Nanoparticle embedded enzymes for improved lateral flow sensors

Abstract

In this study, combining the nanoparticle embedded sensors with lateral flow assays, a novel strategy for ensuring the quality of signalling in lateral flow assays (LFAs) was developed. A LFA for reactive oxygen species (ROS) is reported that is based on horse radish peroxidase (HRP) which is co-entrapped with Texas Red dextran inside porous polyacrylamide nanoparticles. In this system, enzymes are protected in the porous matrix of polyacrylamide which freely allows the diffusion of the analyte. The sensor is rapid and sensitive for quantification of hydrogen peroxide concentrations. A test solution of hydrogen peroxides was quantified with this novel LFA-ROS sensor to obtain a linear range between 1 and 25 μM. Nanoparticle embedding of enzymes is proposed here as a general strategy for developing enzyme-based lateral flow assays, eliminating adverse effects associated with biological samples.

Optical biosensor for catechol determination based on laccase‐immobilized anionic polyamide 6 microparticles

“…This strategy has been frequently used for biosensors construction based on polyacrylamide. [27,28] Similarly, the doping of inorganic and hybrid organic-inorganic sol-gel polymers with the bioactive molecule has been widely used. [29,30] Finally, physical adsorption is a simple, low cost, and fast immobilization method that attaches the enzyme to a polymer support via noncovalent interactions, for example, electrostatic, H-bonding, or Van der Waals forces.…”

Optimizing Colorimetric Assay Based on V2O5 Nanozymes for Sensitive Detection of H2O2 and Glucose

“…Until now, several techniques have been developed for a reliable and sensitive determination of H 2 O 2 , such as chemiluminescence [ 23 ], fluorometry [ 24 ], liquid chromatography [ 25 ] and electrochemistry [ 26 ]. Among these methods, sensitive spectrometric methods for the H 2 O 2 determination based on the horseradish peroxidase (HRP)-catalyzed enzymatic reactions have attracted substantial attention owing to the rapid response, and low fabrication cost characteristics [ 27 , 28 ]. Although HRP has high specificity and sensitivity, the application of the free natural enzyme is limited by its poor stability, and high cost [ 29 ].…”

A new point-of-care test for the diagnosis of infectious diseases based on multiplex lateral flow immunoassays

“…8 For example, the lateral flow immunoassay (LFIA) platform is known as an ideal diagnostic test with features including less time consumption, easy operation, durable stability, low cost for POC tests in limited-resource settings and emergency departments. 9 Also, LFIA tests are able to show the existence of pathogens in various biological samples (e.g. whole blood, plasma, serum, saliva, etc.)…”