Nanoparticle-based electrochemiluminescence immunosensor with enhanced sensitivity for cardiac troponin I using N-(aminobutyl)-N-(ethylisoluminol)-functionalized gold nanoparticles as labels

Shen, Wen; Tian, Dayong; Cui, Hua; Yang, Di; Bian, Zhiping

doi:10.1016/j.bios.2011.05.022

Cited by 121 publications

(47 citation statements)

References 38 publications

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“…60,61 A novel nanoparticle based on an ECL immunosensor has been employed for sensitive detection of human cTnI. 22,62 The production of luminescence during electrochemical reaction is considered electrochemiluminescence. A high-sensitivity, wide-linear, low-background, and simple instrument that can be used for detecting the acute myocardial infarction biomarkers are taken into account as the positive characteristics of ECL.…”

Section: Luminescence and Colorimetric Methods For Cardiac Troponin Dmentioning

confidence: 99%

“…Gold nanoparticles were functionalized by using N-(aminobutyl)-N-(ethylisoluminol) (ABEI)-AuNPs and are used as labels via the simple seed growth method for ultrasensitive detection of cTnI in clinical diagnosis. 62 Two kinds of nanoparticles, SA-AuNPs (streptavidin-coated gold nanoparticles) and ABEI-AuNPs-Ab2 nanoprobes, were used for the ECL immunosensor. SA-AuNPs enhance the conductivity or electron transfer between biomolecules and electrode and also improve immobilized capacity.…”

Section: Luminescence and Colorimetric Methods For Cardiac Troponin Dmentioning

confidence: 99%

“…85 This technique is of high interest due to its high sensitivity and potential for multiplexing. 62,86,87 Because Raman peaks are much narrower than fluorescence bands, SERS has potential for multiplex detection. 88 SERS assay was used recently via SERS nano-tags and magnetic beads for a sandwich immunoassay.…”

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confidence: 99%

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Development of optical biosensor technologies for cardiac troponin recognition

Abdolrahim

Rabiee

Alhosseini

et al. 2015

Analytical Biochemistry

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Section: Luminescence and Colorimetric Methods For Cardiac Troponin Dmentioning

confidence: 99%

Section: Luminescence and Colorimetric Methods For Cardiac Troponin Dmentioning

confidence: 99%

See 1 more Smart Citation

Development of optical biosensor technologies for cardiac troponin recognition

Abdolrahim

Rabiee

Alhosseini

et al. 2015

Analytical Biochemistry

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“…Moreover, Abad et al have demonstrated that cTnT can be determined in the range of 0.05-1.0 ng/mL using a cyclo-olefin polymer based microfluidic device for capturing magnetic beads and performing electrochemical detection (63). A detection limit of 2 pg/mL for cTnI was reached by using nanoparticle-based electrochemiluminescence immunosensor labels in a high sensitive sandwich-type immunoassay (64).…”

Section: Sst2 Gdf-15 Hstn Imentioning

confidence: 99%

Advances in the detection and quantification of candidate and established biomarkers in heart failure

Pop¹,

Crișan²,

Loghin³

et al. 2013

Romanian Review of Laboratory Medicine

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“…Several ECL methods have been developed for the determination of TnI [11][12][13][14][15]. Cui designed ECL immunosensor for the detection of human cardiac troponin I by using luminol [11] and N-(aminobutyl)-N-(ethylisoluminol) [12] -functionalized gold nanoparticles as labels. Smith developed an ECL immunoassay for detection of rat TnI in serum [13].…”

Section: Introductionmentioning

confidence: 99%

Sensitive electrogenerated chemiluminescence peptide-based biosensor for the determination of troponin I with gold nanoparticles amplification

Meng

Qiu

et al. 2013

Gold Bull

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A sensitive electrogenerated chemiluminescence (ECL) peptide-based biosensor was fabricated for the determination of troponin I (TnI) by employing gold nanoparticles as amplification platform. Two specific peptides including peptide1 with a sequence of CFYSHSFHENWPS and peptide2 with a sequence of FYSHSFHENWPSK were employed as capture peptide and report peptide, respectively. The peptide2 was labeled with ruthenium bis(2,2′-bipyridine) (2,2′-bipyridine-4,4′-dicarboxylic acid)-N-hydroxysuccinimide ester (Ru(bpy) 2 (dcbpy)NHS) at NH 2 -containing lysine via acylation reaction and utilized as the ECL probe. Gold nanoparticles were electrodeposited onto gold electrode and used as an amplification platform. The peptide-based biosensor was fabricated by self-assembling peptide1 onto the surface of gold nanoparticles-modified gold electrode through a thiolcontaining cysteine at the end of the peptide1. When the biosensor reacted with target TnI, and then incubated with the ECL probe, a strong ECL response was electrochemically generated. The ECL intensity is directly proportional to the logarithm of the concentration of TnI in the range from 1 to 300 pg/mL. The biosensor employing gold nanoparticles as amplification platform shows high sensitivity for the detection of TnI with a detection limit of 0.4 pg/mL (S/N =3). Moreover, the biosensor is successfully applied to analysis of TnI in human serum sample. This work demonstrates that the combination of a highly binding peptide with nanoparticle amplification is a great promising approach for the design of ECL biosensor.

show abstract

Nanoparticle-based electrochemiluminescence immunosensor with enhanced sensitivity for cardiac troponin I using N-(aminobutyl)-N-(ethylisoluminol)-functionalized gold nanoparticles as labels

Cited by 121 publications

References 38 publications

Development of optical biosensor technologies for cardiac troponin recognition

Development of optical biosensor technologies for cardiac troponin recognition

Advances in the detection and quantification of candidate and established biomarkers in heart failure

Sensitive electrogenerated chemiluminescence peptide-based biosensor for the determination of troponin I with gold nanoparticles amplification

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