.…”

Section: Introductionmentioning

confidence: 99%

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Kinetic and spectroscopic responses of pH-sensitive nanoparticles: influence of the silica matrix

et al. 2019

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“…To explore this interaction of intracellular pH and metabolic rate, the fluorescent core-silica nanoparticles developed in chapter 3 were used to quantify SO 2 induced pHi changes [3]. In just 2.5…”

Section: Resultsmentioning

confidence: 99%

Developing high-throughput methods for C. elegans to better understand the newly discovered gasotransmitter sulfur dioxide

Mathew¹

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Sulfur Dioxide (SO 2 ) is the most recently discovered gasotransmitter. As a result there is a significant gap in the literature compared to the other established gasotransmitters which have had several decades of research. Initially, SO 2 was thought to be a toxic air pollutant and byproduct from the metabolism of sulphur containing amino acids. It is thus important to understand its mechanism of action and molecular targets of SO 2 . The pathways for metabolism of sulfur containing gasotransmitters (hydrogen sulfide and sulfur dioxide) in lower organisms was previously unknown. My thesis goal is to gain a better understanding of the function and metabolism of SO 2 . To better elucidate this function I have used the eukaryotic model organisms; Saccharomyces cerevisiae (S. cerevisiae) and Caenorhabditis elegans (C. elegans).Two high-throughput methods were developed as part of this research to improve detail and accuracy of analysis in C. elegans. The first method developed was WormScan, which allowed for automatic whole organism phenotyping. The second method developed was pHi nanoparticles, to enable the characterization of intracellular pH (pHi). These two C. elegans methods were published in PLOS ONE, 2012 and Analytical Biochemistry, 2014. WormScan is based on an affordable consumer grade flatbed scanner that facilitates the quantification of the four main toxicological endpoints of C. elegans and greatly reduces the user bias associated with manual counting. The light intensity produced by the scanner was sufficient to cause negative phototaxis equivalent to a physical stimulus for mortality determination. The affordability and high-throughput nature of WormScan also enable high-throughput whole animal drug screening in C. elegans. The pHi nanoparticles were generated through a modified Stöber synthesis and easily calibrated externally to the C. elegans. The nanoparticles were taken up during feeding, and found to bypass the selective intestinal uptake and translocate to the primary organs and secondary organs of the reproductive tract. This was the first diagnostic use of nanoparticles in C. elegans.It was found that the hypoxia-inducible factor-1 (hif-1) transcription factor was required in order for C. elegans to survive exposure to SO 2 . HIF-1 is a regulator of both pHi homeostasis and metabolic rate. When C. elegans are exposed to a non-lethal concentration of SO 2 , it induced a reversible state of suspended animation, a condition that is characterized by metabolic suppression. A physiologically relevant drop of 0.15 pH units was observed in response to SO 2 exposure. Where such a decrease in pHi is a characteristic of reduced metabolism.II A forward genetic screen was carried out in C. elegans to identify mutations that conferred resistance to SO 2 . A resistance mutation was mapped to a region of Chromosome III using an as yet unpublished high-throughput technique (Mip-Map). Together with whole genome sequencing the gene F08F8.9 causing resistance was identified. The F08F8.9 gene is hom...

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Nanoparticle imaging and diagnostic of Caenorhabditis elegans intracellular pH

Cited by 11 publications

References 42 publications

Fluorescein Derivatives as Fluorescent Probes for pH Monitoring along Recent Biological Applications

Fluorescein Derivatives as Fluorescent Probes for pH Monitoring along Recent Biological Applications

Kinetic and spectroscopic responses of pH-sensitive nanoparticles: influence of the silica matrix

Developing high-throughput methods for C. elegans to better understand the newly discovered gasotransmitter sulfur dioxide

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