Nanoparticle coronas take shape

Monopoli, Marco P.; Bombelli, Francesca Baldelli; Dawson, Kenneth A.

doi:10.1038/nnano.2010.267

Cited by 181 publications

(67 citation statements)

References 10 publications

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“…It is now well accepted that nanoparticles acquire a protein corona after contact with biological media [45–47]. This influences their dispersability in biological media, as we have shown for this particular kind of silver nanoparticles, and also their cytotoxicity [48].…”

Section: Reviewmentioning

confidence: 67%

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

Ahlberg¹,

Antonopulos²,

Diendorf³

et al. 2014

Beilstein J. Nanotechnol.

122

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SummaryPVP-capped silver nanoparticles with a diameter of the metallic core of 70 nm, a hydrodynamic diameter of 120 nm and a zeta potential of −20 mV were prepared and investigated with regard to their biological activity. This review summarizes the physicochemical properties (dissolution, protein adsorption, dispersability) of these nanoparticles and the cellular consequences of the exposure of a broad range of biological test systems to this defined type of silver nanoparticles. Silver nanoparticles dissolve in water in the presence of oxygen. In addition, in biological media (i.e., in the presence of proteins) the surface of silver nanoparticles is rapidly coated by a protein corona that influences their physicochemical and biological properties including cellular uptake. Silver nanoparticles are taken up by cell-type specific endocytosis pathways as demonstrated for hMSC, primary T-cells, primary monocytes, and astrocytes. A visualization of particles inside cells is possible by X-ray microscopy, fluorescence microscopy, and combined FIB/SEM analysis. By staining organelles, their localization inside the cell can be additionally determined. While primary brain astrocytes are shown to be fairly tolerant toward silver nanoparticles, silver nanoparticles induce the formation of DNA double-strand-breaks (DSB) and lead to chromosomal aberrations and sister-chromatid exchanges in Chinese hamster fibroblast cell lines (CHO9, K1, V79B). An exposure of rats to silver nanoparticles in vivo induced a moderate pulmonary toxicity, however, only at rather high concentrations. The same was found in precision-cut lung slices of rats in which silver nanoparticles remained mainly at the tissue surface. In a human 3D triple-cell culture model consisting of three cell types (alveolar epithelial cells, macrophages, and dendritic cells), adverse effects were also only found at high silver concentrations. The silver ions that are released from silver nanoparticles may be harmful to skin with disrupted barrier (e.g., wounds) and induce oxidative stress in skin cells (HaCaT). In conclusion, the data obtained on the effects of this well-defined type of silver nanoparticles on various biological systems clearly demonstrate that cell-type specific properties as well as experimental conditions determine the biocompatibility of and the cellular responses to an exposure with silver nanoparticles.

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Section: Reviewmentioning

confidence: 67%

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

Ahlberg¹,

Antonopulos²,

Diendorf³

et al. 2014

Beilstein J. Nanotechnol.

122

View full text Add to dashboard Cite

show abstract

“…When nanoparticles are introduced into biological systems, proteins would interact with nanoparticles and lead to the formation of so called "protein corona" on the surface of the nanoparticles. As reported previously, the protein corona on nanoparticles would critically impact the biological identities of nanoparticles [6][7][8][9] and/or result in the physiological and pathological consequences of the macrophage uptake, blood coagulation, complement activation and cellular toxicity, etc. [10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 84%

The on-bead digestion of protein corona on nanoparticles by trypsin immobilized on the magnetic nanoparticle

Hu¹,

Zhao²,

Zhang³

et al. 2014

Journal of Chromatography A

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“…The composition, concentration, surface charge, and size of the nanomaterials significantly influence the way of their interaction with the biomolecules, which may occasionally be irreproducible, leading to different responses from the similar treatments [1]. This complication originates from the fact that every biological environment has a unique character that should be adequately exploited for each nanomaterial to be exposed, which could facilitate the development of novel nanomaterials with unique properties structured for the application of interest.…”

Section: Introductionmentioning

confidence: 99%

Amplification yield enhancement of short DNA templates using bulk and surface-attached amine-functionalized single-wall carbon nanotubes

Yüce

Uysal

Budak

2015

Applied Surface Science

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Nanoparticle coronas take shape

Cited by 181 publications

References 10 publications

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

The on-bead digestion of protein corona on nanoparticles by trypsin immobilized on the magnetic nanoparticle

Amplification yield enhancement of short DNA templates using bulk and surface-attached amine-functionalized single-wall carbon nanotubes

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