Nanoparticle-Based Contrast Agents for<sup>129</sup>Xe HyperCEST NMR and MRI Applications

Jayapaul, Jabadurai; Schröder, Leif

doi:10.1155/2019/9498173

Cited by 14 publications

(9 citation statements)

References 195 publications

(212 reference statements)

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“…Nanoparticles, generally characterized by submicronic (<1 µm) systems of metallic or organic materials, offer broad applications in various fields including tumor detection, imaging, diagnosis and treatment 56 . Several types of nanoparticles, such as carbonaceous, Prussian blue, or paramagnetic nanoparticles are available for in vitro diagnosis based on their peroxidase-like activity or function as enzyme carriers 57 , 58 and in vivo diagnosis as carriers of contrast agents 59 , 60 . In addition to diagnosis, their current applications in cancer treatment have gained widespread interest.…”

Section: Discussionmentioning

confidence: 99%

Ferumoxytol-β-glucan Inhibits Melanoma Growth via Interacting with Dectin-1 to Polarize Macrophages into M1 Phenotype

Liu¹,

Xu²,

Li³

et al. 2021

Int. J. Med. Sci.

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Background: Regulating the polarization of macrophages to antitumor M1 macrophages is a promising strategy for overcoming the immunosuppression of the tumor microenvironment for cancer therapy. Ferumoxytol (FMT) can not only serve as a drug deliver agent but also exerts anti-tumor activity. β-glucan has immuno-modulating properties to prevent tumor growth. Thus, a nanocomposite of FMT surface-coated with β-glucan (FMT-β-glucan) was prepared to explore its effect on tumor suppression. Methods:Male B16F10 melanoma mouse model was established to explore the antitumor effect of FMT-β-glucan. The viability and apoptotic rates of B16F10 cells were detected by cell counting kit-8 and Annexin-V/PI experiments. The levels of M1 markers were quantified by quantitative reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay. Phagocytic activity and intracellular reactive oxygen species (ROS) in macrophages were evaluated by the neutral red uptake assay and flow cytometry, respectively. Small interfering RNA (siRNA) transfection was applied to knock down the Dectin-1 gene in RAW 264.7 cells.Results: FMT-β-glucan suppressed tumor growth to a greater extent and induced higher infiltration of M1 macrophages than the combination of FMT and β-glucan (FMT+β-glucan) in vivo. In vitro, supernatant from FMT-β-glucan-treated RAW 264.7 cells led to lower cell viability and induced more apoptosis of B16F10 cells than that from the FMT+β-glucan group. Moreover, FMT-β-glucan boosted the expression of M1 type markers, and increased phagocytic activity and ROS in RAW 264.7 cells. Further research indicated that FMT-β-glucan treatment promoted the level of Dectin-1 on the surface of RAW 264.7 cells and that knockdown of Dectin-1 abrogated the phosphorylation levels of several components in MAPK and NF-κB signaling. Conclusion:The nanocomposite FMT-β-glucan suppressed melanoma growth by inducing the M1 macrophage-activated tumor microenvironment.

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Section: Discussionmentioning

confidence: 99%

Ferumoxytol-β-glucan Inhibits Melanoma Growth via Interacting with Dectin-1 to Polarize Macrophages into M1 Phenotype

Liu¹,

Xu²,

Li³

et al. 2021

Int. J. Med. Sci.

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“…Chemical exchange saturation transfer (CEST) and HyperCEST imaging are newer MRI modalities using reagents based on xenon in which exogenous nuclei are selectively saturated and enhanced water signals are observed due to saturation transfer to the surrounding bulk water (120,121). Bacteriophage MS2, M13, and fd have been used to develop VNP/VLP formulations to exploit these new techniques.…”

Section: Viruses and Virus-like Particles For Molecular Imagingmentioning

confidence: 99%

Plant Viruses and Bacteriophage-Based Reagents for Diagnosis and Therapy

Shukla

Cai

et al. 2020

Annu. Rev. Virol.

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Viral nanotechnology exploits the prefabricated nanostructures of viruses, which are already abundant in nature. With well-defined molecular architectures, viral nanocarriers offer unprecedented opportunities for precise structural and functional manipulation using genetic engineering and/or bio-orthogonal chemistries. In this manner, they can be loaded with diverse molecular payloads for targeted delivery. Mammalian viruses are already established in the clinic for gene therapy and immunotherapy, and inactivated viruses or virus-like particles have long been used as vaccines. More recently, plant viruses and bacteriophages have been developed as nanocarriers for diagnostic imaging, vaccine and drug delivery, and combined diagnosis/therapy (theranostics). The first wave of these novel virus-based tools has completed clinical development and is poised to make an impact on clinical practice.

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“…This allows unprecedented minimally invasive cell labelling for 129 Xe NMR detection. It combines the advantage of non-ionizing radiation with a unique reporter approach by incorporating emerging aspects of nanotechnology [9] for improved contrast generation.…”

Section: Doi: 101002/adbi201900251mentioning

confidence: 99%

Functionalized Lipopeptide Micelles as Highly Efficient NMR Depolarization Seed Points for Targeted Cell Labelling in Xenon MRI

et al. 2020

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Improving diagnostic imaging and therapy by targeted compound delivery to pathological areas and across biological barriers is of urgent need. A lipopeptide, P‐CrA‐A2, composed of a highly cationic peptide sequence (A2), an N‐terminally attached palmitoyl chain (P) and cryptophane molecule (CrA) for preferred uptake into blood–brain barrier (BBB) capillary endothelial cells, was generated. CrA allows reversible binding of Xe for NMR detection with hyperpolarized nuclei. The lipopeptide forms size‐optimized micelles with a diameter of about 11 nm at low micromolar concentration. Their high local CrA payload has a strong and switchable impact on the bulk magnetization through Hyper‐CEST detection. Covalent fixation of CrA does not impede micelle formation and does not hamper its host functionality but simplifies Xe access to hosts for inducing saturation transfer. Xe Hyper‐CEST magnetic resonance imaging (MRI) allows for distinguishing BBB endothelial cells from control aortic endothelial cells, and the small micelle volume with a sevenfold improved CrA‐loading density compared to liposomal carriers allows preferred cell labelling with a minimally invasive volume (≈16 000‐fold more efficient than 19F cell labelling). Thus, these nanoscopic particles combine selectivity for human brain capillary endothelial cells with great sensitivity of Xe Hyper‐CEST MRI and might be a potential MRI tool in brain diagnostics.

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Nanoparticle-Based Contrast Agents for¹²⁹Xe HyperCEST NMR and MRI Applications

Cited by 14 publications

References 195 publications

Ferumoxytol-β-glucan Inhibits Melanoma Growth via Interacting with Dectin-1 to Polarize Macrophages into M1 Phenotype

Ferumoxytol-β-glucan Inhibits Melanoma Growth via Interacting with Dectin-1 to Polarize Macrophages into M1 Phenotype

Plant Viruses and Bacteriophage-Based Reagents for Diagnosis and Therapy

Functionalized Lipopeptide Micelles as Highly Efficient NMR Depolarization Seed Points for Targeted Cell Labelling in Xenon MRI

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Nanoparticle-Based Contrast Agents for129Xe HyperCEST NMR and MRI Applications

Cited by 14 publications

References 195 publications

Ferumoxytol-β-glucan Inhibits Melanoma Growth via Interacting with Dectin-1 to Polarize Macrophages into M1 Phenotype

Ferumoxytol-β-glucan Inhibits Melanoma Growth via Interacting with Dectin-1 to Polarize Macrophages into M1 Phenotype

Plant Viruses and Bacteriophage-Based Reagents for Diagnosis and Therapy

Functionalized Lipopeptide Micelles as Highly Efficient NMR Depolarization Seed Points for Targeted Cell Labelling in Xenon MRI

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Product

Resources

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Nanoparticle-Based Contrast Agents for¹²⁹Xe HyperCEST NMR and MRI Applications